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[178921_at]

Description ZK856.3 /REP_DB=WormBase Gene ID /WP=CE06660 /TR=Q23637 /GB=CAA94850.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 09      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 64
(-1.3)
A 		70.8
(-0.6)
A 		47.8
(-3)
A 		104.5
(3)
A 		57
B 41.5
(-3)
A 		88.6
(2.8)
A 		90.3
(3)
A 		59.9
(-0.7)
A 		49
C 75.2
(-0.4)
A 		36.5
(-3)
A 		55.3
(-1.7)
A 		124.4
(3)
A 		88
avr 60.2
(-3) 		65.3
(-2.2) 		64.5
(-2.3) 		96.3
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 108
(3)
A 		12.5
(-2.8)
A 		8.5
(-3)
A 		10.7
(-2.9)
A 		97.5
(2.4)
A 		78.1
(1.2)
A 		100
B 13.7
(-3)
A 		54.3
(-0.6)
A 		79.7
(0.8)
A 		27.3
(-2.2)
A 		76.6
(0.7)
A 		116.9
(3)
A 		103
C 26.2
(-1.9)
A 		76.6
(0.8)
A 		75.4
(0.8)
A 		5.9
(-3)
A 		78.1
(0.9)
A 		116.6
(3)
A 		111
avr 49.3
(-0.7) 		47.8
(-0.8) 		54.5
(-0.3) 		14.6
(-3) 		84.1
(1.7) 		103.9
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 23.3
(3)
A 		15.4
(-1.4)
A 		12.5
(-3)
A 		15.3
(-1.4)
A 		11
B 13.2
(-2.7)
A 		21.9
(3)
A 		20.9
(2.3)
A 		12.8
(-3)
A 		9
C 24.9
(3)
A 		10.5
(-3)
A 		19.3
(0.7)
A 		21
(1.4)
A 		14
avr 20.5
(3) 		15.9
(-3) 		17.6
(-0.8) 		16.4
(-2.3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 19.9
(0.9)
A 		4.4
(-2.4)
A 		1.8
(-3)
A 		2.7
(-2.8)
A 		29.4
(3)
A 		19
(0.7)
A 		28
B 4.8
(-3)
A 		18.1
(-0.9)
A 		19.4
(-0.7)
A 		7.2
(-2.6)
A 		31.1
(1.1)
A 		43.6
(3)
A 		39
C 9.7
(-1.7)
A 		23.4
(0.5)
A 		19.2
(-0.1)
A 		1.2
(-3)
A 		25.3
(0.8)
A 		39
(3)
A 		38
avr 11.5
(-1.5) 		15.3
(-0.7) 		13.5
(-1.1) 		3.7
(-3) 		28.6
(1.9) 		33.9
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178921_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11976
Sequence Type Exemplar sequence
Representative Public ID ZK856.3 NCBI
Target Description ZK856.3 /REP_DB=WormBase Gene ID /WP=CE06660 /TR=Q23637 /GB=CAA94850.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073219(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073219 NCBI Caenorhabditis elegans ZK856.5 (ZK856.5) mRNA, complete cds. 11/11 None
SNAP00000036947 ENSEMBL cdna:SNAP chromosome:CEL140:V:10187331:10192698:-1 11/11 None
GENEFINDER00000036970 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10187331:10188569:-1 11/11 None
ZK856.5 ENSEMBL cdna:known chromosome:CEL140:V:10187331:10192698:-1 gene:ZK856.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10187330-10189758(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK856.5
Entrez Gene 191439 Entrez gene
SwissProt Q23640 EMBL-EBI
WormBase CE38478 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.18594.1.S1_AT similar to acid sphingomyelinase-like phosphodiesterase 3B isoform 1 cfa
DROSOPHILA_2:1638906_AT dm
CHICKEN:GGA.6971.1.S1_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.1.S1_S_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGAAFFX.21962.1.S1_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.2.S1_AT Sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.2.A1_AT Sphingomyelin phosphodiesterase, acid-like 3B gga
HG-U133_PLUS_2:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133A:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-FOCUS:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133A_2:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HU35KSUBA:Y08134_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HUGENEFL:Y08134_AT sphingomyelin phosphodiesterase, acid-like 3B hs
U133_X3P:G7656907_3P_AT sphingomyelin phosphodiesterase, acid-like 3B hs
U133_X3P:HS2.123659.2.S1_3P_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133_PLUS_2:1554050_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U95AV2:37779_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U95C:64012_AT sphingomyelin phosphodiesterase, acid-like 3B hs
MG-U74BV2:115354_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOE430A:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOUSE430A_2:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOUSE430_2:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
Protein Similarities
Method ID Description E-Value
blast CAA94853 Hypothetical protein ZK856.5 [Caenorhabditis elegans] ref|NP_505620.3| ZK856.5 [Caenorhabditis elegans] 0.0
blast T28041 hypothetical protein ZK856.3 - Caenorhabditis elegans 0.0
blast CAE64751 Hypothetical protein CBG09540 [Caenorhabditis briggsae] 0.0
blast AAA68416 Hypothetical protein F18C5.7 [Caenorhabditis elegans] 0.0
blast F89402 protein Y94A7B.2 [imported] - Caenorhabditis elegans 0.0
blast AAB66191 Hypothetical protein T07D3.8 [Caenorhabditis elegans] 0.0
blast CAA94853 Hypothetical protein ZK856.5 [Caenorhabditis elegans] ref|NP_505620.3| ZK856.5 [Caenorhabditis elegans] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.1E-11
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 8.0E-12
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 0.0017
Pfam IPR002492 EMBL-EBI Transposase, Tc1/Tc3 4.3E-35
Sequence
Target Sequence 
>C. ELEGANS:178921_AT
aaagatctgcccgacagtctgtatctcggcttttggaactaacttcaggaactatgtttg
tgaatggatatatgtggacaatttgcagaccagtcaaatgcaatcacgtcgcgagaactc
atgaagagagagaagttgaagaaccaggaatcacgacttcacgtggagtaactgttgatg
aacttagtgggatctttgcaagttttattcaacaaaattcttcggtttcccaaacatcaa
ttttctctgattcgactcaatccttctcgtcatattcattgttctctcggtcaagaaccg
ttagctcatcgtcctctcaatacgctgaaagttgcgattcgtctgcaacaatcactccgt
tttcatactatcccgcacaacaatcaaccggttttctcaatcttcctccaccttatcaca
ctcttccggatactgctttcttccgtcgcacgaaaatcatccctcctccgccaatgagac
aagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGATCTGCCCGACAGTCTGTATC 327 123 1097 Antisense
AATGCAATCACGTCGCGAGAACTCA 584 165 1193 Antisense
AGAACCAGGAATCACGACTTCACGT 181 73 1236 Antisense
GGTTTCCCAAACATCAATTTTCTCT 127 437 1320 Antisense
TTCTCTGATTCGACTCAATCCTTCT 135 693 1339 Antisense
CGTCATATTCATTGTTCTCTCGGTC 252 247 1364 Antisense
TCAAGAACCGTTAGCTCATCGTCCT 429 629 1387 Antisense
TCATCGTCCTCTCAATACGCTGAAA 401 619 1402 Antisense
CAATCACTCCGTTTTCATACTATCC 137 181 1445 Antisense
TCTTCCGTCGCACGAAAATCATCCC 107 617 1535 Antisense
CCCTCCTCCGCCAATGAGACAAGAA 139 261 1557 Antisense
Sequence Source Affymetrix Proprietary Database