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[178896_at]

Description C25F9.2 /REP_DB=WormBase Gene ID /WP=CE17467 /TR=O62074 /GB=CAB03918.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 87.3
(0.8)
P 		72.2
(-0.6)
A 		45
(-3)
A 		112.3
(3)
A 		67
B 104
(3)
P 		42.5
(-0.9)
A 		10.5
(-3)
A 		40.3
(-1.1)
A 		94
C 82.6
(2.1)
P 		49.6
(-0.2)
A 		10.8
(-3)
A 		94.5
(3)
P 		84
avr 91.3
(3) 		54.8
(-0.2) 		22.1
(-3) 		82.4
(2.2) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 53.2
(1.5)
A 		21.2
(-2.6)
A 		64.7
(3)
M 		39.2
(-0.3)
A 		18.5
(-3)
A 		21.4
(-2.6)
A 		46
B 47.3
(1.4)
A 		34.6
(-3)
A 		36.1
(-2.5)
A 		45.2
(0.7)
A 		39.3
(-1.4)
A 		51.9
(3)
A 		17
C 22
(-1.1)
A 		66
(3)
M 		44
(0.9)
A 		1.9
(-3)
A 		15.9
(-1.7)
A 		39.5
(0.5)
A 		64
avr 40.8
(1.1) 		40.6
(1.1) 		48.3
(3) 		28.8
(-1.9) 		24.6
(-3) 		37.6
(0.3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 31.8
(3)
P 		15.7
(-1.8)
A 		11.8
(-3)
A 		16.4
(-1.6)
A 		20
B 33.2
(3)
P 		10.5
(-1.4)
A 		2.4
(-3)
A 		8.6
(-1.8)
A 		31
C 27.4
(3)
P 		14.3
(-0.3)
A 		3.8
(-3)
A 		15.9
(0.1)
P 		24
avr 30.8
(3) 		13.5
(-1.2) 		6
(-3) 		13.6
(-1.2) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.8
(0.1)
A 		7.5
(-1.4)
A 		14.1
(3)
M 		9.9
(0.2)
A 		5.6
(-2.7)
A 		5.2
(-3)
A 		9
B 16.4
(1.3)
A 		11.5
(-1.5)
A 		8.8
(-3)
A 		12
(-1.2)
A 		16
(1.1)
A 		19.3
(3)
A 		11
C 8.2
(-0.6)
A 		20.1
(3)
M 		11.2
(0.3)
A 		0.4
(-3)
A 		5.1
(-1.6)
A 		13.2
(0.9)
A 		20
avr 11.5
(1.4) 		13
(3) 		11.4
(1.3) 		7.4
(-3) 		8.9
(-1.4) 		12.6
(2.6) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178896_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14030
Sequence Type Exemplar sequence
Representative Public ID C25F9.2 NCBI
Target Description C25F9.2 /REP_DB=WormBase Gene ID /WP=CE17467 /TR=O62074 /GB=CAB03918.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075379(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075379 NCBI Caenorhabditis elegans C25F9.2 (C25F9.2) mRNA, complete cds. 11/11 None
SNAP00000028318 ENSEMBL cdna:SNAP chromosome:CEL140:V:19424044:19429286:1 11/11 None
GENEFINDER00000028329 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19424044:19429286:1 11/11 None
C25F9.2 ENSEMBL cdna:known chromosome:CEL140:V:19424044:19429286:1 gene:C25F9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19424043-19429286(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25F9.2
Entrez Gene 182904 Entrez gene
SwissProt O62074 EMBL-EBI
WormBase CE17467 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6260 DNA replication inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3887 DNA-directed DNA polymerase activity inferred from electronic annotation QuickGO AmiGO
8408 3'-5' exonuclease activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03918 Hypothetical protein C25F9.2 [Caenorhabditis elegans] ref|NP_507780.1| C25F9.2 [Caenorhabditis elegans] 0.0
blast XP_793143 PREDICTED: similar to predicted CDS, DNA polymerase type B, organellar and viral family member (5T899) [Strongylocentrotus purpuratus] 1.0E-89
blast XP_793143 PREDICTED: similar to predicted CDS, DNA polymerase type B, organellar and viral family member (5T899) [Strongylocentrotus purpuratus] 4.0E-87
blast XP_793143 PREDICTED: similar to predicted CDS, DNA polymerase type B, organellar and viral family member (5T899) [Strongylocentrotus purpuratus] 1.0E-84
blast CAD60788 novel protein similar to DNA polymerases [Danio rerio] ref|NP_001004509.1| hypothetical protein LOC368518 [Danio rerio] 5.0E-82
blast CAB05717 Hypothetical protein E01G4.5 [Caenorhabditis elegans] ref|NP_496835.1| E01G4.5 [Caenorhabditis elegans] 7.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR004868 EMBL-EBI DNA polymerase type B, organellar and viral 5.9E-111
Pfam IPR004868 EMBL-EBI DNA polymerase type B, organellar and viral 4.9E-91
Pfam IPR004868 EMBL-EBI DNA polymerase type B, organellar and viral 1.2E-11
Pfam IPR004868 EMBL-EBI DNA polymerase type B, organellar and viral 1.3E-95
Pfam IPR004868 EMBL-EBI DNA polymerase type B, organellar and viral 1.2E-11
Sequence
Target Sequence 
>C. ELEGANS:178896_AT
agttccattcaccaatcttgctattgcagcgatcaccacatcagcaggtcgtctactgct
tttggaggcaatggagaaagtaggaattgagaaccttatctattgcgatactgactcgct
aatcttcaaacaaatccaagggatggatccactgggagacatgaaaggcacacagctagg
agctctaaccaacgagatcccagaaggaaagaagctagtagaggtagtgtgtatgacacc
aaaggtgtattcactaaagatggaagatgttaatggtgaattggactacacagtaaaggc
gaagggtatgaccttgaactacggaaacgcccaaattattaaccatacaagtatggctga
aagaatgcatgacttcaacacaaagggaacatcagaaccacttcaaggtgaaatgttcac
attcaaacggggagacaatatgctagacgggctctacacatacacaaccaagaaaaatct
gaacccacgtatggataagggtcactacaacacggagggtgtggtcacaccatttgggtg
gcagccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCCATTCACCAATCTTGCTATT 222 65 3834 Antisense
TTGCTATTGCAGCGATCACCACATC 478 701 3851 Antisense
TCACCACATCAGCAGGTCGTCTACT 116 625 3866 Antisense
GGTCGTCTACTGCTTTTGGAGGCAA 499 499 3880 Antisense
GATACTGACTCGCTAATCTTCAAAC 72 423 3940 Antisense
GGCACACAGCTAGGAGCTCTAACCA 420 535 4000 Antisense
AGCTCTAACCAACGAGATCCCAGAA 583 77 4014 Antisense
TGACCTTGAACTACGGAAACGCCCA 493 567 4142 Antisense
TGCTAGACGGGCTCTACACATACAC 95 581 4274 Antisense
TAAGGGTCACTACAACACGGAGGGT 226 635 4329 Antisense
GGTCACACCATTTGGGTGGCAGCCG 432 503 4356 Antisense
Sequence Source Affymetrix Proprietary Database