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[178876_at]

Description F56A8.5 /REP_DB=WormBase Gene ID /WP=CE16125 /TR=O45576 /GB=CAB05745.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 92      6grp_total > 86      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1672
1 2 3 4 range
A 285.8
(-2.1)
P 		125.9
(-3)
P 		164
(-2.8)
P 		1234.6
(3)
P 		1109
B 248.1
(-2.6)
P 		146.8
(-3)
P 		137.2
(-3)
P 		1797.9
(3)
P 		1661
C 298.8
(-2.3)
P 		145
(-3)
P 		198
(-2.8)
P 		1526.2
(3)
P 		1381
avr 277.6
(-2.4) 		139.2
(-3) 		166.4
(-2.9) 		1519.6
(3) 		1380

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 385
1 2 3 4 5 6 range
A 158.9
(-3)
P 		192.9
(-2.1)
P 		398.3
(3)
P 		271.7
(-0.2)
P 		283.8
(0.1)
P 		363.8
(2.1)
P 		239
B 336.7
(-1.3)
P 		294.6
(-2.1)
P 		252.9
(-3)
P 		319.4
(-1.6)
P 		384.7
(-0.3)
P 		544.3
(3)
P 		291
C 231.6
(-2.7)
P 		255.7
(-2.3)
P 		217.9
(-3)
P 		397.6
(0.4)
P 		360.3
(-0.3)
P 		536.8
(3)
P 		319
avr 242.4
(-3) 		247.7
(-2.9) 		289.7
(-1.8) 		329.6
(-0.8) 		342.9
(-0.5) 		481.6
(3) 		239
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 356
1 2 3 4 range
A 104
(0)
P 		27.3
(-3)
P 		42.9
(-2.4)
P 		180.7
(3)
P 		153
B 79.1
(-2.2)
P 		36.3
(-2.9)
P 		31.8
(-3)
P 		383.1
(3)
P 		351
C 98.9
(-1.4)
P 		41.7
(-3)
P 		69
(-2.2)
P 		257.3
(3)
P 		216
avr 94
(-1.5) 		35.1
(-3) 		47.9
(-2.7) 		273.7
(3) 		239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 29.3
(-3)
P 		68.6
(1)
P 		86.7
(2.8)
P 		68.9
(1)
P 		85.6
(2.7)
P 		88.6
(3)
P 		59
B 117.2
(-0.6)
P 		98.1
(-1.4)
P 		61.4
(-3)
P 		84.6
(-2)
P 		156.3
(1)
P 		203
(3)
P 		142
C 85.8
(-1.5)
P 		78
(-1.9)
P 		55.5
(-3)
P 		80
(-1.8)
P 		116.6
(-0)
P 		179.7
(3)
P 		124
avr 77.4
(-2.4) 		81.6
(-2.1) 		67.9
(-3) 		77.8
(-2.3) 		119.5
(0.5) 		157.1
(3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178876_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6796
Sequence Type Exemplar sequence
Representative Public ID F56A8.5 NCBI
Target Description F56A8.5 /REP_DB=WormBase Gene ID /WP=CE16125 /TR=O45576 /GB=CAB05745.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067332(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067332 NCBI Caenorhabditis elegans F56A8.5 (F56A8.5) mRNA, complete cds. 11/11 None
SNAP00000043512 ENSEMBL cdna:SNAP chromosome:CEL140:III:13262835:13266401:1 11/11 None
GENEFINDER00000043518 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13265388:13266401:1 11/11 None
F56A8.5 ENSEMBL cdna:known chromosome:CEL140:III:13265387:13266433:1 gene:F56A8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13265383-13266397(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F56A8.5
Entrez Gene 186356 Entrez gene
SwissProt O45576 EMBL-EBI
WormBase CE16125 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05745 Hypothetical protein F56A8.5 [Caenorhabditis elegans] ref|NP_499733.1| F56A8.5 [Caenorhabditis elegans] 1.0E-178
blast CAB05745 Hypothetical protein F56A8.5 [Caenorhabditis elegans] ref|NP_499733.1| F56A8.5 [Caenorhabditis elegans] 1.0E-164
blast CAB05744 Hypothetical protein F56A8.4 [Caenorhabditis elegans] ref|NP_499732.1| F56A8.4 [Caenorhabditis elegans] 1.0E-150
blast CAE60061 Hypothetical protein CBG03576 [Caenorhabditis briggsae] 6.0E-57
blast CAE60061 Hypothetical protein CBG03576 [Caenorhabditis briggsae] 7.0E-51
blast CAB05744 Hypothetical protein F56A8.4 [Caenorhabditis elegans] ref|NP_499732.1| F56A8.4 [Caenorhabditis elegans] 2.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 6.5E-6
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.3E-4
Sequence
Target Sequence 
>C. ELEGANS:178876_AT
gaaggcggctgaatgggtgactgaattgataagatttccgatttcaaatgttcatatctg
gggacctgatttcgaaggatctgctgatcttttcgattggtcggctatacaacaatgtga
gggcatttcgatgaacctaatctctacgatgaatggaaatttggaaaacttcaaagatct
ggaacaccttgaactcagtggctgttcatggttcagagcggagcattttcgagcattgga
ggcaaatcgtgtttctctttattgcttaaacttcactgttcaagacgtgaatactattct
cagagattggacccgaggatcaaatgcgaaattggaaaatttgattgttcattcgaaaga
gattgatggaagagttccagggcaaattctggaaggtctcgatgtgaaaccatggaacca
cgaacaacggcagaaaattttcccaaaagaagaaccattccaatcattaaactgcgagaa
agcgatggattttgaacgagccgatggtcttctcgcttctgtgatcattaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGCGGCTGAATGGGTGACTGAA 408 333 327 Antisense
GGACCTGATTTCGAAGGATCTGCTG 599 523 388 Antisense
GATCTGCTGATCTTTTCGATTGGTC 261 419 404 Antisense
GGGCATTTCGATGAACCTAATCTCT 402 491 447 Antisense
GAACACCTTGAACTCAGTGGCTGTT 577 351 508 Antisense
GTGGCTGTTCATGGTTCAGAGCGGA 602 491 524 Antisense
GAGCGGAGCATTTTCGAGCATTGGA 330 387 542 Antisense
GGAGGCAAATCGTGTTTCTCTTTAT 262 515 564 Antisense
GAACCATTCCAATCATTAAACTGCG 674 345 778 Antisense
GCGATGGATTTTGAACGAGCCGATG 251 291 808 Antisense
TCTTCTCGCTTCTGTGATCATTAAC 288 617 834 Antisense
Sequence Source Affymetrix Proprietary Database