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[178872_at]

Description C25F9.6 /REP_DB=WormBase Gene ID /WP=CE17470 /TR=O62075 /GB=CAB03919.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 25      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 59.3
(-3)
A 		111.1
(2.6)
A 		97
(1.1)
A 		114.9
(3)
A 		56
B 19.7
(-3)
A 		111.2
(3)
A 		62.9
(-0.2)
A 		98.4
(2.2)
A 		92
C 60
(-3)
A 		90.7
(-0.5)
A 		87.4
(-0.8)
A 		133.4
(3)
A 		73
avr 46.3
(-3) 		104.3
(2) 		82.4
(0.1) 		115.6
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 126
(3)
A 		97.8
(0.9)
A 		44.5
(-3)
A 		106.1
(1.5)
A 		72
(-1)
A 		83.7
(-0.1)
A 		82
B 45.3
(-1.3)
A 		97.7
(3)
A 		73.4
(1)
A 		76.1
(1.2)
A 		89.5
(2.3)
A 		24.5
(-3)
A 		73
C 64.8
(-0.8)
A 		119.2
(2.3)
A 		26.5
(-3)
A 		131.7
(3)
A 		40.9
(-2.2)
A 		77.3
(-0.1)
A 		105
avr 78.7
(0.2) 		104.9
(3) 		48.1
(-3) 		104.6
(3) 		67.5
(-1) 		61.8
(-1.6) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 21.6
(0.4)
A 		24.1
(2.2)
A 		25.3
(3)
A 		16.8
(-3)
A 		9
B 6.3
(-3)
A 		27.5
(3)
A 		14.6
(-0.7)
A 		21
(1.2)
A 		21
C 19.8
(-3)
A 		26.1
(0.5)
A 		30.5
(3)
A 		22.5
(-1.5)
A 		11
avr 15.9
(-3) 		25.9
(3) 		23.5
(1.6) 		20.1
(-0.5) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 23.2
(0.2)
A 		34.8
(3)
A 		9.7
(-3)
A 		26.9
(1.1)
A 		21.7
(-0.1)
A 		20.4
(-0.4)
A 		25
B 15.8
(-1.5)
A 		32.5
(2.1)
A 		17.8
(-1.1)
A 		20.2
(-0.6)
A 		36.4
(3)
A 		9.1
(-3)
A 		27
C 24
(0.5)
A 		36.4
(3)
A 		6.7
(-3)
A 		26.5
(1)
A 		13.2
(-1.7)
A 		25.9
(0.9)
A 		30
avr 21
(-0.5) 		34.6
(3) 		11.4
(-3) 		24.5
(0.4) 		23.8
(0.2) 		18.5
(-1.2) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178872_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13595
Sequence Type Exemplar sequence
Representative Public ID C25F9.6 NCBI
Target Description C25F9.6 /REP_DB=WormBase Gene ID /WP=CE17470 /TR=O62075 /GB=CAB03919.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075375(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075375 NCBI Caenorhabditis elegans C25F9.6 (C25F9.6) mRNA, complete cds. 11/11 None
GENEFINDER00000028327 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19405192:19413172:-1 9/11 None
C25F9.6 ENSEMBL cdna:known chromosome:CEL140:V:19403485:19408072:-1 gene:C25F9.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028312 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19403484-19408072(-) 97.68 100.0
Public Domain and Genome References
Gene Title
Ensembl C25F9.6
Entrez Gene 182906 Entrez gene
SwissProt O62075 EMBL-EBI
WormBase CE17470 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03923 Hypothetical protein C25F9.5 [Caenorhabditis elegans] ref|NP_507777.1| C25F9.5 [Caenorhabditis elegans] 0.0
blast CAD54148 Hypothetical protein M04C3.1b [Caenorhabditis elegans] emb|CAD54124.1| Hypothetical protein M04C3.1b [Caenorhabditis elegans] ref|NP_872172.1| M04C3.1b [Caenorhabditis elegans] 0.0
blast CAB07268 Hypothetical protein M04C3.1a [Caenorhabditis elegans] emb|CAB03924.1| Hypothetical protein M04C3.1a [Caenorhabditis elegans] ref|NP_507782.1| M04C3.1a [Caenorhabditis elegans] 0.0
blast CAB03919 Hypothetical protein C25F9.6 [Caenorhabditis elegans] ref|NP_507776.1| C25F9.6 [Caenorhabditis elegans] 1.0E-144
blast CAB03920 Hypothetical protein C25F9.7 [Caenorhabditis elegans] ref|NP_507775.1| Serpentine Receptor, class W family member (srw-86) [Caenorhabditis elegans] 2.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 1.3E-5
Sequence
Target Sequence 
>C. ELEGANS:178872_AT
gacgtggtgaactattgtgcggcaaaccttgcgactcttcaacgtagaaaatctagggtg
gaaacatttttgaaaaatgcagaggaaaaactttgtgagcaagaaaattttagggttcat
tatgacaaaccggaagcggtggtacccttttaccttgccgaccaactaccttgcctccct
gacacttacagtgcagcgggcgtgccggggcggaaaataatgaaactcacaccagacggc
atggttcgtaattttgccaatttggaagcagcaagagattggattaggatgcgaatcgaa
gagcttgaagatgaaatttctttcagcatgctttcgcaagcaagttgctccagaccggcc
cgtaccacggcagaaagcagttttaatgaagctacggtgaacagttacgcgtcaagtact
gcggctcttcagcgtgaaaaatctaaacttgaaagaattgtggcactcgtagaatatggc
aagtttcagataaaccgaaagcaaacctttgacctagccaaccaactgccatctgcctcc
ctcgggacaatcacgcagcaccgggcaagttacgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGTGGTGAACTATTGTGCGGCAA 468 377 265 Antisense
TATTGTGCGGCAAACCTTGCGACTC 429 663 277 Antisense
CCTCCCTGACACTTACAGTGCAGCG 296 265 438 Antisense
AACTCACACCAGACGGCATGGTTCG 671 139 488 Antisense
TTTCTTTCAGCATGCTTTCGCAAGC 50 673 581 Antisense
CGCAAGCAAGTTGCTCCAGACCGGC 171 255 599 Antisense
GGCCCGTACCACGGCAGAAAGCAGT 512 541 621 Antisense
GTTACGCGTCAAGTACTGCGGCTCT 617 445 668 Antisense
TACTGCGGCTCTTCAGCGTGAAAAA 295 647 681 Antisense
GAAAGCAAACCTTTGACCTAGCCAA 217 355 761 Antisense
TCACGCAGCACCGGGCAAGTTACGG 649 621 815 Antisense
Sequence Source Affymetrix Proprietary Database