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[178867_at]

Description F28B1.4 /REP_DB=WormBase Gene ID /WP=CE15916 /TR=O45414 /GB=CAB04211.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 02      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 8.1
(0.6)
A 		2.3
(-3)
A 		3.3
(-2.4)
A 		11.9
(3)
A 		10
B 1.7
(-3)
A 		2.5
(-2.8)
A 		2.3
(-2.8)
A 		23.1
(3)
A 		21
C 1.6
(-3)
A 		4
(-2.6)
A 		3.9
(-2.6)
A 		37.2
(3)
A 		36
avr 3.8
(-2.7) 		2.9
(-3) 		3.2
(-2.9) 		24.1
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 4.1
(1.8)
A 		1.6
(-3)
A 		2.5
(-1.3)
A 		1.9
(-2.4)
A 		2
(-2.2)
A 		4.7
(3)
A 		3
B 1.4
(-2.7)
A 		1.9
(-1.1)
A 		3.2
(3)
A 		3
(2.4)
A 		2.2
(-0.2)
A 		1.3
(-3)
A 		2
C 1.6
(-3)
A 		4.6
(-0.4)
A 		2.8
(-2)
A 		1.9
(-2.7)
A 		1.6
(-3)
A 		8.6
(3)
A 		7
avr 2.4
(-2) 		2.7
(-1.4) 		2.8
(-1.2) 		2.3
(-2.2) 		1.9
(-3) 		4.9
(3) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 2.9
(3)
A 		0.5
(-3)
A 		0.9
(-2)
A 		1.7
(0)
A 		2
B 0.5
(-3)
A 		0.6
(-2.9)
A 		0.5
(-3)
A 		4.9
(3)
A 		4
C 0.5
(-3)
A 		1.2
(-2.3)
A 		1.3
(-2.2)
A 		6.3
(3)
A 		6
avr 1.3
(-2.1) 		0.8
(-3) 		0.9
(-2.8) 		4.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 0.8
(0)
A 		0.6
(-2)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.6
(-2)
A 		1.1
(3)
A 		1
B 0.5
(-3)
A 		0.6
(-1.5)
A 		0.8
(1.5)
A 		0.8
(1.5)
A 		0.9
(3)
A 		0.5
(-3)
A 		0
C 0.6
(-2.5)
A 		1.4
(-0.6)
A 		0.7
(-2.3)
A 		0.4
(-3)
A 		0.5
(-2.8)
A 		2.9
(3)
A 		3
avr 0.6
(-3) 		0.9
(-1) 		0.7
(-2.3) 		0.6
(-3) 		0.7
(-2.3) 		1.5
(3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178867_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14196
Sequence Type Exemplar sequence
Representative Public ID F28B1.4 NCBI
Target Description F28B1.4 /REP_DB=WormBase Gene ID /WP=CE15916 /TR=O45414 /GB=CAB04211.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074918(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074918 NCBI Caenorhabditis elegans F28B1.4 (F28B1.4) mRNA, complete cds. 11/11 None
GENEFINDER00000006335 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17054588:17062497:1 10/11 None
F28B1.4 ENSEMBL cdna:known chromosome:CEL140:V:17058009:17059662:1 gene:F28B1.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006328 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17058008-17059662(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28B1.4
Entrez Gene 185041 Entrez gene
SwissProt O45414 EMBL-EBI
WormBase CE15916 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04211 Hypothetical protein F28B1.4 [Caenorhabditis elegans] ref|NP_507319.1| F28B1.4 [Caenorhabditis elegans] 0.0
blast CAB04212 Hypothetical protein F28B1.5 [Caenorhabditis elegans] ref|NP_507320.1| F28B1.5 [Caenorhabditis elegans] 0.0
blast CAB04210 Hypothetical protein F28B1.3 [Caenorhabditis elegans] ref|NP_507318.1| F28B1.3 [Caenorhabditis elegans] 0.0
blast CAB04211 Hypothetical protein F28B1.4 [Caenorhabditis elegans] ref|NP_507319.1| F28B1.4 [Caenorhabditis elegans] 1.0E-172
blast CAB04210 Hypothetical protein F28B1.3 [Caenorhabditis elegans] ref|NP_507318.1| F28B1.3 [Caenorhabditis elegans] 1.0E-120
blast CAB04212 Hypothetical protein F28B1.5 [Caenorhabditis elegans] ref|NP_507320.1| F28B1.5 [Caenorhabditis elegans] 3.0E-66
Sequence
Target Sequence 
>C. ELEGANS:178867_AT
gaatgggtgcaagtgtccgcagttgaaatacgtcgatcaggatgggatcgggaatttggg
cgagcagtcggcctatttgggcaatagacagattttcaagccgaaagtgaccgtgggcgg
aaactgtgaagtgatggtagatgaaaccggtcttgaacttctggatcattatttgatgat
cttccgtgaagaagcgcgtccagtttttgtgcgtcgattcctgaatgggactcagatgtc
tcaaatcccactgactgacttggtttgcaagcaacatgacgacaaatatgtgtggttcta
cgaagagaataaactttcggaaaactcgctgtacatcgctctccagagcaatgaaggagc
atgtgaatgtccgaaaattataccttaccgagatgtagtcatagaagagatactgggaac
ttgtgatatactccagtgctctgttaatagttcaattgaatttacgagatttttaacgta
tatacagttcggtaacatactgggatttgatcaattgatgcacgacgttgccattgctca
atgccaaccatatccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGGGTGCAAGTGTCCGCAGTTG 50 333 468 Antisense
GTGTCCGCAGTTGAAATACGTCGAT 446 487 480 Antisense
ATTTGGGCGAGCAGTCGGCCTATTT 131 17 521 Antisense
GACCGTGGGCGGAAACTGTGAAGTG 297 383 576 Antisense
GAAACCGGTCTTGAACTTCTGGATC 154 351 610 Antisense
GAAGCGCGTCCAGTTTTTGTGCGTC 130 345 658 Antisense
GTGCGTCGATTCCTGAATGGGACTC 90 479 676 Antisense
TGTCCGAAAATTATACCTTACCGAG 493 483 835 Antisense
GTGATATACTCCAGTGCTCTGTTAA 328 485 890 Antisense
TGATGCACGACGTTGCCATTGCTCA 159 567 983 Antisense
CATTGCTCAATGCCAACCATATCCG 235 217 999 Antisense
Sequence Source Affymetrix Proprietary Database