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[178858_at]

Description R05D7.3 /REP_DB=WormBase Gene ID /WP=CE16297 /TR=Q9XVK7 /GB=CAB03217.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 345.6
(3)
P 		237.8
(-1)
P 		183.7
(-3)
P 		336.2
(2.7)
P 		162
B 363.8
(3)
P 		223.1
(-2.2)
P 		202.8
(-3)
P 		346.2
(2.3)
P 		161
C 346.5
(3)
P 		281.5
(0.9)
P 		162.8
(-3)
P 		316.8
(2)
P 		184
avr 352
(3) 		247.5
(-0.7) 		183.1
(-3) 		333.1
(2.3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 481.7
(3)
P 		304.1
(-0.2)
P 		174.3
(-2.6)
P 		203.2
(-2)
P 		211.6
(-1.9)
P 		150.5
(-3)
P 		331
B 317.4
(3)
P 		283.1
(1.7)
P 		237
(-0.1)
P 		203.2
(-1.4)
P 		201.4
(-1.5)
P 		161.7
(-3)
P 		156
C 364
(3)
P 		267.3
(-0.1)
P 		239.8
(-0.9)
P 		261.9
(-0.2)
P 		195
(-2.3)
P 		173.9
(-3)
P 		190
avr 387.7
(3) 		284.8
(0.3) 		217
(-1.5) 		222.8
(-1.4) 		202.7
(-1.9) 		162
(-3) 		226
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 125.9
(3)
P 		51.6
(-2.7)
P 		48
(-3)
P 		49.2
(-2.9)
P 		78
B 116
(3)
P 		55.1
(-2.3)
P 		47
(-3)
P 		73.8
(-0.7)
P 		69
C 114.7
(3)
P 		80.9
(-0.3)
P 		56.7
(-2.7)
P 		53.4
(-3)
P 		61
avr 118.9
(3) 		62.5
(-2) 		50.6
(-3) 		58.8
(-2.3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 88.8
(1.4)
P 		108.1
(3)
P 		38
(-2.9)
P 		51.5
(-1.8)
P 		63.9
(-0.7)
P 		36.7
(-3)
P 		71
B 110.4
(3)
P 		94.2
(1.3)
P 		57.5
(-2.6)
P 		53.8
(-3)
P 		81.8
(-0)
P 		60.3
(-2.3)
P 		57
C 134.9
(3)
P 		81.6
(-0.9)
P 		61
(-2.4)
P 		52.7
(-3)
P 		63.1
(-2.2)
P 		58.2
(-2.6)
P 		82
avr 111.4
(3) 		94.6
(1.3) 		52.2
(-2.9) 		52.7
(-2.9) 		69.6
(-1.2) 		51.7
(-3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178858_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2366
Sequence Type Exemplar sequence
Representative Public ID R05D7.3 NCBI
Target Description R05D7.3 /REP_DB=WormBase Gene ID /WP=CE16297 /TR=Q9XVK7 /GB=CAB03217.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060675 NCBI Caenorhabditis elegans R05D7.3 (R05D7.3) mRNA, complete cds. 11/11 None
GENEFINDER00000020582 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12176479:12185860:-1 11/11 None
R05D7.3 ENSEMBL cdna:known chromosome:CEL140:I:12176360:12178578:-1 gene:R05D7.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020574 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12176409-12178509(-) 93.48 100.0
Public Domain and Genome References
Gene Title
Ensembl R05D7.3
Entrez Gene 173095 Entrez gene
SwissProt Q9XVK7 EMBL-EBI
WormBase CE16297 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03217 Hypothetical protein R05D7.3 [Caenorhabditis elegans] ref|NP_493076.1| R05D7.3 [Caenorhabditis elegans] 1.0E-149
blast CAB03217 Hypothetical protein R05D7.3 [Caenorhabditis elegans] ref|NP_493076.1| R05D7.3 [Caenorhabditis elegans] 1.0E-124
blast CAE56758 Hypothetical protein CBG24559 [Caenorhabditis briggsae] 1.0E-94
blast AAF47425 CG12502-PA [Drosophila melanogaster] ref|NP_612067.1| CG12502-PA [Drosophila melanogaster] 4.0E-11
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493076.1 2 66-88,122-144
R05D7.3 2 66-88,122-144
GENEFINDER00000020582 2 58-80,114-136
Sequence
Target Sequence 
>C. ELEGANS:178858_AT
agagtatgcagtacctcggtccaatcctcatgggaatcggctcatttatcctcataatcg
cctgcgtagtaaccctggagagccgtgacaaacatgcacaaatcatcacagaagagtcga
ttcttcagaagcgacgacgattaatatcggaagaagaggagaaagccgagcataatgatg
tttttattgatgatgatgagggaaaggaatcattttttggagatggaagaagatttgtca
ccctggatgccaatcgtctggccccacttgctgacgtggattccctgacggacatctcat
tgacacatcggggaggatcagagtctaggctgcaatccgacgacgctaccgtaccaccac
cggatgatcaatttttccaagaatccgagcttcaagaaattgagcaaaacgatgcgacaa
cctccggactcgttgatttgagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGTATGCAGTACCTCGGTCCAAT 206 69 368 Antisense
CGGTCCAATCCTCATGGGAATCGGC 353 243 384 Antisense
GGGAATCGGCTCATTTATCCTCATA 438 493 399 Antisense
TTATCCTCATAATCGCCTGCGTAGT 148 681 413 Antisense
GTAGTAACCCTGGAGAGCCGTGACA 631 453 433 Antisense
ATTTGTCACCCTGGATGCCAATCGT 184 19 600 Antisense
TGGATTCCCTGACGGACATCTCATT 501 551 644 Antisense
CAGAGTCTAGGCTGCAATCCGACGA 151 207 686 Antisense
TACCGTACCACCACCGGATGATCAA 352 649 714 Antisense
AGCAAAACGATGCGACAACCTCCGG 303 75 770 Antisense
AACCTCCGGACTCGTTGATTTGAGC 392 141 786 Antisense
Sequence Source Affymetrix Proprietary Database