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[178854_at]

Description M05B5.2 /REP_DB=WormBase Gene ID /WP=CE06188 /TR=Q21516 /GB=CAA95834.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 49      4grp_highest_8_8 > 31      4grp_high_8_8 > 20      6grp_total > 48      4grp_total > 20      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 337.3
(-0.2)
P 		339.1
(-0.2)
P 		225.2
(-3)
P 		465.7
(3)
P 		241
B 324.6
(-0.5)
P 		361
(1.2)
P 		269.2
(-3)
P 		401.9
(3)
P 		133
C 346.1
(-0.5)
P 		322.1
(-1.1)
P 		241.1
(-3)
P 		497.2
(3)
P 		256
avr 336
(-0.4) 		340.7
(-0.3) 		245.2
(-3) 		454.9
(3) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 488.3
(3)
P 		277
(-2.1)
P 		305.9
(-1.4)
P 		241.6
(-3)
P 		384.3
(0.5)
P 		288.8
(-1.9)
P 		247
B 368.8
(3)
P 		304.4
(-0.8)
P 		265.8
(-3)
M 		353.3
(2.1)
P 		325
(0.4)
P 		364.9
(2.8)
P 		103
C 275.2
(-1.7)
P 		401.9
(1.2)
P 		221.3
(-3)
P 		401.5
(1.2)
P 		275.8
(-1.7)
P 		476.3
(3)
P 		255
avr 377.4
(3) 		327.8
(0.4) 		264.3
(-3) 		332.1
(0.6) 		328.4
(0.4) 		376.7
(3) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 122.8
(3)
P 		73.6
(-1.6)
P 		58.9
(-3)
P 		68.2
(-2.1)
P 		64
B 103.5
(3)
P 		89.2
(0.9)
P 		62.4
(-3)
P 		85.7
(0.4)
P 		41
C 114.6
(3)
P 		92.6
(-1.3)
P 		84
(-3)
P 		83.8
(-3)
P 		31
avr 113.6
(3) 		85.1
(-0.8) 		68.4
(-3) 		79.2
(-1.6) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 90
(0.1)
P 		98.5
(1.1)
P 		66.6
(-2.4)
P 		61.3
(-3)
P 		116
(3)
P 		70.3
(-2)
P 		55
B 128.3
(2.3)
P 		101.3
(0.1)
P 		64.5
(-3)
M 		93.6
(-0.6)
P 		132.1
(2.7)
P 		136.1
(3)
P 		72
C 102
(-0.3)
P 		122.6
(0.9)
P 		56.3
(-3)
P 		80.7
(-1.6)
P 		89.3
(-1.1)
P 		159.5
(3)
P 		103
avr 106.8
(1.5) 		107.5
(1.5) 		62.5
(-3) 		78.5
(-1.4) 		112.5
(2) 		122
(3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178854_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1864
Sequence Type Exemplar sequence
Representative Public ID M05B5.2 NCBI
Target Description M05B5.2 /REP_DB=WormBase Gene ID /WP=CE06188 /TR=Q21516 /GB=CAA95834.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059628
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000017374 ENSEMBL cdna:SNAP chromosome:CEL140:I:7181084:7182247:-1 None
GENEFINDER00000017396 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7181084:7182247:-1 None
NM_059628 NCBI Caenorhabditis elegans M05B5.2 (M05B5.2) mRNA, complete cds. None
M05B5.2 ENSEMBL cdna:known chromosome:CEL140:I:7180607:7182255:-1 gene:M05B5.2 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7181221-7182178(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M05B5.2
Entrez Gene 187451 Entrez gene
SwissProt Q21516 EMBL-EBI
WormBase CE31044 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA95834 Hypothetical protein M05B5.2 [Caenorhabditis elegans] ref|NP_492029.2| M05B5.2 [Caenorhabditis elegans] 2.0E-68
blast CAE74240 Hypothetical protein CBG21925 [Caenorhabditis briggsae] 1.0E-50
blast AAK68352 Hypothetical protein F28B4.4 [Caenorhabditis elegans] ref|NP_508549.1| F28B4.4 [Caenorhabditis elegans] 3.0E-13
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492029.2 2 7-26,84-106
M05B5.2 2 7-26,84-106
SNAP00000017374 2 7-26,84-106
GENEFINDER00000017396 2 7-26,84-106
Sequence
Target Sequence 
>C. ELEGANS:178854_AT
ctaaggcacttggcttttgccgctgttctctgtacactttgtgagtttttccaaaaattt
aacgcgtcgctgaacatcccaaaaattgaaacattttttaagaaaaaaaaactaaatttc
atcaaaatcttaagctttgggaacttgtccgtttcactcttagccgcaaatatttcggta
gtttatagtggtttttttctcggaagctactatgccaatggacaaggactcaatgaccca
attactcaatatatcgaaggaaggttaggaaatcgacgtattttctggtgtccaagtggt
tatggatatcttgcattctgtcctcaaccaacagattgggataactacaattggtgttgt
acattcccatatatgggatcatggaaaccctcatgctgtcaatttgccattcctactgga
gctgtcgtggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTAAGGCACTTGGCTTTTGCCGCTG 176 227 25 Antisense
CGCTGTTCTCTGTACACTTTGTGAG 502 255 45 Antisense
ATTTAACGCGTCGCTGAACATCCCA 159 13 81 Antisense
GCTTTGGGAACTTGTCCGTTTCACT 259 303 158 Antisense
GTTTCACTCTTAGCCGCAAATATTT 543 449 175 Antisense
TTTTCTCGGAAGCTACTATGCCAAT 489 657 219 Antisense
AATCGACGTATTTTCTGGTGTCCAA 155 169 295 Antisense
TGGATATCTTGCATTCTGTCCTCAA 110 551 327 Antisense
GGATCATGGAAACCCTCATGCTGTC 666 511 400 Antisense
ATGCTGTCAATTTGCCATTCCTACT 247 43 417 Antisense
CATTCCTACTGGAGCTGTCGTGGCT 493 217 432 Antisense
Sequence Source Affymetrix Proprietary Database