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[178845_at]

Description F43G9.8 /REP_DB=WormBase Gene ID /WP=CE10368 /TR=Q93720 /GB=CAB02110.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 18      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 5
(-1.6)
A 		2.2
(-3)
A 		3.3
(-2.5)
A 		14.6
(3)
A 		12
B 2.4
(-3)
A 		5.2
(-1.6)
A 		5.5
(-1.4)
A 		14.1
(3)
A 		12
C 10.9
(-3)
A 		26.7
(-0.6)
A 		15
(-2.4)
A 		50.8
(3)
A 		40
avr 6.1
(-3) 		11.4
(-1.4) 		7.9
(-2.5) 		26.5
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 7.3
(-2.5)
A 		2.8
(-3)
A 		8.4
(-2.4)
A 		14.8
(-1.7)
A 		20.8
(-1)
A 		58.1
(3)
P 		55
B 14.4
(-1.4)
A 		3.3
(-3)
A 		9.9
(-2)
A 		4.1
(-2.9)
A 		31.5
(1.1)
A 		44.7
(3)
P 		41
C 3.1
(-3)
A 		13
(-1.5)
A 		4.6
(-2.8)
A 		17.4
(-0.8)
A 		42.9
(3)
A 		40.7
(2.7)
P 		40
avr 8.3
(-2.7) 		6.4
(-3) 		7.6
(-2.8) 		12.1
(-2.2) 		31.7
(0.7) 		47.8
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 1.8
(1.9)
A 		0.5
(-3)
A 		0.9
(-1.5)
A 		2.1
(3)
A 		2
B 0.8
(-3)
A 		1.3
(-1.6)
A 		1.3
(-1.6)
A 		3
(3)
A 		2
C 3.6
(-3)
A 		7.7
(1.9)
A 		5.2
(-1.1)
A 		8.6
(3)
A 		5
avr 2.1
(-3) 		3.2
(-0.4) 		2.5
(-2) 		4.6
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 1.3
(-2.9)
A 		1
(-3)
A 		1.8
(-2.6)
A 		3.8
(-1.7)
A 		6.3
(-0.6)
A 		14.2
(3)
P 		13
B 5
(-1.5)
A 		1.1
(-3)
A 		2.4
(-2.5)
A 		1.1
(-3)
A 		12.8
(1.5)
A 		16.7
(3)
P 		16
C 1.1
(-3)
A 		4
(-1.6)
A 		1.2
(-3)
A 		3.5
(-1.9)
A 		13.9
(3)
A 		13.6
(2.9)
P 		13
avr 2.5
(-2.7) 		2
(-2.9) 		1.8
(-3) 		2.8
(-2.5) 		11
(1.2) 		14.8
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178845_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2520
Sequence Type Exemplar sequence
Representative Public ID F43G9.8 NCBI
Target Description F43G9.8 /REP_DB=WormBase Gene ID /WP=CE10368 /TR=Q93720 /GB=CAB02110.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059934(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059934 NCBI Caenorhabditis elegans F43G9.8 (F43G9.8) mRNA, complete cds. 11/11 None
SNAP00000043539 ENSEMBL cdna:SNAP chromosome:CEL140:I:8625525:8627014:1 11/11 None
GENEFINDER00000043553 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8625525:8630837:1 11/11 None
F43G9.8 ENSEMBL cdna:known chromosome:CEL140:I:8625525:8627014:1 gene:F43G9.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043540 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8625455-8626945(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F43G9.8
Entrez Gene 185717 Entrez gene
SwissProt Q93720 EMBL-EBI
WormBase CE34308 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC72298 reverse transcriptase [Caenorhabditis elegans] 0.0
blast AAC48148 Hypothetical protein F20B4.7 [Caenorhabditis elegans] ref|NP_510856.1| F20B4.7 [Caenorhabditis elegans] 0.0
blast C87861 protein F43G9.7 [imported] - Caenorhabditis elegans 0.0
blast CAB02110 Hypothetical protein F43G9.8 [Caenorhabditis elegans] ref|NP_492335.2| F43G9.8 [Caenorhabditis elegans] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 3.1E-33
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 3.6E-61
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492335.2 1 47-69
F43G9.8 1 47-69
SNAP00000043539 1 47-69
GENEFINDER00000043553 1 48-70
Sequence
Target Sequence 
>C. ELEGANS:178845_AT
ggagcatacatcatggcatcgttcattttgcaaatactttctgtgattctcttgatgaaa
ataattcgtatatacatgtcgatacacaggaagaatatgagaaagaagaagatgatgaag
ttacaacaaacgaagcaagaagatcctccgtcggcagttttaccatctccgtcccacttt
acaacacaaacttctacaaatactgttgaagatgcgaatatggtccttgaaggcaatgaa
atgctcatgaaacaccgagcgcgacttctaaaaccaacccaaaagagaactacaaaggat
ggcaattctgaaacaccctcaacacatgacggaaccagaaaaatcgtgtttcaagagaaa
ggacaaattcttctaaacaccaacaatgttactgaatatgctgtgatggacggagaagac
aaaccgggcgagaagataaacatcaaaaaagttacatttgattttggatcgtttgttact
tacaatgtagggattgacgtggatgaatgggaagagccaattgagaagattgcgggctcc
gcaggttcgggtggatcagttatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCATACATCATGGCATCGTTCA 433 519 199 Antisense
GGCATCGTTCATTTTGCAAATACTT 379 537 213 Antisense
GAAGATCCTCCGTCGGCAGTTTTAC 472 337 337 Antisense
GTCCCACTTTACAACACAAACTTCT 667 475 369 Antisense
AGATGCGAATATGGTCCTTGAAGGC 550 65 408 Antisense
GGATGGCAATTCTGAAACACCCTCA 222 513 495 Antisense
CACCCTCAACACATGACGGAACCAG 225 201 512 Antisense
GGACAAATTCTTCTAAACACCAACA 22 527 559 Antisense
GATTTTGGATCGTTTGTTACTTACA 331 429 658 Antisense
GAAGATTGCGGGCTCCGCAGGTTCG 693 335 723 Antisense
CGCAGGTTCGGGTGGATCAGTTATG 10 243 738 Antisense
Sequence Source Affymetrix Proprietary Database