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[178841_at]

Description T22G5.3 /REP_DB=WormBase Gene ID /WP=CE13986 /TR=Q9XVI8 /GB=CAB03388.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 30      4grp_total > 21      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 152.1
(3)
P 		71.7
(-2.9)
P 		69.8
(-3)
P 		138.4
(2)
P 		82
B 105.5
(0.8)
P 		84.5
(-0.9)
P 		58.6
(-3)
P 		133.2
(3)
P 		75
C 102.9
(-1.6)
P 		97
(-2)
P 		82.7
(-3)
P 		169.5
(3)
P 		87
avr 120.2
(0.9) 		84.4
(-1.9) 		70.4
(-3) 		147
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 133.4
(1)
P 		166.3
(3)
P 		162.6
(2.8)
P 		133.8
(1)
P 		69.9
(-2.8)
P 		66.6
(-3)
P 		100
B 101.3
(-2.5)
P 		197.8
(3)
P 		130.5
(-0.8)
P 		129.5
(-0.9)
P 		139.6
(-0.3)
P 		91.7
(-3)
P 		106
C 161.8
(2.1)
P 		148.3
(1.4)
P 		107.2
(-0.8)
P 		178.4
(3)
P 		67
(-3)
P 		73.5
(-2.6)
P 		111
avr 132.2
(0.5) 		170.8
(3) 		133.4
(0.6) 		147.2
(1.5) 		92.2
(-2) 		77.3
(-3) 		94
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 55.4
(3)
P 		15.6
(-3)
P 		18.2
(-2.6)
P 		20.3
(-2.3)
P 		40
B 33.6
(3)
P 		20.9
(-0.8)
P 		13.6
(-3)
P 		28.4
(1.4)
P 		20
C 34.1
(3)
P 		27.9
(-3)
P 		28.8
(-2.1)
P 		28.6
(-2.3)
P 		6
avr 41
(3) 		21.5
(-2.6) 		20.2
(-3) 		25.8
(-1.4) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 24.6
(-1.8)
P 		59.2
(3)
P 		35.4
(-0.3)
P 		33.9
(-0.5)
P 		21.1
(-2.3)
P 		16.2
(-3)
P 		43
B 35.2
(-2.4)
P 		65.8
(3)
P 		31.7
(-3)
P 		34.3
(-2.5)
P 		56.7
(1.4)
P 		34.2
(-2.6)
P 		34
C 60
(3)
P 		45.2
(0.7)
P 		27.3
(-2.1)
P 		35.9
(-0.8)
P 		21.7
(-3)
P 		24.6
(-2.5)
P 		38
avr 39.9
(-0.2) 		56.7
(3) 		31.5
(-1.8) 		34.7
(-1.2) 		33.2
(-1.4) 		25
(-3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178841_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14512
Sequence Type Exemplar sequence
Representative Public ID T22G5.3 NCBI
Target Description T22G5.3 /REP_DB=WormBase Gene ID /WP=CE13986 /TR=Q9XVI8 /GB=CAB03388.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074040(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074040 NCBI Caenorhabditis elegans T22G5.3 (T22G5.3) mRNA, complete cds. 11/11 None
SNAP00000016895 ENSEMBL cdna:SNAP chromosome:CEL140:V:13883505:13886510:1 11/11 None
GENEFINDER00000016904 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13883505:13886979:1 11/11 None
T22G5.3 ENSEMBL cdna:known chromosome:CEL140:V:13883505:13886650:1 gene:T22G5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13883504-13885058(+) 80.73 100.0
Public Domain and Genome References
Gene Title
Ensembl T22G5.3
Entrez Gene 179883 Entrez gene
SwissProt Q9XVI8 EMBL-EBI
WormBase CE28964 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03388 Hypothetical protein T22G5.3 [Caenorhabditis elegans] ref|NP_506441.1| T22G5.3 [Caenorhabditis elegans] 0.0
blast CAB03388 Hypothetical protein T22G5.3 [Caenorhabditis elegans] ref|NP_506441.1| T22G5.3 [Caenorhabditis elegans] 1.0E-172
blast CAE66213 Hypothetical protein CBG11455 [Caenorhabditis briggsae] 8.0E-69
blast CAE66213 Hypothetical protein CBG11455 [Caenorhabditis briggsae] 3.0E-65
blast CAE66213 Hypothetical protein CBG11455 [Caenorhabditis briggsae] 5.0E-65
blast CAB55158 Hypothetical protein Y116A8C.41 [Caenorhabditis elegans] ref|NP_503041.1| Y116A8C.41 [Caenorhabditis elegans] 5.0E-12
blast CAE60204 Hypothetical protein CBG03766 [Caenorhabditis briggsae] 8.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR008627 EMBL-EBI GETHR pentapeptide 3.7E-4
Pfam IPR008627 EMBL-EBI GETHR pentapeptide 3.6E-7
Sequence
Target Sequence 
>C. ELEGANS:178841_AT
gggatagactcaaccaagtcgacgatttgacattcatcacatactttcgtttgacggaca
aagagtttcatctattcaaagtgaaaattcttcccaagaaaaaacttactttaaactttt
tcaactatgaaaaccaaaattcaattcaattggagttcaatcatgataatcttgaaatat
gttctgcaccgtggtttactcgacaggatcttttgaatactaactgccaaaatttggtga
ttgataaaaccaaattaactaacaacgatctgaatgcttatatcaaaaaatggttggatg
gagctgatacaaaattggaatccttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGATAGACTCAACCAAGTCGACGA 348 493 482 Antisense
GTCGACGATTTGACATTCATCACAT 34 475 499 Antisense
TGACATTCATCACATACTTTCGTTT 112 571 509 Antisense
CATCACATACTTTCGTTTGACGGAC 313 211 516 Antisense
GACGGACAAAGAGTTTCATCTATTC 292 377 534 Antisense
TTCAAAGTGAAAATTCTTCCCAAGA 226 685 556 Antisense
GAAATATGTTCTGCACCGTGGTTTA 685 361 655 Antisense
ACCGTGGTTTACTCGACAGGATCTT 276 85 669 Antisense
AGGATCTTTTGAATACTAACTGCCA 367 57 686 Antisense
AACTAACAACGATCTGAATGCTTAT 29 139 738 Antisense
GCTGATACAAAATTGGAATCCTTCA 152 297 784 Antisense
Sequence Source Affymetrix Proprietary Database