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[178828_at]

Description K08E4.3 /REP_DB=WormBase Gene ID /WP=CE03463 /TR=Q21334 /GB=CAA92681.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 15      4grp_high_8_8 > 21      6grp_highest_nonorm > 93      6grp_total > 68      4grp_total > 10      6grp_highest_sub > Da      6grp_high1_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 239
1 2 3 4 range
A 279.7
(-2.8)
P 		295.1
(-2.3)
P 		273
(-3)
P 		462.9
(3)
P 		190
B 224.3
(-3)
P 		280.6
(-1.3)
P 		274.8
(-1.5)
P 		420.2
(3)
P 		196
C 259
(-2.7)
P 		300.1
(-0.6)
P 		252.6
(-3)
P 		373.6
(3)
P 		121
avr 254.3
(-3) 		291.9
(-1.6) 		266.8
(-2.5) 		418.9
(3) 		165

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 473
1 2 3 4 5 6 range
A 362.1
(-1.3)
P 		250.4
(-2.8)
P 		239.5
(-3)
P 		342.5
(-1.6)
P 		384.4
(-1)
P 		667.1
(3)
P 		428
B 396.7
(-0.6)
P 		279.1
(-2.2)
P 		216.7
(-3)
P 		283.1
(-2.1)
P 		394
(-0.6)
P 		662.9
(3)
P 		446
C 321.7
(-2.2)
P 		264.2
(-3)
P 		270.1
(-2.9)
P 		301.9
(-2.5)
P 		357.5
(-1.7)
P 		689.7
(3)
P 		426
avr 360.2
(-1.4) 		264.6
(-2.7) 		242.1
(-3) 		309.2
(-2.1) 		378.6
(-1.1) 		673.2
(3) 		431
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 101.8
(3)
P 		64.1
(-3)
P 		71.4
(-1.8)
P 		67.8
(-2.4)
P 		38
B 71.5
(-1.2)
P 		69.3
(-1.7)
P 		63.7
(-3)
P 		89.5
(3)
P 		26
C 85.7
(2.4)
P 		86.3
(2.6)
P 		88.1
(3)
P 		63
(-3)
P 		25
avr 86.3
(3) 		73.2
(-3) 		74.4
(-2.5) 		73.4
(-2.9) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 66.7
(-2.2)
P 		89.1
(-1)
P 		52.2
(-3)
P 		86.9
(-1.1)
P 		116
(0.5)
P 		162.4
(3)
P 		110
B 138
(-0.4)
P 		92.9
(-1.8)
P 		52.6
(-3)
P 		75
(-2.3)
P 		160.1
(0.3)
P 		247.2
(3)
P 		195
C 119.3
(-0.9)
P 		80.6
(-2.3)
P 		68.7
(-2.7)
P 		60.7
(-3)
P 		115.7
(-1.1)
P 		230.9
(3)
P 		170
avr 108
(-1.1) 		87.5
(-1.9) 		57.8
(-3) 		74.2
(-2.4) 		130.6
(-0.2) 		213.5
(3) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178828_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9314
Sequence Type Exemplar sequence
Representative Public ID K08E4.3 NCBI
Target Description K08E4.3 /REP_DB=WormBase Gene ID /WP=CE03463 /TR=Q21334 /GB=CAA92681.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069884(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069884 NCBI Caenorhabditis elegans K08E4.3 (K08E4.3) mRNA, complete cds. 11/11 None
SNAP00000000848 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12057719:12059611:1 11/11 None
GENEFINDER00000000854 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12057719:12059611:1 11/11 None
K08E4.3 ENSEMBL cdna:known chromosome:CEL140:IV:12057509:12059611:1 gene:K08E4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12057514-12059617(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08E4.3
Entrez Gene 187159 Entrez gene
SwissProt Q21334 EMBL-EBI
WormBase CE03463 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92681 Hypothetical protein K08E4.3 [Caenorhabditis elegans] ref|NP_502285.1| K08E4.3 [Caenorhabditis elegans] 0.0
blast CAA92681 Hypothetical protein K08E4.3 [Caenorhabditis elegans] ref|NP_502285.1| K08E4.3 [Caenorhabditis elegans] 1.0E-163
blast CAE62172 Hypothetical protein CBG06219 [Caenorhabditis briggsae] 1.0E-141
blast CAE62172 Hypothetical protein CBG06219 [Caenorhabditis briggsae] 1.0E-140
blast CAE17754 Hypothetical protein C46C2.6 [Caenorhabditis elegans] ref|NP_001023067.1| C46C2.6 [Caenorhabditis elegans] 2.0E-50
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502285.1 3 46-68,72-91,163-182
K08E4.3 3 46-68,72-91,163-182
Sequence
Target Sequence 
>C. ELEGANS:178828_AT
acggaggcatcgtttcaggacctgtcgccacggctttggttactggaatgattggaatga
ccgcaaagacagtggccgagttgacaagtttcaatccacgtctttccgaaggtggatgtg
aatggtttggaactgcaccactttgcaatttcccgtgtccttctgattatgactacattc
gtgcaaataacggaagatgctcttcttggtggctctccggattctgctctcctgatccaa
gtttcggaaagccatgctccacgattctcggagactattttagtaaacgattctgctgta
aatccgatccaatggagtgtacgtggtctggaagatggatgggtgccaatacagcacata
atatctactgtagatacgacaatgttggaaaatgtggaacaattgattgttctatcaatc
actttactttgaaggcacaaaattcatctgaaattaccggcgatcgttgtgaccgtctcg
agctgtttggtcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGAGGCATCGTTTCAGGACCTGT 110 95 497 Antisense
TCGCCACGGCTTTGGTTACTGGAAT 421 597 521 Antisense
ATGACCGCAAAGACAGTGGCCGAGT 262 45 553 Antisense
GACAAGTTTCAATCCACGTCTTTCC 53 365 579 Antisense
GAACTGCACCACTTTGCAATTTCCC 592 347 626 Antisense
TTTCCCGTGTCCTTCTGATTATGAC 638 669 645 Antisense
TGCTCTCCTGATCCAAGTTTCGGAA 554 581 721 Antisense
TTTCGGAAAGCCATGCTCCACGATT 606 665 738 Antisense
ACGATTCTGCTGTAAATCCGATCCA 95 93 783 Antisense
TTACCGGCGATCGTTGTGACCGTCT 643 681 950 Antisense
GACCGTCTCGAGCTGTTTGGTCTTC 330 383 967 Antisense
Sequence Source Affymetrix Proprietary Database