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[178827_at]

Description ZK218.1 /REP_DB=WormBase Gene ID /WP=CE16701 /TR=O45998 /GB=CAB04984.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 45      4grp_total > 46      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 225
1 2 3 4 range
A 235.1
(3)
P 		54.5
(-2.7)
A 		51.6
(-2.8)
A 		45.2
(-3)
A 		190
B 210.7
(3)
P 		55.8
(-2.3)
A 		60.1
(-2.2)
A 		35.9
(-3)
A 		175
C 245.4
(3)
P 		80.2
(-1.4)
A 		57.4
(-2)
A 		20.6
(-3)
A 		225
avr 230.4
(3) 		63.5
(-2.1) 		56.4
(-2.3) 		33.9
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 5.6
(-2.5)
A 		3.8
(-3)
A 		23.9
(2.9)
A 		4.9
(-2.7)
A 		8.7
(-1.6)
A 		24.4
(3)
A 		21
B 26.9
(0.7)
A 		28.2
(0.9)
A 		39.2
(3)
A 		7.3
(-3)
A 		34.5
(2.1)
A 		26.7
(0.6)
A 		32
C 30.2
(0.5)
A 		49.5
(3)
A 		3.9
(-3)
A 		7
(-2.6)
A 		17.5
(-1.2)
A 		28.6
(0.3)
A 		46
avr 20.9
(1.2) 		27.2
(3) 		22.3
(1.6) 		6.4
(-3) 		20.2
(1) 		26.6
(2.8) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 85.6
(3)
P 		11.8
(-2.6)
A 		13.5
(-2.5)
A 		6.6
(-3)
A 		79
B 67.2
(3)
P 		13.8
(-2.4)
A 		13.9
(-2.4)
A 		7.7
(-3)
A 		60
C 81.2
(3)
P 		23.1
(-1.5)
A 		20
(-1.7)
A 		3.5
(-3)
A 		78
avr 78
(3) 		16.2
(-2.1) 		15.8
(-2.2) 		5.9
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 1
(-3)
A 		1.4
(-2.5)
A 		5.2
(2.1)
A 		1.2
(-2.8)
A 		2.6
(-1)
A 		5.9
(3)
A 		5
B 9.4
(0.7)
A 		9.4
(0.7)
A 		9.5
(0.8)
A 		1.9
(-3)
A 		14
(3)
A 		10
(1)
A 		12
C 11.2
(1.3)
A 		15.1
(3)
A 		1
(-3)
A 		1.4
(-2.8)
A 		5.7
(-1)
A 		9.6
(0.7)
A 		14
avr 7.2
(1.8) 		8.6
(3) 		5.2
(0.1) 		1.5
(-3) 		7.4
(2) 		8.5
(2.9) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178827_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13253
Sequence Type Exemplar sequence
Representative Public ID ZK218.1 NCBI
Target Description ZK218.1 /REP_DB=WormBase Gene ID /WP=CE16701 /TR=O45998 /GB=CAB04984.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074928(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074928 NCBI Caenorhabditis elegans ZK218.1 (ZK218.1) mRNA, complete cds. 11/11 None
SNAP00000041060 ENSEMBL cdna:SNAP chromosome:CEL140:V:17095049:17095979:1 11/11 None
GENEFINDER00000041070 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17095049:17095979:1 11/11 None
ZK218.1 ENSEMBL cdna:known chromosome:CEL140:V:17095148:17095979:1 gene:ZK218.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17095147-17095979(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.1
Entrez Gene 191243 Entrez gene
SwissProt O45998 EMBL-EBI
WormBase CE16701 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04984 Hypothetical protein ZK218.1 [Caenorhabditis elegans] ref|NP_507329.1| ZK218.1 [Caenorhabditis elegans] 1.0E-148
blast CAB04982 Hypothetical protein ZK218.3 [Caenorhabditis elegans] ref|NP_507331.1| ZK218.3 [Caenorhabditis elegans] 1.0E-106
blast CAB04990 Hypothetical protein ZK218.11 [Caenorhabditis elegans] ref|NP_507336.1| ZK218.11 [Caenorhabditis elegans] 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.9E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.9E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000041060 1 23-45
GENEFINDER00000041070 1 23-45
Sequence
Target Sequence 
>C. ELEGANS:178827_AT
tacactccgggagcaactaactgtgttaatacgtattcagataagacgtgtgaatccctt
tacgccatcgcagcggctaatccagcaccagtgccaggtcttaaaactctgcgcccagga
aaatgttttaccacagctgccactgccgctgttacttcacctatcgatgcagatcttgtg
agagcagcagtcagcacttgtccaaagacttgtggatattgttgccaaagtcctgattac
aactgtcctaatgttcagttcccacgtctcaattgcgccaccatccttccatcccaatgc
acaaatcaacaattccgtgtgatcattgctcaagattgtccatctgcctgtgggctctgt
aatgcaggaggatgcgttgatgcgataatgggatgtgcaaatgatatatccatctgtaat
aacgtcggaatgcaagatttcgtcaatctgaactgccaaagaacttgctccagatgctcc
tccgcaccagttccaggaggttgtacctacaatcgtgactctggcactttttgcgccgct
tgggctgccaacggattctgccagaacgaattctactctcctgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACACTCCGGGAGCAACTAACTGTG 488 639 124 Antisense
AGATAAGACGTGTGAATCCCTTTAC 356 61 162 Antisense
GTGCCAGGTCTTAAAACTCTGCGCC 290 477 214 Antisense
AAAATGTTTTACCACAGCTGCCACT 379 133 243 Antisense
GCTGTTACTTCACCTATCGATGCAG 76 295 271 Antisense
TGAGAGCAGCAGTCAGCACTTGTCC 578 567 302 Antisense
AAGTCCTGATTACAACTGTCCTAAT 147 159 351 Antisense
CCATCTGCCTGTGGGCTCTGTAATG 533 271 463 Antisense
TCCGCACCAGTTCCAGGAGGTTGTA 115 597 604 Antisense
AATCGTGACTCTGGCACTTTTTGCG 383 169 634 Antisense
CAGAACGAATTCTACTCTCCTGACC 381 203 685 Antisense
Sequence Source Affymetrix Proprietary Database