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[178815_at]

Description F16H6.3 /REP_DB=WormBase Gene ID /WP=CE18599 /TR=Q9XV90 /GB=CAB04129.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 6.5
(-3)
A 		20.5
(-1.5)
A 		61.7
(3)
A 		9.3
(-2.7)
A 		55
B 38.8
(-0)
A 		63.1
(3)
A 		14.6
(-3)
A 		23.6
(-1.9)
A 		49
C 24.5
(-3)
A 		45.7
(3)
A 		32.3
(-0.8)
A 		25.3
(-2.8)
A 		21
avr 23.3
(-2) 		43.1
(3) 		36.2
(1.3) 		19.4
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 29.9
(-0.2)
A 		58
(3)
A 		5.5
(-3)
A 		41
(1.1)
A 		13.9
(-2)
A 		24.6
(-0.8)
A 		53
B 35.4
(1.7)
A 		11
(-3)
A 		24.8
(-0.3)
A 		33.9
(1.4)
A 		23.4
(-0.6)
A 		42
(3)
A 		31
C 18.1
(-0.5)
A 		14.1
(-1.5)
A 		16.2
(-0.9)
A 		15.3
(-1.2)
A 		32.5
(3)
A 		7.7
(-3)
A 		25
avr 27.8
(2.1) 		27.7
(2) 		15.5
(-3) 		30.1
(3) 		23.3
(0.2) 		24.8
(0.8) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 2.4
(-2.6)
A 		4.4
(-1.8)
A 		16.1
(3)
A 		1.4
(-3)
A 		15
B 12.4
(1.4)
A 		15.6
(3)
A 		3.4
(-3)
A 		5
(-2.2)
A 		12
C 8.1
(-0.4)
A 		13.1
(3)
A 		11.2
(1.7)
A 		4.3
(-3)
A 		9
avr 7.6
(0.2) 		11
(3) 		10.2
(2.4) 		3.6
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 5.5
(-1.7)
A 		20.6
(3)
A 		1.2
(-3)
A 		10.4
(-0.2)
A 		4.2
(-2.1)
A 		6
(-1.5)
A 		19
B 12.3
(1.3)
A 		3.7
(-3)
A 		6
(-1.9)
A 		9
(-0.4)
A 		9.5
(-0.1)
A 		15.7
(3)
A 		12
C 6.7
(0.1)
A 		4.3
(-1.7)
A 		4.1
(-1.9)
A 		3.1
(-2.6)
A 		10.5
(3)
A 		2.6
(-3)
A 		8
avr 8.2
(1.6) 		9.5
(3) 		3.8
(-3) 		7.5
(0.9) 		8.1
(1.5) 		8.1
(1.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178815_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13208
Sequence Type Exemplar sequence
Representative Public ID F16H6.3 NCBI
Target Description F16H6.3 /REP_DB=WormBase Gene ID /WP=CE18599 /TR=Q9XV90 /GB=CAB04129.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075157 NCBI Caenorhabditis elegans F16H6.3 (F16H6.3) mRNA, complete cds. 11/11 None
SNAP00000001296 ENSEMBL cdna:SNAP chromosome:CEL140:V:18194050:18205979:1 11/11 None
F16H6.3 ENSEMBL cdna:known chromosome:CEL140:V:18197880:18200437:1 gene:F16H6.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000001306 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18197879-18200437(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16H6.3
Entrez Gene 184590 Entrez gene
SwissProt Q9XV90 EMBL-EBI
WormBase CE18599 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04128 Hypothetical protein F16H6.1 [Caenorhabditis elegans] ref|NP_507557.1| C-type LECtin family member (clec-42) [Caenorhabditis elegans] 0.0
blast CAB02691 Hypothetical protein B0365.6 [Caenorhabditis elegans] ref|NP_506271.1| C-type LECtin family member (clec-41) [Caenorhabditis elegans] 0.0
blast CAE64660 Hypothetical protein CBG09432 [Caenorhabditis briggsae] 0.0
blast CAB04129 Hypothetical protein F16H6.3 [Caenorhabditis elegans] ref|NP_507558.1| F16H6.3 [Caenorhabditis elegans] 1.0E-138
blast CAB04184 Hypothetical protein F26D2.14 [Caenorhabditis elegans] ref|NP_507156.2| F26D2.14 [Caenorhabditis elegans] 4.0E-79
blast CAB04183 Hypothetical protein F26D2.13 [Caenorhabditis elegans] ref|NP_507155.1| F26D2.13 [Caenorhabditis elegans] 3.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.6E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.6E-15
Pfam IPR001304 EMBL-EBI C-type lectin 3.9E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.6E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.2E-8
Pfam IPR000859 EMBL-EBI CUB 3.2E-9
Pfam IPR000859 EMBL-EBI CUB 1.9E-9
Pfam IPR010381 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF976 1.5E-107
Pfam IPR010381 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF976 8.4E-102
Sequence
Target Sequence 
>C. ELEGANS:178815_AT
gacaatgtccgcacggagaccttctgccgtgttctgttaggtgttgtacctaccgccgga
ggcaacgggctccgtccggtcaggtgctggaccaggggtccagcgccgtatcttactgag
ccaattaatccggatctggttgtggatggcatggcgacgtgcccaaaaacttgcggattc
tgttgcaagaccagtgcgtataactgtaggaatgctgatttcccccgtctcaactgcgcc
acaatcctacctgctcagtgcagagatcaacgctggcgaacgatcatcgcagaggactgc
ccgtcggcttgcgggttctgtaatctcggaggttgtgtggacgcaattgccgactgtccc
gtcgacatatccatctgtaacgctgttggaatgcaagattttgtgaataaaaactgccaa
cggacatgctccagatgcacgaatgctactacgactactcaagcgtcttgtagcagcagt
ggcggtagcaccagcacatgcacctcgtataacccggattcgagttcctcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAATGTCCGCACGGAGACCTTCT 317 365 160 Antisense
CTTCTGCCGTGTTCTGTTAGGTGTT 630 219 180 Antisense
TGGATGGCATGGCGACGTGCCCAAA 212 549 302 Antisense
TGTAGGAATGCTGATTTCCCCCGTC 501 557 364 Antisense
CACAATCCTACCTGCTCAGTGCAGA 19 195 399 Antisense
TGTGGACGCAATTGCCGACTGTCCC 201 555 495 Antisense
GTCGACATATCCATCTGTAACGCTG 617 473 520 Antisense
ACGGACATGCTCCAGATGCACGAAT 550 95 579 Antisense
GCTACTACGACTACTCAAGCGTCTT 232 301 604 Antisense
AGCGTCTTGTAGCAGCAGTGGCGGT 649 81 621 Antisense
TATAACCCGGATTCGAGTTCCTCGT 536 659 667 Antisense
Sequence Source Affymetrix Proprietary Database