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[178814_at]

Description F32B4.5 /REP_DB=WormBase Gene ID /WP=CE17745 /TR=O62200 /GB=CAB04230.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 08      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 9.2
(-3)
A 		12.5
(-2.6)
A 		63.8
(3)
A 		49.7
(1.5)
M 		55
B 66.6
(-1.5)
A 		54.6
(-3)
A 		103.4
(3)
A 		96.1
(2.1)
A 		49
C 64.7
(-0.7)
A 		70.6
(-0.5)
A 		7.4
(-3)
A 		159
(3)
P 		152
avr 46.8
(-2.9) 		45.9
(-3) 		58.2
(-1.7) 		101.6
(3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 32.2
(-3)
A 		41.4
(-1.8)
A 		64.6
(1.1)
A 		35.4
(-2.6)
A 		79.2
(3)
A 		40.7
(-1.9)
A 		47
B 14.6
(-3)
A 		72.4
(1.1)
A 		37.9
(-1.3)
A 		14.4
(-3)
A 		99.7
(3)
P 		43.4
(-1)
P 		85
C 25.2
(-3)
A 		45
(-1.3)
A 		48.7
(-1)
A 		95.3
(3)
A 		46.6
(-1.2)
M 		39.1
(-1.8)
A 		70
avr 24
(-3) 		52.9
(0.4) 		50.4
(0.1) 		48.4
(-0.1) 		75.2
(3) 		41.1
(-1) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 3.4
(-2.7)
A 		2.7
(-3)
A 		16.7
(3)
A 		7.3
(-1)
M 		14
B 21.2
(1.4)
A 		13.5
(-3)
A 		24
(3)
A 		20.5
(1)
A 		11
C 21.4
(1.7)
A 		20.3
(1.4)
A 		2.6
(-3)
A 		26.8
(3)
P 		24
avr 15.3
(0.1) 		12.2
(-3) 		14.4
(-0.8) 		18.2
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 5.9
(-3)
A 		14.7
(-0.1)
A 		14.1
(-0.3)
A 		9
(-2)
A 		23.9
(3)
A 		9.9
(-1.7)
A 		18
B 5.1
(-2.8)
A 		24.1
(0.3)
A 		9.2
(-2.1)
A 		3.8
(-3)
A 		40.5
(3)
P 		16.2
(-1)
P 		37
C 9.3
(-3)
A 		13.7
(-0.3)
A 		12.4
(-1.1)
A 		19.2
(3)
A 		15.1
(0.5)
M 		13.1
(-0.7)
A 		10
avr 6.8
(-3) 		17.5
(0.3) 		11.9
(-1.4) 		10.7
(-1.8) 		26.5
(3) 		13.1
(-1.1) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178814_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2024
Sequence Type Exemplar sequence
Representative Public ID F32B4.5 NCBI
Target Description F32B4.5 /REP_DB=WormBase Gene ID /WP=CE17745 /TR=O62200 /GB=CAB04230.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060543(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060543 NCBI Caenorhabditis elegans F32B4.5 (F32B4.5) mRNA, complete cds. 11/11 None
SNAP00000030035 ENSEMBL cdna:SNAP chromosome:CEL140:I:11527192:11528609:-1 9/11 None
GENEFINDER00000030045 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11527517:11528783:-1 9/11 None
F32B4.5 ENSEMBL cdna:known chromosome:CEL140:I:11527192:11528783:-1 gene:F32B4.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11527122-11528714(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F32B4.5
Entrez Gene 185191 Entrez gene
SwissProt O62200 EMBL-EBI
WormBase CE17745 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04230 Hypothetical protein F32B4.5 [Caenorhabditis elegans] ref|NP_492944.1| F32B4.5 [Caenorhabditis elegans] 1.0E-105
blast CAE60708 Hypothetical protein CBG04376 [Caenorhabditis briggsae] 2.0E-63
blast CAB04230 Hypothetical protein F32B4.5 [Caenorhabditis elegans] ref|NP_492944.1| F32B4.5 [Caenorhabditis elegans] 3.0E-43
blast CAE60708 Hypothetical protein CBG04376 [Caenorhabditis briggsae] 3.0E-38
blast CAB04230 Hypothetical protein F32B4.5 [Caenorhabditis elegans] ref|NP_492944.1| F32B4.5 [Caenorhabditis elegans] 4.0E-34
blast CAE60708 Hypothetical protein CBG04376 [Caenorhabditis briggsae] 6.0E-21
Sequence
Target Sequence 
>C. ELEGANS:178814_AT
cacttcaacgtgctccacaatcgttgctcgccacttcgcccgacgcaacgtctgattcag
atgacaagattgtgcggatcctcgtcgaggcgcttcgtcgcagtgacatgagcattatcg
tctccgaatctttcgatcagtgggcccgattggccgttgaggccaaacgtcttgggctca
tttcatttgtggaggccgcctgcccgtcaaccattagcatctcttgtcatgcggctctct
caaccggacgtatcaatccagaagtcacgtttcgaaaggtacacaatatccattttatgg
ctcaaaaaacaacaggaaaagctttcgcgtcatcaaatcccgaaccagtaaagggaacaa
caacaagcccatttttcctgctctcgtttttttttccttttttctgttcatttctcttgc
ggttttctcccatcaattttcccctattattgcttatgaatacagcaataggggaatatg
ttctacgaatcgtcgtatccggaaaagtaatcatgtgccgagcagtgttcggcgattcgt
tga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTTCAACGTGCTCCACAATCGTT 228 197 245 Antisense
GACAAGATTGTGCGGATCCTCGTCG 638 363 307 Antisense
GCGCTTCGTCGCAGTGACATGAGCA 12 289 334 Antisense
GAGCATTATCGTCTCCGAATCTTTC 298 389 354 Antisense
ATCAGTGGGCCCGATTGGCCGTTGA 76 3 380 Antisense
CAAACGTCTTGGGCTCATTTCATTT 112 193 408 Antisense
TCATTTCATTTGTGGAGGCCGCCTG 382 619 422 Antisense
GTCAACCATTAGCATCTCTTGTCAT 160 465 450 Antisense
GCTTTCGCGTCATCAAATCCCGAAC 641 303 565 Antisense
AATTTTCCCCTATTATTGCTTATGA 352 175 679 Antisense
CGAGCAGTGTTCGGCGATTCGTTGA 233 251 763 Antisense
Sequence Source Affymetrix Proprietary Database