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[178800_at]

Description ZK218.5 /REP_DB=WormBase Gene ID /WP=CE16705 /TR=O46000 /GB=CAB04986.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 18      4grp_total > 52      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 113.4
(1.3)
A 		149
(3)
A 		83.3
(-0.2)
A 		24.7
(-3)
A 		124
B 136.7
(3)
A 		114
(1.8)
A 		101.8
(1.2)
A 		22
(-3)
A 		115
C 66.3
(-3)
A 		96
(1.3)
A 		107.3
(3)
A 		68.2
(-2.7)
A 		41
avr 105.5
(2) 		119.7
(3) 		97.5
(1.4) 		38.3
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 380.2
(3)
M 		93.2
(-1.7)
A 		21.5
(-2.8)
A 		25.8
(-2.8)
A 		13.5
(-3)
A 		11
(-3)
A 		369
B 296.3
(3)
P 		54
(-2)
A 		17.4
(-2.8)
A 		62.5
(-1.9)
A 		32.4
(-2.5)
A 		7.3
(-3)
A 		289
C 310.5
(3)
P 		27.7
(-2.6)
A 		35.6
(-2.5)
A 		10.1
(-3)
A 		8.2
(-3)
A 		9.1
(-3)
A 		302
avr 329
(3) 		58.3
(-2.1) 		24.8
(-2.7) 		32.8
(-2.6) 		18
(-2.8) 		9.1
(-3) 		320
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 41.3
(3)
A 		32.3
(1.6)
A 		21.8
(-0.1)
A 		3.6
(-3)
A 		38
B 43.6
(3)
A 		28.2
(0.6)
A 		23.6
(-0.1)
A 		4.7
(-3)
A 		39
C 21.9
(-0.6)
A 		27.6
(0.7)
A 		37.4
(3)
A 		11.5
(-3)
A 		26
avr 35.6
(3) 		29.4
(1.7) 		27.6
(1.3) 		6.6
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 70.1
(3)
M 		33.1
(-0.3)
A 		4.7
(-2.8)
A 		6.6
(-2.7)
A 		4.1
(-2.9)
A 		2.7
(-3)
A 		67
B 103.1
(3)
P 		18
(-2.1)
A 		4.2
(-2.9)
A 		16.5
(-2.2)
A 		13.2
(-2.4)
A 		2.7
(-3)
A 		100
C 115.1
(3)
P 		8.5
(-2.7)
A 		9.1
(-2.6)
A 		2
(-3)
A 		2.7
(-3)
A 		3
(-2.9)
A 		113
avr 96.1
(3) 		19.9
(-1.9) 		6
(-2.8) 		8.4
(-2.6) 		6.7
(-2.7) 		2.8
(-3) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178800_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13679
Sequence Type Exemplar sequence
Representative Public ID ZK218.5 NCBI
Target Description ZK218.5 /REP_DB=WormBase Gene ID /WP=CE16705 /TR=O46000 /GB=CAB04986.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074932(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074932 NCBI Caenorhabditis elegans ZK218.5 (ZK218.5) mRNA, complete cds. 11/11 None
SNAP00000041064 ENSEMBL cdna:SNAP chromosome:CEL140:V:17103710:17104560:1 11/11 None
GENEFINDER00000041077 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17101997:17104560:1 11/11 None
ZK218.5 ENSEMBL cdna:known chromosome:CEL140:V:17103710:17104560:1 gene:ZK218.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17103709-17104560(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK218.5
Entrez Gene 191245 Entrez gene
SwissProt O46000 EMBL-EBI
WormBase CE16705 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 1.0E-151
blast CAB04986 Hypothetical protein ZK218.5 [Caenorhabditis elegans] ref|NP_507333.1| ZK218.5 [Caenorhabditis elegans] 1.0E-150
blast CAB04982 Hypothetical protein ZK218.3 [Caenorhabditis elegans] ref|NP_507331.1| ZK218.3 [Caenorhabditis elegans] 1.0E-108
blast CAB04987 Hypothetical protein ZK218.7 [Caenorhabditis elegans] ref|NP_507335.1| ZK218.7 [Caenorhabditis elegans] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.9E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000041077 5 41-63,73-92,115-137,152-174,194-216
Sequence
Target Sequence 
>C. ELEGANS:178800_AT
agaagacgcaccagtgcctggagctaaaacagctcgcccattaaaatgctttactgcatt
ggctgctgcaaccgatccggtggaaccagacctagtgaaaactgcagccgcttcatgtcc
aaagacttgtggatattgttgtgaaagtcctgattacaaatgtcccaatgtgcagttccc
acgtctcaactgtgccacaatccttccatcccagtgcagagaccaacaatggcgtaccat
cattgctcaagattgtccatccgcctgtggcttctgtaatcaaggaggatgtgttgatgc
tgtcattgaatgtgcaaatgatatctcgatctgtaatgccgtcggaatgcaagacttcgt
aaatttgaactgccaaaagacttgcaacagatgctcaacaacaactgttggaggaatcat
tggaggttgcagcacctacaaccgtgactcaagcaatgcgtgtgccgcctgggctgtaaa
tggcttctgccaaaacaatttctacactcctgtcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGACGCACCAGTGCCTGGAGCT 698 73 207 Antisense
TACTGCATTGGCTGCTGCAACCGAT 116 645 258 Antisense
CGATCCGGTGGAACCAGACCTAGTG 426 247 279 Antisense
AGTGAAAACTGCAGCCGCTTCATGT 19 61 300 Antisense
CAAATGTCCCAATGTGCAGTTCCCA 351 193 363 Antisense
ATCCGCCTGTGGCTTCTGTAATCAA 248 39 465 Antisense
TATCTCGATCTGTAATGCCGTCGGA 539 657 528 Antisense
GGAGGTTGCAGCACCTACAACCGTG 597 515 628 Antisense
GACTCAAGCAATGCGTGTGCCGCCT 523 369 652 Antisense
TGTGCCGCCTGGGCTGTAAATGGCT 518 555 667 Antisense
AAAACAATTTCTACACTCCTGTCCA 6 131 698 Antisense
Sequence Source Affymetrix Proprietary Database