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[178789_at]

Description F25B3.2 /REP_DB=WormBase Gene ID /WP=CE05712 /TR=Q19769 /GB=CAA94754.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_high_8_8 > 06      6grp_highest_nonorm > 91      6grp_total > 77      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2692
1 2 3 4 range
A 70.7
(-3)
A 		111.8
(-2.9)
P 		144.7
(-2.8)
P 		2757.8
(3)
P 		2687
B 65.7
(-3)
M 		192.5
(-2.7)
P 		87.2
(-2.9)
P 		2485.2
(3)
P 		2420
C 77.4
(-3)
P 		170.6
(-2.8)
P 		107.9
(-2.9)
P 		2407.8
(3)
P 		2330
avr 71.3
(-3) 		158.3
(-2.8) 		113.3
(-2.9) 		2550.3
(3) 		2479

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 125.1
(-1.9)
P 		143.8
(-1.5)
P 		79.5
(-3)
P 		110.4
(-2.3)
P 		143.9
(-1.5)
P 		333.8
(3)
P 		254
B 69.2
(-3)
P 		105.4
(-2.1)
P 		105.4
(-2.1)
P 		152.9
(-0.8)
P 		178.3
(-0.2)
P 		300.5
(3)
P 		231
C 98.6
(-2.6)
P 		127.2
(-1.8)
P 		84.6
(-3)
P 		113.4
(-2.2)
P 		140.4
(-1.5)
P 		303.6
(3)
P 		219
avr 97.6
(-2.8) 		125.5
(-2) 		89.8
(-3) 		125.6
(-2) 		154.2
(-1.3) 		312.6
(3) 		223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 509
1 2 3 4 range
A 25.7
(-3)
A 		24.3
(-3)
P 		37.8
(-2.8)
P 		403.7
(3)
P 		379
B 21
(-3)
M 		47.6
(-2.7)
P 		20.2
(-3)
P 		529.6
(3)
P 		509
C 25.6
(-3)
P 		49
(-2.6)
P 		37.6
(-2.8)
P 		406
(3)
P 		380
avr 24.1
(-3) 		40.3
(-2.8) 		31.9
(-2.9) 		446.4
(3) 		422

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 23.1
(-2.5)
P 		51.1
(0.2)
P 		17.3
(-3)
P 		28
(-2)
P 		43.4
(-0.6)
P 		81.3
(3)
P 		64
B 24.1
(-3)
P 		35.1
(-2.2)
P 		25.6
(-2.9)
P 		40.5
(-1.9)
P 		72.4
(0.3)
P 		112
(3)
P 		88
C 36.5
(-1.9)
P 		38.8
(-1.7)
P 		21.5
(-3)
P 		22.8
(-2.9)
P 		45.4
(-1.2)
P 		101.6
(3)
P 		80
avr 27.9
(-2.5) 		41.7
(-1.4) 		21.5
(-3) 		30.4
(-2.3) 		53.7
(-0.5) 		98.3
(3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178789_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12456
Sequence Type Exemplar sequence
Representative Public ID F25B3.2 NCBI
Target Description F25B3.2 /REP_DB=WormBase Gene ID /WP=CE05712 /TR=Q19769 /GB=CAA94754.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073071(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073071 NCBI Caenorhabditis elegans F25B3.2 (F25B3.2) mRNA, complete cds. 11/11 None
SNAP00000011936 ENSEMBL cdna:SNAP chromosome:CEL140:V:9579937:9582530:1 10/11 None
GENEFINDER00000011940 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9579937:9582530:1 11/11 None
F25B3.2 ENSEMBL cdna:known chromosome:CEL140:V:9579712:9582530:1 gene:F25B3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9579711-9582530(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25B3.2
Entrez Gene 184914 Entrez gene
SwissProt Q19769 EMBL-EBI
WormBase CE05712 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94754 Hypothetical protein F25B3.2 [Caenorhabditis elegans] ref|NP_505472.1| F25B3.2 [Caenorhabditis elegans] 0.0
blast CAA94754 Hypothetical protein F25B3.2 [Caenorhabditis elegans] ref|NP_505472.1| F25B3.2 [Caenorhabditis elegans] 1.0E-170
blast CAA94754 Hypothetical protein F25B3.2 [Caenorhabditis elegans] ref|NP_505472.1| F25B3.2 [Caenorhabditis elegans] 1.0E-163
blast CAE72203 Hypothetical protein CBG19312 [Caenorhabditis briggsae] 1.0E-134
blast CAE72203 Hypothetical protein CBG19312 [Caenorhabditis briggsae] 1.0E-131
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505472.1 2 15-37,67-89
F25B3.2 2 15-37,67-89
Sequence
Target Sequence 
>C. ELEGANS:178789_AT
actttgccgagtcttacacaccaaatccacttgggaatacacttggagatgcggtatttg
ttgcacattttcagcagaccaatccgtgtgattcaagatgtcaaatgataaaagcagcgt
tgaatggtgaaatatctcgtcgttcatcttctccatcaaataatacaacaaatccattcg
tccgtgtttttggacgccttcgtgcatctgtattcagtgatgctcatcctactgtacctg
gtaattgtgcactcggaaacttcatccctgtcggaacatgttctgaccttggagaaacta
ttgatggaggcacacacgaatcgttgtgctctgaatgcagagggctgtacatgctcggtg
ctaattgttttccgaagattttcaacacagttcaatgtaattcacaggaaatgggatgta
tttttgacacgttcactgataaagctcatggtcaatgtggcattcagaccctctcattca
aagttctacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTGCCGAGTCTTACACACCAAA 47 101 635 Antisense
GCACATTTTCAGCAGACCAATCCGT 239 315 697 Antisense
TGAAATATCTCGTCGTTCATCTTCT 123 573 762 Antisense
CAAATCCATTCGTCCGTGTTTTTGG 288 191 803 Antisense
GCCTTCGTGCATCTGTATTCAGTGA 673 281 830 Antisense
CAGTGATGCTCATCCTACTGTACCT 462 207 849 Antisense
CTGTACCTGGTAATTGTGCACTCGG 495 225 866 Antisense
TTGTGCACTCGGAAACTTCATCCCT 288 709 879 Antisense
ACTTCATCCCTGTCGGAACATGTTC 298 103 893 Antisense
GCAGAGGGCTGTACATGCTCGGTGC 174 313 971 Antisense
GACCCTCTCATTCAAAGTTCTACGA 204 381 1101 Antisense
Sequence Source Affymetrix Proprietary Database