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[178768_s_at]

Description F25D7.2 /REP_DB=WormBase Gene ID /WP=CE09630 /TR=Q93559 /GB=CAB01694.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 49      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 75      6grp_total > 39      4grp_total > 16      6grp_cgc > 89      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 527
1 2 3 4 range
A 910.3
(-3)
P 		1017.7
(-1.7)
P 		1132.6
(-0.3)
P 		1409.7
(3)
P 		499
B 883.1
(-3)
P 		942.8
(-2.2)
P 		1096.5
(-0.3)
P 		1357
(3)
P 		474
C 889.1
(-3)
P 		941.5
(-2.4)
P 		987.7
(-1.8)
P 		1395.7
(3)
P 		507
avr 894.2
(-3) 		967.3
(-2.1) 		1072.3
(-0.8) 		1387.5
(3) 		493

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 566
1 2 3 4 5 6 range
A 1140.7
(1.7)
P 		957.5
(-0.2)
P 		698.8
(-3)
P 		748.8
(-2.5)
P 		1260.6
(3)
P 		1141
(1.7)
P 		562
B 936.9
(1.4)
P 		798.1
(-1.1)
P 		695
(-3)
P 		933.1
(1.4)
P 		986.8
(2.4)
P 		1022.1
(3)
P 		327
C 958.4
(0.5)
P 		697.9
(-3)
P 		933.2
(0.1)
P 		886
(-0.5)
P 		1150.9
(3)
P 		744.5
(-2.4)
P 		453
avr 1012
(1) 		817.8
(-2.3) 		775.7
(-3) 		856
(-1.7) 		1132.8
(3) 		969.2
(0.3) 		357
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 331.4
(3)
P 		220.9
(-2.3)
P 		296.1
(1.3)
P 		206.4
(-3)
P 		125
B 281.6
(2.2)
P 		232.9
(-3)
P 		254.1
(-0.7)
P 		289.2
(3)
P 		56
C 294.3
(0.3)
P 		270.7
(-1)
P 		344.2
(3)
P 		235.3
(-3)
P 		109
avr 302.4
(3) 		241.5
(-3) 		298.1
(2.6) 		243.6
(-2.8) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 210.3
(-1.5)
P 		340.5
(2)
P 		152.2
(-3)
P 		189.9
(-2)
P 		380.4
(3)
P 		277.8
(0.3)
P 		228
B 326
(1.1)
P 		265.6
(-0.5)
P 		168.8
(-3)
P 		247.2
(-1)
P 		400.9
(3)
P 		381.1
(2.5)
P 		232
C 355.2
(2.5)
P 		212.9
(-1.9)
P 		237.5
(-1.2)
P 		178.2
(-3)
P 		372.4
(3)
P 		249.2
(-0.8)
P 		194
avr 297.2
(0.4) 		273
(-0.4) 		186.2
(-3) 		205.1
(-2.4) 		384.6
(3) 		302.7
(0.5) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178768_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1609
Sequence Type Exemplar sequence
Representative Public ID F25D7.2 NCBI
Target Description F25D7.2 /REP_DB=WormBase Gene ID /WP=CE09630 /TR=Q93559 /GB=CAB01694.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060321(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060321 NCBI Caenorhabditis elegans F25D7.2 (F25D7.2) mRNA, complete cds. 11/11 None
SNAP00000011698 ENSEMBL cdna:SNAP chromosome:CEL140:I:10409193:10410581:1 11/11 None
GENEFINDER00000011703 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10409193:10410581:1 11/11 None
F25D7.2 ENSEMBL cdna:known chromosome:CEL140:I:10408569:10411062:1 gene:F25D7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10408499-10410512(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-353
Ensembl F25D7.2
Entrez Gene 172916 Entrez gene
SwissProt Q93559 EMBL-EBI
WormBase CE09630 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01694 Hypothetical protein F25D7.2 [Caenorhabditis elegans] ref|NP_492722.1| F25D7.2 [Caenorhabditis elegans] 0.0
blast CAB01694 Hypothetical protein F25D7.2 [Caenorhabditis elegans] ref|NP_492722.1| F25D7.2 [Caenorhabditis elegans] 1.0E-172
blast CAE58933 Hypothetical protein CBG02199 [Caenorhabditis briggsae] 1.0E-123
blast CAG10860 unnamed protein product [Tetraodon nigroviridis] 4.0E-13
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492722.1 2 273-295,366-383
F25D7.2 2 273-295,366-383
SNAP00000011698 2 241-263,334-351
GENEFINDER00000011703 2 241-263,334-351
Sequence
Target Sequence 
>C. ELEGANS:178768_S_AT
tcaagtttccgagcatcagcgattaatggctcactactacacatacgcatcgacaactcc
cgctgcaccaatttatcaagttcccgccaaccaacagcttcaacaaaatgcagtggcgtg
gagaattcgagttcaaggggtggccgctcccgctcgagccaacggaaatcaaggagctgc
agcgccaagacagggaagagttgacattttggaagtgggatatcgaattttcaaagtggt
tttgctattttctgcaattcttctctactcgtcattcgaaagattcgctttagttttatg
ctcggcactattcatttatttcattcaactgcgacgaaatcacgctcgcaatagagttca
agctcaggctgctgccgccgctgctcagcaagcaaataatcaagaaccacatgttaataa
caacaataatggagagaacgacggagataatacggctccaaccacggaaggagagacacc
agcaacttccgatgctccagtggaaccaccagctccacaaccaactggtatgcaagtgtt
cattgccactttatactcatttgtcacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAGTTTCCGAGCATCAGCGATTA 259 633 573 Antisense
TGGCTCACTACTACACATACGCATC 497 545 599 Antisense
CTGCACCAATTTATCAAGTTCCCGC 705 227 635 Antisense
ATTCGAGTTCAAGGGGTGGCCGCTC 221 9 697 Antisense
ATCAAGGAGCTGCAGCGCCAAGACA 307 27 740 Antisense
TTCGCTTTAGTTTTATGCTCGGCAC 222 697 856 Antisense
GCTCGGCACTATTCATTTATTTCAT 530 299 872 Antisense
TAATACGGCTCCAACCACGGAAGGA 544 625 1020 Antisense
AGAGACACCAGCAACTTCCGATGCT 232 69 1044 Antisense
CCGATGCTCCAGTGGAACCACCAGC 422 263 1061 Antisense
GCCACTTTATACTCATTTGTCACCT 119 279 1117 Antisense
Sequence Source Affymetrix Proprietary Database