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[178762_at]

Description F53A2.3 /REP_DB=WormBase Gene ID /WP=CE16095 /TR=O45548 /GB=CAB04451.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 31      6grp_total > 32      4grp_total > 61      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 143.3
(3)
P 		132.6
(1.9)
P 		121.3
(0.8)
P 		83.4
(-3)
A 		60
B 151.9
(3)
P 		111.1
(0.9)
P 		127.4
(1.8)
P 		33.8
(-3)
A 		118
C 166.5
(3)
P 		155.9
(2.1)
P 		143.1
(0.9)
P 		99.3
(-3)
A 		67
avr 153.9
(3) 		133.2
(1.5) 		130.6
(1.3) 		72.2
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 144.3
(1.4)
P 		152.3
(1.9)
P 		171.6
(3)
P 		129.8
(0.5)
P 		134.2
(0.8)
P 		69.3
(-3)
A 		102
B 167
(0.4)
P 		171
(0.7)
P 		200.5
(3)
P 		145.9
(-1.3)
P 		128
(-2.7)
P 		123.7
(-3)
P 		77
C 151
(1.4)
P 		173.1
(3)
P 		155.6
(1.8)
P 		112
(-1.4)
P 		89.1
(-3)
P 		117
(-1)
P 		84
avr 154.1
(1.2) 		165.5
(2.1) 		175.9
(3) 		129.2
(-0.9) 		117.1
(-1.9) 		103.3
(-3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 52.2
(3)
P 		28.8
(-0.5)
P 		31.7
(-0.1)
P 		12.2
(-3)
A 		40
B 48.4
(3)
P 		27.4
(-0.1)
P 		29.5
(0.2)
P 		7.2
(-3)
A 		41
C 55.1
(3)
P 		44.8
(1.4)
P 		49.9
(2.2)
P 		16.7
(-3)
A 		38
avr 51.9
(3) 		33.7
(0.3) 		37
(0.8) 		12
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 26.6
(-1.4)
P 		54.2
(3)
P 		37.4
(0.3)
P 		32.9
(-0.4)
P 		40.5
(0.8)
P 		16.9
(-3)
A 		37
B 58.1
(3)
P 		56.9
(2.6)
P 		48.7
(0.1)
P 		38.6
(-3)
P 		52
(1.1)
P 		46.1
(-0.7)
P 		20
C 56
(3)
P 		52.8
(2.4)
P 		39.6
(0.1)
P 		22.5
(-3)
P 		28.8
(-1.9)
P 		39.2
(-0)
P 		34
avr 46.9
(1) 		54.6
(3) 		41.9
(-0.3) 		31.3
(-3) 		40.4
(-0.7) 		34.1
(-2.3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178762_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6999
Sequence Type Exemplar sequence
Representative Public ID F53A2.3 NCBI
Target Description F53A2.3 /REP_DB=WormBase Gene ID /WP=CE16095 /TR=O45548 /GB=CAB04451.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067347(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067347 NCBI Caenorhabditis elegans F53A2.3 (F53A2.3) mRNA, complete cds. 11/11 None
SNAP00000020106 ENSEMBL cdna:SNAP chromosome:CEL140:III:13333897:13339276:-1 9/11 None
GENEFINDER00000020118 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13336566:13339276:-1 11/11 None
F53A2.3 ENSEMBL cdna:known chromosome:CEL140:III:13336566:13339276:-1 gene:F53A2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13336561-13339272(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53A2.3
Entrez Gene 186139 Entrez gene
SwissProt O45548 EMBL-EBI
WormBase CE16095 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04451 Hypothetical protein F53A2.3 [Caenorhabditis elegans] ref|NP_499748.1| F53A2.3 [Caenorhabditis elegans] 0.0
blast CAB04451 Hypothetical protein F53A2.3 [Caenorhabditis elegans] ref|NP_499748.1| F53A2.3 [Caenorhabditis elegans] 1.0E-164
blast AAG24173 Hypothetical protein R09E12.5 [Caenorhabditis elegans] ref|NP_503321.1| R09E12.5 [Caenorhabditis elegans] 7.0E-19
blast AAB71024 Hypothetical protein K02E7.2 [Caenorhabditis elegans] ref|NP_493908.1| K02E7.2 [Caenorhabditis elegans] 7.0E-19
Sequence
Target Sequence 
>C. ELEGANS:178762_AT
gaccgacttattcaacatggatcagaagacatgagaacagtgggttacggtatgagagga
atgatgaaattgttacaaattcaacatgaaaaattgatgggaaaaaagccggatgatgtg
acgactggcctgaaagccagtctcgttccagctcgttttttgaaagaaaaaacggttatc
gtcagaaatctgacagatgatgcggatattaatgtgaataaagtattcgggcagctaacg
gcacagtcatcttcactcaagaatatcgtgataaacgcggcaaacgtgtggatcaaaaat
gttgtcccggacgagcaacgaaaactctactcgaccagcataaaaccacaacaaccacaa
atcacttttccagctcaattattaaatggcccagcgggccttcttgtcagatgtctttct
gtgacaggaggagacaattcttcaaatgctgtgaacattagtctcctgttcaagcaacac
gtttctaatcttttcaatcacgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCGACTTATTCAACATGGATCAG 304 381 406 Antisense
TGGCCTGAAAGCCAGTCTCGTTCCA 333 545 531 Antisense
TCTCGTTCCAGCTCGTTTTTTGAAA 548 611 546 Antisense
TATTCGGGCAGCTAACGGCACAGTC 358 663 629 Antisense
GGCACAGTCATCTTCACTCAAGAAT 593 535 645 Antisense
GAAAACTCTACTCGACCAGCATAAA 229 355 725 Antisense
CAACCACAAATCACTTTTCCAGCTC 145 187 757 Antisense
TAAATGGCCCAGCGGGCCTTCTTGT 690 639 788 Antisense
GGCCTTCTTGTCAGATGTCTTTCTG 681 543 802 Antisense
GTCTCCTGTTCAAGCAACACGTTTC 134 467 866 Antisense
GTTTCTAATCTTTTCAATCACGCTC 263 451 886 Antisense
Sequence Source Affymetrix Proprietary Database