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[178757_at]

Description F19H8.4 /REP_DB=WormBase Gene ID /WP=CE23676 /TR=O45381 /GB=CAB07585.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 12      4grp_total > 49      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 258
1 2 3 4 range
A 10.2
(-3)
A 		266.7
(3)
P 		209.9
(1.7)
P 		21.8
(-2.7)
A 		257
B 12.6
(-3)
A 		171.6
(2)
P 		201.8
(3)
P 		11.5
(-3)
A 		190
C 9.2
(-3)
A 		207
(2.5)
P 		225.9
(3)
P 		10.2
(-3)
A 		217
avr 10.7
(-3) 		215.1
(3) 		212.5
(2.9) 		14.5
(-2.9) 		204

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 61.9
(-0.6)
A 		122.9
(3)
A 		72.5
(0)
A 		64.7
(-0.4)
A 		96.5
(1.4)
A 		21.3
(-3)
A 		102
B 67
(0.2)
A 		103.4
(3)
A 		82.9
(1.4)
A 		61
(-0.3)
A 		43.4
(-1.7)
A 		26.1
(-3)
A 		77
C 101.1
(1.4)
P 		134.7
(3)
A 		78.3
(0.3)
A 		66.7
(-0.3)
A 		15.6
(-2.7)
A 		9.9
(-3)
A 		125
avr 76.7
(0.4) 		120.3
(3) 		77.9
(0.5) 		64.1
(-0.3) 		51.8
(-1.1) 		19.1
(-3) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 3.7
(-2.9)
A 		57.9
(3)
P 		54.9
(2.7)
P 		3.2
(-3)
A 		55
B 4
(-2.8)
A 		42.4
(2.4)
P 		46.8
(3)
P 		2.4
(-3)
A 		44
C 3
(-2.9)
A 		59.5
(1.5)
P 		78.7
(3)
P 		1.7
(-3)
A 		77
avr 3.6
(-2.9) 		53.3
(2.3) 		60.1
(3) 		2.4
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 11.4
(-2)
A 		43.7
(3)
A 		15.8
(-1.3)
A 		16.4
(-1.3)
A 		29.1
(0.7)
A 		5.2
(-3)
A 		39
B 23.3
(0.3)
A 		34.4
(3)
A 		20.1
(-0.5)
A 		16.2
(-1.4)
A 		17.6
(-1.1)
A 		9.7
(-3)
A 		25
C 37.5
(2.4)
P 		41.1
(3)
A 		19.9
(-0.4)
A 		13.4
(-1.4)
A 		5
(-2.7)
A 		3.3
(-3)
A 		38
avr 24.1
(0.2) 		39.7
(3) 		18.6
(-0.8) 		15.3
(-1.4) 		17.2
(-1) 		6.1
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178757_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3904
Sequence Type Exemplar sequence
Representative Public ID F19H8.4 NCBI
Target Description F19H8.4 /REP_DB=WormBase Gene ID /WP=CE23676 /TR=O45381 /GB=CAB07585.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064637(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064637 NCBI Caenorhabditis elegans F19H8.4 (F19H8.4) mRNA, complete cds. 11/11 None
SNAP00000003961 ENSEMBL cdna:SNAP chromosome:CEL140:II:14620001:14622039:1 11/11 None
GENEFINDER00000003965 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14616597:14622039:1 11/11 None
F19H8.4 ENSEMBL cdna:known chromosome:CEL140:II:14616020:14622480:1 gene:F19H8.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14616375-14622036(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F19H8.4
Entrez Gene 175122 Entrez gene
SwissProt O45381 EMBL-EBI
WormBase CE32632 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07585 Hypothetical protein F19H8.4 [Caenorhabditis elegans] ref|NP_497038.2| F19H8.4 [Caenorhabditis elegans] 0.0
blast CAE65910 Hypothetical protein CBG11077 [Caenorhabditis briggsae] 0.0
blast CAB07585 Hypothetical protein F19H8.4 [Caenorhabditis elegans] ref|NP_497038.2| F19H8.4 [Caenorhabditis elegans] 1.0E-179
blast CAE65910 Hypothetical protein CBG11077 [Caenorhabditis briggsae] 1.0E-158
blast CAE17803 Hypothetical protein F19H8.5 [Caenorhabditis elegans] ref|NP_001022099.1| F19H8.5 [Caenorhabditis elegans] 5.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 6.8E-83
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:178757_AT
gcattgctttcggacttactggattgggatctccacaacgttcaaacttcactagcaata
gtcgtgtgaagcgtcagtcttcagtatggggaccgcatattctgactcctaccgttttgt
caccgattcttttcacaccaatttacgggctgaatgtgttgggtccaacagtcctctccc
ccggtctctttaccccactgatcctcaatccctcagtactctccccatacgtcttctcac
caacagtcggaatccccttcattttatccccgtatctcctgtccccatacgtgttctccc
cattagtcatggcgccgttcattcttaatccatatgttctctcacccaacgtcttcaatc
catacgttctgtcgccattgatcctgtcaccattggtcatctgccccgatgtggtgtctc
cgatgacgctgggtggagcgatcctgtcacctgcagtactgtcaccttcactgttgtcca
agtcgtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATTGCTTTCGGACTTACTGGATT 44 307 1781 Antisense
GATCTCCACAACGTTCAAACTTCAC 89 419 1808 Antisense
TATGGGGACCGCATATTCTGACTCC 326 655 1865 Antisense
TATTCTGACTCCTACCGTTTTGTCA 473 663 1878 Antisense
GTTTTGTCACCGATTCTTTTCACAC 667 447 1894 Antisense
ACGGGCTGAATGTGTTGGGTCCAAC 128 55 1925 Antisense
TTCTTAATCCATATGTTCTCTCACC 26 691 2102 Antisense
CACCCAACGTCTTCAATCCATACGT 304 201 2123 Antisense
GCCATTGATCCTGTCACCATTGGTC 99 281 2154 Antisense
TGACGCTGGGTGGAGCGATCCTGTC 601 569 2204 Antisense
ACCTTCACTGTTGTCCAAGTCGTAT 306 87 2244 Antisense
Sequence Source Affymetrix Proprietary Database