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[178753_at]

Description F28H6.4 /REP_DB=WormBase Gene ID /WP=CE23688 /TR=Q9U3H5 /GB=CAA20934.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 71      6grp_total > 30      4grp_total > 38      6grp_highest_sub > 02_03      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 275
1 2 3 4 range
A 454.8
(3)
P 		275.1
(-2.3)
P 		252.7
(-3)
P 		353.8
(0)
P 		202
B 506
(3)
P 		242.7
(-2.7)
P 		231.2
(-3)
P 		281.1
(-1.9)
P 		275
C 375.5
(3)
P 		341.8
(1.5)
P 		261.1
(-2.2)
P 		242.8
(-3)
P 		133
avr 445.4
(3) 		286.5
(-1.8) 		248.3
(-3) 		292.6
(-1.7) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 317
1 2 3 4 5 6 range
A 339
(-0.3)
P 		497.7
(3)
P 		373.7
(0.4)
P 		447.7
(2)
P 		207.4
(-3)
P 		284.9
(-1.4)
P 		290
B 313
(-1.7)
P 		411.6
(3)
P 		411.6
(3)
P 		392.2
(2.1)
P 		286
(-3)
P 		331.5
(-0.8)
P 		126
C 304.5
(-1.8)
P 		451
(1.4)
P 		524.3
(3)
P 		283.2
(-2.3)
P 		284.1
(-2.3)
P 		249.9
(-3)
P 		274
avr 318.8
(-1.2) 		453.4
(3) 		436.5
(2.5) 		374.4
(0.6) 		259.2
(-3) 		288.8
(-2.1) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 165.6
(3)
P 		59.7
(-2.6)
P 		66.1
(-2.2)
P 		51.8
(-3)
P 		114
B 161.4
(3)
P 		60
(-2.6)
P 		53.6
(-3)
P 		59.9
(-2.6)
P 		108
C 124.3
(3)
P 		98.3
(1.1)
P 		91
(0.6)
P 		40.9
(-3)
P 		83
avr 150.4
(3) 		72.7
(-1.7) 		70.2
(-1.8) 		50.9
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 62.5
(-3)
P 		177
(3)
P 		81.4
(-2)
P 		113.5
(-0.3)
P 		62.6
(-3)
P 		69.4
(-2.6)
P 		115
B 108.9
(-1.5)
P 		137
(3)
P 		99.9
(-3)
P 		103.9
(-2.4)
P 		116.2
(-0.4)
P 		123.6
(0.8)
P 		37
C 112.9
(1.2)
P 		137.6
(3)
P 		133.4
(2.7)
P 		57
(-3)
P 		91.9
(-0.4)
P 		83.7
(-1)
P 		81
avr 94.8
(-2.5) 		150.5
(3) 		104.9
(-1.5) 		91.5
(-2.9) 		90.2
(-3) 		92.2
(-2.8) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178753_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17989
Sequence Type Exemplar sequence
Representative Public ID F28H6.4 NCBI
Target Description F28H6.4 /REP_DB=WormBase Gene ID /WP=CE23688 /TR=Q9U3H5 /GB=CAA20934.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077953(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077953 NCBI Caenorhabditis elegans F28H6.4 (F28H6.4) mRNA, complete cds. 11/11 None
SNAP00000032399 ENSEMBL cdna:SNAP chromosome:CEL140:X:14127801:14133234:1 11/11 None
GENEFINDER00000032406 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14127801:14133234:1 11/11 None
F28H6.4 ENSEMBL cdna:known chromosome:CEL140:X:14127801:14133406:1 gene:F28H6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14127800-14133234(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28H6.4
Entrez Gene 181522 Entrez gene
SwissProt Q9U3H5 EMBL-EBI
WormBase CE23688 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA20934 Hypothetical protein F28H6.4 [Caenorhabditis elegans] ref|NP_510354.1| F28H6.4 [Caenorhabditis elegans] 0.0
blast C89701 protein F28H6.5 [imported] - Caenorhabditis elegans 1.0E-156
blast CAH19090 Hypothetical protein B0019.2 [Caenorhabditis elegans] emb|CAA15508.2| Hypothetical protein B0019.2 [Caenorhabditis elegans] ref|NP_493237.2| B0019.2 [Caenorhabditis elegans] 4.0E-80
Sequence
Target Sequence 
>C. ELEGANS:178753_AT
acagagccactgtcaacatctcgtacatctaagcctgtgccatatttttcaaatgccacg
atggcagcgatctaccaatcatttagcccacttccatgcgcaactccacctgttcagtta
tgctatcgtataactgccaacgaagttacccgtcgcaagcagaccgaggaggccgagttt
gaattggcgctcacaactgcgaaaacacttacactcgaactccaagccagaaatgaggga
gcatcgacttcatctccatcgggattacaaaacagtggccgatccgaggttgccagaaat
atggacaccgcatttacggaaattgaagcaagcattcgactgttcaattcccgtctgaag
gatgtgtcgcataatcttaatgacggacagcgtggagcttacgcagaccgagcaaatgct
ctatcgcggatgttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGAGCCACTGTCAACATCTCGTA 395 107 2527 Antisense
ACATCTCGTACATCTAAGCCTGTGC 98 105 2542 Antisense
TGCCACGATGGCAGCGATCTACCAA 441 585 2580 Antisense
GATCTACCAATCATTTAGCCCACTT 109 419 2595 Antisense
CAACTCCACCTGTTCAGTTATGCTA 663 187 2627 Antisense
TTACCCGTCGCAAGCAGACCGAGGA 68 683 2672 Antisense
GAGGCCGAGTTTGAATTGGCGCTCA 587 405 2695 Antisense
GAAAACACTTACACTCGAACTCCAA 381 355 2727 Antisense
AGGGAGCATCGACTTCATCTCCATC 246 59 2762 Antisense
AAACAGTGGCCGATCCGAGGTTGCC 679 123 2796 Antisense
GCAAATGCTCTATCGCGGATGTTCC 54 319 2938 Antisense
Sequence Source Affymetrix Proprietary Database