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[178752_at]

Description W06A7.4 /REP_DB=WormBase Gene ID /WP=CE14670 /TR=Q23189 /GB=CAB01524.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 43      6grp_highest_nonorm > 80      6grp_total > 10      4grp_total > 49      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 313
1 2 3 4 range
A 13.4
(-3)
A 		322.6
(3)
P 		314.5
(2.8)
P 		22.2
(-2.8)
A 		309
B 9.5
(-3)
A 		280.2
(3)
P 		271.8
(2.8)
P 		18.1
(-2.8)
A 		271
C 33.4
(-2.9)
A 		234.2
(1.3)
P 		311.8
(3)
P 		30.1
(-3)
A 		282
avr 18.8
(-3) 		279
(2.6) 		299.4
(3) 		23.5
(-2.9) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 285
1 2 3 4 5 6 range
A 109.7
(-3)
P 		363
(3)
P 		237.5
(0)
P 		220.9
(-0.4)
P 		267.5
(0.7)
P 		113.3
(-2.9)
P 		253
B 100
(-3)
P 		369.5
(3)
P 		352.3
(2.6)
P 		196.4
(-0.9)
P 		277.3
(0.9)
P 		149.6
(-1.9)
P 		270
C 84.6
(-3)
P 		281.8
(3)
P 		164.6
(-0.6)
P 		259.1
(2.3)
P 		225.9
(1.3)
P 		173.5
(-0.3)
P 		197
avr 98.1
(-3) 		338.1
(3) 		251.5
(0.8) 		225.5
(0.2) 		256.9
(1) 		145.5
(-1.8) 		240
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 4.9
(-2.9)
A 		70
(2.1)
P 		82.2
(3)
P 		3.3
(-3)
A 		79
B 3
(-3)
A 		69.2
(3)
P 		63
(2.4)
P 		3.9
(-2.9)
A 		66
C 11
(-2.7)
A 		67.3
(0.6)
P 		108.7
(3)
P 		5.1
(-3)
A 		104
avr 6.3
(-2.8) 		68.8
(1.8) 		84.6
(3) 		4.1
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 20.2
(-3)
P 		129.1
(3)
P 		51.7
(-1.3)
P 		56
(-1)
P 		80.7
(0.3)
P 		27.6
(-2.6)
P 		109
B 34.8
(-3)
P 		123
(3)
P 		85.5
(0.4)
P 		52
(-1.8)
P 		112.7
(2.3)
P 		55.8
(-1.6)
P 		88
C 31.4
(-3)
P 		86
(3)
P 		41.9
(-1.8)
P 		52.1
(-0.7)
P 		73.1
(1.6)
P 		58.1
(-0.1)
P 		55
avr 28.8
(-3) 		112.7
(3) 		59.7
(-0.8) 		53.4
(-1.2) 		88.8
(1.3) 		47.2
(-1.7) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178752_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14421
Sequence Type Exemplar sequence
Representative Public ID W06A7.4 NCBI
Target Description W06A7.4 /REP_DB=WormBase Gene ID /WP=CE14670 /TR=Q23189 /GB=CAB01524.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074254(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074254 NCBI Caenorhabditis elegans W06A7.4 (W06A7.4) mRNA, complete cds. 10/11 None
GENEFINDER00000021528 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14827253:14831784:1 10/11 None
W06A7.4 ENSEMBL cdna:known chromosome:CEL140:V:14829916:14831784:1 gene:W06A7.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021520 2/11 Cross Hyb Matching Probes None
SNAP00000021521 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14829915-14831784(+) 94.42 100.0
Public Domain and Genome References
Gene Title
Ensembl W06A7.4
Entrez Gene 189232 Entrez gene
SwissProt Q23189 EMBL-EBI
WormBase CE28967 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01524 Hypothetical protein W06A7.4 [Caenorhabditis elegans] ref|NP_506655.1| W06A7.4 [Caenorhabditis elegans] 1.0E-127
blast CAB01524 Hypothetical protein W06A7.4 [Caenorhabditis elegans] ref|NP_506655.1| W06A7.4 [Caenorhabditis elegans] 1.0E-120
blast CAE66365 Hypothetical protein CBG11622 [Caenorhabditis briggsae] 7.0E-90
blast CAE66365 Hypothetical protein CBG11622 [Caenorhabditis briggsae] 4.0E-89
blast CAC70102 Hypothetical protein T19A6.4 [Caenorhabditis elegans] ref|NP_492278.2| T19A6.4 [Caenorhabditis elegans] 2.0E-58
blast CAC70102 Hypothetical protein T19A6.4 [Caenorhabditis elegans] ref|NP_492278.2| T19A6.4 [Caenorhabditis elegans] 1.0E-47
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506655.1 7 7-29,44-66,98-120,140-162,174-196,201-223,232-254
W06A7.4 7 7-29,44-66,98-120,140-162,174-196,201-223,232-254
GENEFINDER00000021528 8 23-45,58-80,90-112,144-166,186-208,220-242,247-269,278-300
Sequence
Target Sequence 
>C. ELEGANS:178752_AT
atgttcatgctttttgcgtcttcaatatcttgtctgacaggctgggcaatatctagattc
ggtctatttggtgtaccttccgccgttccaatgagcattttactcaattataatgggatt
attttactaatagttggatcaaaccgtagtgggacagcccggcaccacgtgagttttcag
acccgtcacgacatctacccaaaatcatctgaaattacgtcacccgtctcgtttcacttt
tcatttttcacaggagtaatgattgtttcagtaataatcttcattgcttattgttttgtg
cgcaagggaaaaccctcaattcctccaaaaatagtgattccttcaatgctcagtggtgta
ttcttttctggagcaatggcttgtttcttcattgccaatgaacagctatcaccaacaatt
tcttatccaatctgtatgatggcacctggatggataacttcagcatggagcgttttctat
ttcagagaaatttctggtcgccgcaatctacttctcctcggtactgcatacggcttcaca
cttttcggagttcttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTCATGCTTTTTGCGTCTTCAA 470 47 34 Antisense
TCGGTCTATTTGGTGTACCTTCCGC 574 603 92 Antisense
CACCACGTGAGTTTTCAGACCCGTC 307 199 196 Antisense
GTCACGACATCTACCCAAAATCATC 405 465 218 Antisense
CATCTGAAATTACGTCACCCGTCTC 591 211 239 Antisense
GATTCCTTCAATGCTCAGTGGTGTA 521 433 369 Antisense
AGTGGTGTATTCTTTTCTGGAGCAA 39 59 385 Antisense
CTGGAGCAATGGCTTGTTTCTTCAT 426 241 401 Antisense
AACAGCTATCACCAACAATTTCTTA 325 137 434 Antisense
CAGCATGGAGCGTTTTCTATTTCAG 619 203 494 Antisense
GCTTCACACTTTTCGGAGTTCTTGT 270 305 566 Antisense
Sequence Source Affymetrix Proprietary Database