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[178750_at]

Description F21G4.5 /REP_DB=WormBase Gene ID /WP=CE09551 /TR=Q93549 /GB=CAB02664.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 17      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 34.4
(-3)
A 		53.4
(-0.4)
P 		39.8
(-2.3)
A 		78.9
(3)
P 		45
B 34.2
(-3)
A 		37.6
(-1.1)
A 		45.1
(3)
A 		37.2
(-1.3)
A 		11
C 26.6
(-3)
A 		49.5
(0)
A 		36.5
(-1.7)
M 		72.4
(3)
P 		46
avr 31.7
(-3) 		46.8
(-0.1) 		40.5
(-1.3) 		62.8
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 6
(-3)
A 		39.6
(0.5)
A 		51.4
(1.7)
A 		63.5
(3)
P 		25.2
(-1)
A 		20.7
(-1.5)
A 		58
B 40.8
(2.7)
P 		42.4
(3)
A 		41.2
(2.8)
A 		5.3
(-3)
A 		38.5
(2.4)
A 		21.8
(-0.3)
A 		37
C 26.3
(-1.4)
P 		55.2
(3)
A 		24.6
(-1.7)
A 		44.5
(1.4)
A 		16.1
(-3)
A 		32.8
(-0.4)
A 		39
avr 24.4
(-3) 		45.7
(3) 		39.1
(1.1) 		37.8
(0.8) 		26.6
(-2.4) 		25.1
(-2.8) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 12.5
(3)
A 		11.6
(0.4)
P 		10.4
(-3)
A 		11.6
(0.4)
P 		2
B 10.9
(3)
A 		9.3
(-0.2)
A 		10.4
(2)
A 		7.9
(-3)
A 		3
C 8.8
(-3)
A 		14.2
(3)
A 		12.7
(1.3)
M 		12.2
(0.8)
P 		5
avr 10.7
(-2.5) 		11.7
(3) 		11.2
(0.3) 		10.6
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 1.1
(-3)
A 		14.1
(2.2)
A 		11.2
(1)
A 		16.1
(3)
P 		7.6
(-0.4)
A 		5
(-1.4)
A 		15
B 14.2
(2.4)
P 		14.1
(2.4)
A 		10
(0.6)
A 		1.4
(-3)
A 		15.6
(3)
A 		8.1
(-0.2)
A 		14
C 9.7
(-0.7)
P 		16.9
(3)
A 		6.3
(-2.4)
A 		9
(-1.1)
A 		5.2
(-3)
A 		11
(-0)
A 		12
avr 8.3
(-2.7) 		15
(3) 		9.2
(-2) 		8.8
(-2.3) 		9.5
(-1.7) 		8
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178750_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17267
Sequence Type Exemplar sequence
Representative Public ID F21G4.5 NCBI
Target Description F21G4.5 /REP_DB=WormBase Gene ID /WP=CE09551 /TR=Q93549 /GB=CAB02664.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077261(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077261 NCBI Caenorhabditis elegans F21G4.5 (F21G4.5) mRNA, complete cds. 11/11 None
SNAP00000036633 ENSEMBL cdna:SNAP chromosome:CEL140:X:9906086:9907735:1 11/11 None
GENEFINDER00000036640 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9906086:9907735:1 11/11 None
F21G4.5 ENSEMBL cdna:known chromosome:CEL140:X:9906086:9907735:1 gene:F21G4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9906085-9907735(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21G4.5
Entrez Gene 184794 Entrez gene
SwissProt Q93549 EMBL-EBI
WormBase CE09551 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02664 Hypothetical protein F21G4.5 [Caenorhabditis elegans] ref|NP_509662.1| F21G4.5 [Caenorhabditis elegans] 0.0
blast CAA94210 Hypothetical protein K04C1.3 [Caenorhabditis elegans] ref|NP_510374.1| K04C1.3 [Caenorhabditis elegans] 3.0E-35
blast CAE58548 Hypothetical protein CBG01707 [Caenorhabditis briggsae] 7.0E-30
Sequence
Target Sequence 
>C. ELEGANS:178750_AT
agtaatttcaccattcgttgttccttggattctccacttgcctcgtttttgtcaatgcaa
tatttaaaaatatatctggatgaggtgccgtcaaataaatcatttactcggaaatatgtg
aaagttgaattgcagcgcccagaaaaacaatacaatattcatttcgacttcacgtttatt
gatagagcggttacaagaataaaaaccgagcgtgagacgaagaaacaaaaaaaattagtg
gaatactacgagaaaaaatgtcaacgagcacaggttgccgaagaagacctgctttttaaa
aagaacagaaccgaagaagaaacaaagtgcttggcacttgcattgaatttaccggaaaat
ttcacgcatatgcagctatatatggctatgaaatcaatggccgatcataagccaaacctt
ccagaaaacactctgaaatatgatgctgcaattgatcaggagggaaattatatgacaaaa
gagcagcgaaaattgatttcaacgctgtcaaacaacttcatgtacgtttcgggaagcaat
cgtcgttgcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTAATTTCACCATTCGTTGTTCCT 141 61 412 Antisense
CATTCGTTGTTCCTTGGATTCTCCA 366 217 423 Antisense
CACTTGCCTCGTTTTTGTCAATGCA 314 197 446 Antisense
GTGAAAGTTGAATTGCAGCGCCCAG 519 481 529 Antisense
ATATTCATTTCGACTTCACGTTTAT 415 19 566 Antisense
ATGTCAACGAGCACAGGTTGCCGAA 225 47 669 Antisense
GTTGCCGAAGAAGACCTGCTTTTTA 16 437 685 Antisense
TGGCACTTGCATTGAATTTACCGGA 639 545 743 Antisense
GGCCGATCATAAGCCAAACCTTCCA 642 541 810 Antisense
TCAACGCTGTCAAACAACTTCATGT 591 629 910 Antisense
TTCGGGAAGCAATCGTCGTTGCACC 392 693 939 Antisense
Sequence Source Affymetrix Proprietary Database