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[178745_at]

Description T25C8.1 /REP_DB=WormBase Gene ID /WP=CE16462 /TR=O45814 /GB=CAB05816.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 14      4grp_total > 11      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 87.9
(-1)
P 		70.4
(-2.7)
A 		66.7
(-3)
A 		131.5
(3)
P 		65
B 52.8
(-3)
P 		58.4
(-2.7)
A 		61.5
(-2.6)
A 		178.6
(3)
P 		126
C 74.5
(-0.8)
P 		79.8
(-0.5)
A 		36.2
(-3)
A 		142.2
(3)
M 		106
avr 71.7
(-1.9) 		69.5
(-2.1) 		54.8
(-3) 		150.8
(3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 106.3
(3)
P 		84.4
(1.6)
P 		49.4
(-0.7)
A 		77.5
(1.1)
A 		14.1
(-3)
A 		60.8
(0)
A 		92
B 72.6
(0.6)
M 		71.5
(0.5)
P 		92.1
(3)
P 		48.3
(-2.3)
M 		56.1
(-1.3)
A 		42.4
(-3)
A 		50
C 78.1
(1.8)
A 		68.4
(0.8)
A 		29.5
(-3)
A 		90.4
(3)
A 		54.6
(-0.5)
P 		50.7
(-0.9)
A 		61
avr 85.7
(3) 		74.8
(1.5) 		57
(-0.9) 		72.1
(1.1) 		41.6
(-3) 		51.3
(-1.7) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 32
(3)
P 		15.3
(-3)
A 		17.4
(-2.2)
A 		19.3
(-1.6)
P 		17
B 16.8
(-2.4)
P 		14.4
(-3)
A 		14.3
(-3)
A 		38.1
(3)
P 		24
C 24.7
(3)
P 		22.9
(2.1)
A 		12.6
(-3)
A 		24
(2.7)
M 		12
avr 24.5
(1.7) 		17.5
(-1.7) 		14.8
(-3) 		27.1
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 19.6
(0.6)
P 		30
(3)
P 		10.7
(-1.5)
A 		19.7
(0.6)
A 		4.3
(-3)
A 		14.8
(-0.5)
A 		26
B 25.3
(3)
M 		23.8
(2.3)
P 		22.4
(1.6)
P 		12.8
(-3)
M 		22.8
(1.8)
A 		15.8
(-1.6)
A 		13
C 28.9
(3)
A 		20.9
(0.8)
A 		7.5
(-3)
A 		18.2
(0)
A 		17.7
(-0.1)
P 		17
(-0.3)
A 		21
avr 24.6
(2.8) 		24.9
(3) 		13.5
(-3) 		16.9
(-1.2) 		14.9
(-2.3) 		15.9
(-1.7) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178745_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7796
Sequence Type Exemplar sequence
Representative Public ID T25C8.1 NCBI
Target Description T25C8.1 /REP_DB=WormBase Gene ID /WP=CE16462 /TR=O45814 /GB=CAB05816.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067409(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067409 NCBI Caenorhabditis elegans T25C8.1 (T25C8.1) mRNA, complete cds. 10/11 None
GENEFINDER00000008512 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13607588:13609264:1 11/11 None
T25C8.1 ENSEMBL cdna:known chromosome:CEL140:III:13607588:13609264:1 gene:T25C8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000008507 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13607583-13609260(+) 97.51 100.0
Public Domain and Genome References
Gene Title
Ensembl T25C8.1
Entrez Gene 188886 Entrez gene
SwissProt O45814 EMBL-EBI
WormBase CE38424 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.29551.1.S1_AT similar to Carbohydrate kinase-like protein cfa
CHICKEN:GGA.11708.1.S1_AT similar to Carbohydrate kinase-like protein gga
HG-U133A:219712_S_AT carbohydrate kinase-like hs
HG-U133_PLUS_2:219712_S_AT carbohydrate kinase-like hs
HG-U133A_2:219712_S_AT carbohydrate kinase-like hs
HG-FOCUS:219712_S_AT carbohydrate kinase-like hs
HG-U133A:219713_AT carbohydrate kinase-like hs
HG-U133_PLUS_2:219713_AT carbohydrate kinase-like hs
HG-U133A_2:219713_AT carbohydrate kinase-like hs
U133_X3P:G7019340_3P_S_AT carbohydrate kinase-like hs
U133_X3P:G7019340_3P_AT carbohydrate kinase-like hs
HG-U95E:85653_AT carbohydrate kinase-like hs
U133_X3P:HS2.268606.1.A1_3P_AT Carbohydrate kinase-like hs
HG-U133_PLUS_2:1556229_AT Carbohydrate kinase-like hs
HG-U95E:86079_R_AT Carbohydrate kinase-like hs
HG-U133_PLUS_2:236867_AT Carbohydrate kinase-like hs
HG-U133B:236867_AT Carbohydrate kinase-like hs
HG-U95B:59340_AT Carbohydrate kinase-like hs
HU35KSUBA:RC_AA010211_AT Carbohydrate kinase-like hs
U133_X3P:HS.16241.0.A1_3P_AT Carbohydrate kinase-like hs
MG-U74BV2:106264_AT carbohydrate kinase-like mm
MOE430B:1452853_AT carbohydrate kinase-like mm
MOUSE430_2:1452853_AT carbohydrate kinase-like mm
MU19KSUBB:TC31148_AT Carbohydrate kinase-like (Carkl), mRNA mm
RAE230B:1384273_AT Carbohydrate kinase-like (predicted) rn
RAT230_2:1384273_AT Carbohydrate kinase-like (predicted) rn
RG-U34B:RC_AI070132_AT Carbohydrate kinase-like (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB05816 Hypothetical protein T25C8.1 [Caenorhabditis elegans] ref|NP_499810.2| T25C8.1 [Caenorhabditis elegans] 0.0
blast CAB05816 Hypothetical protein T25C8.1 [Caenorhabditis elegans] ref|NP_499810.2| T25C8.1 [Caenorhabditis elegans] 1.0E-178
blast CAB05816 Hypothetical protein T25C8.1 [Caenorhabditis elegans] ref|NP_499810.2| T25C8.1 [Caenorhabditis elegans] 1.0E-173
blast CAE71352 Hypothetical protein CBG18255 [Caenorhabditis briggsae] 1.0E-138
blast CAE71352 Hypothetical protein CBG18255 [Caenorhabditis briggsae] 1.0E-102
blast CAE71352 Hypothetical protein CBG18255 [Caenorhabditis briggsae] 1.0E-100
blast AAI01915 Carbohydrate kinase-like [Rattus norvegicus] ref|NP_001028854.1| carbohydrate kinase-like [Rattus norvegicus] 9.0E-27
blast AAI01915 Carbohydrate kinase-like [Rattus norvegicus] ref|NP_001028854.1| carbohydrate kinase-like [Rattus norvegicus] 7.0E-24
blast AAI01915 Carbohydrate kinase-like [Rattus norvegicus] ref|NP_001028854.1| carbohydrate kinase-like [Rattus norvegicus] 1.0E-22
Sequence
Target Sequence 
>C. ELEGANS:178745_AT
ggacatacagttctacggagctcccagcgtttctaaaaactattcctaaatgggaatgtg
gagatttgcatccagggtttggtcttgttacgctggcgtttttgaactacagtaatccgg
aatttttaacgaattttatacattgtggaaccataatggacctattcatctctattttga
cacaaaacccggaccccctcatctctcaccacaacgctcacagctggggttactgtactg
aaaacgctacgtggcagtcagaaattttagattttctccctaaaactctgaaactacctg
acataaattcttcctctgaagtttcggggacttggcacggtgccaagtgccacgtggcat
caggagatttgcaagcttccgtggcatcgctggattattatgaaaataccgcttatgtaa
tcctcggcacatctgctcaactatgttgccttattcaaacttcggaaaaaaatgctacag
taatcccggctaccgtagtccggctgccattctccacgtcgaaaactctggtggccgcct
gcgcaatgaacgggggaaatgctctggaagcgacgttgaaggtgagcctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACATACAGTTCTACGGAGCTCCC 590 525 347 Antisense
CAGGGTTTGGTCTTGTTACGCTGGC 26 207 419 Antisense
AATGGACCTATTCATCTCTATTTTG 678 161 501 Antisense
CACAACGCTCACAGCTGGGGTTACT 420 193 556 Antisense
CTACCTGACATAAATTCTTCCTCTG 425 225 640 Antisense
CACGGTGCCAAGTGCCACGTGGCAT 136 199 682 Antisense
GAGATTTGCAAGCTTCCGTGGCATC 408 397 710 Antisense
ATACCGCTTATGTAATCCTCGGCAC 475 23 752 Antisense
GGCACATCTGCTCAACTATGTTGCC 641 535 772 Antisense
GCCGCCTGCGCAATGAACGGGGGAA 388 285 880 Antisense
GAAGCGACGTTGAAGGTGAGCCTGG 70 343 913 Antisense
Sequence Source Affymetrix Proprietary Database