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[178739_at]

Description F35E8.9 /REP_DB=WormBase Gene ID /WP=CE15959 /TR=O45450 /GB=CAB04292.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 51      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 62.8
(-0.2)
A 		118.6
(3)
A 		79.8
(0.8)
A 		14.2
(-3)
A 		104
B 14.9
(-3)
A 		63.8
(0.9)
A 		90.7
(3)
A 		15.9
(-2.9)
A 		76
C 92.4
(-0.5)
A 		193.9
(3)
A 		35.1
(-2.4)
A 		18.8
(-3)
A 		175
avr 56.7
(-0.8) 		125.4
(3) 		68.5
(-0.1) 		16.3
(-3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 12.5
(-2.9)
A 		65
(2.5)
A 		61.2
(2.2)
A 		11.2
(-3)
A 		69.4
(3)
A 		59.9
(2)
A 		58
B 31.9
(-1.2)
A 		74.4
(3)
A 		13.5
(-3)
A 		13.5
(-3)
A 		49.4
(0.5)
A 		29
(-1.5)
A 		61
C 76.4
(3)
A 		54.4
(0.9)
A 		22
(-2.2)
A 		13.7
(-3)
A 		57.7
(1.2)
A 		31.1
(-1.3)
A 		63
avr 40.3
(0.2) 		64.6
(3) 		32.2
(-0.8) 		12.8
(-3) 		58.8
(2.3) 		40
(0.2) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 22.8
(2.3)
A 		25.7
(3)
A 		20.9
(1.8)
A 		2.1
(-3)
A 		24
B 4.8
(-2.5)
A 		15.8
(1.2)
A 		21
(3)
A 		3.4
(-3)
A 		18
C 30.6
(0.1)
A 		55.7
(3)
A 		12.2
(-2)
A 		3.2
(-3)
A 		53
avr 19.4
(0.4) 		32.4
(3) 		18
(0.1) 		2.9
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 2.3
(-3)
A 		23.1
(3)
A 		13.3
(0.2)
A 		2.8
(-2.9)
A 		21
(2.4)
A 		14.6
(0.5)
A 		21
B 11.1
(-0.8)
A 		24.8
(3)
A 		3.3
(-3)
A 		3.6
(-2.9)
A 		20.1
(1.7)
A 		10.8
(-0.9)
A 		22
C 28.3
(3)
A 		16.6
(0.2)
A 		5.6
(-2.3)
A 		2.8
(-3)
A 		18.7
(0.7)
A 		10.4
(-1.2)
A 		26
avr 13.9
(0.5) 		21.5
(3) 		7.4
(-1.6) 		3.1
(-3) 		19.9
(2.5) 		11.9
(-0.1) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178739_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13874
Sequence Type Exemplar sequence
Representative Public ID F35E8.9 NCBI
Target Description F35E8.9 /REP_DB=WormBase Gene ID /WP=CE15959 /TR=O45450 /GB=CAB04292.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074583(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074583 NCBI Caenorhabditis elegans F35E8.9 (F35E8.9) mRNA, complete cds. 11/11 None
SNAP00000019515 ENSEMBL cdna:SNAP chromosome:CEL140:V:15918918:15920142:-1 11/11 None
GENEFINDER00000019527 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15918918:15920142:-1 11/11 None
F35E8.9 ENSEMBL cdna:known chromosome:CEL140:V:15918918:15920142:-1 gene:F35E8.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15918917-15920142(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E8.9
Entrez Gene 185300 Entrez gene
SwissProt O45450 EMBL-EBI
WormBase CE15959 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04292 Hypothetical protein F35E8.9 [Caenorhabditis elegans] ref|NP_506984.1| F35E8.9 [Caenorhabditis elegans] 1.0E-134
blast CAB03300 Hypothetical protein T06C12.14 [Caenorhabditis elegans] ref|NP_506976.1| T06C12.14 [Caenorhabditis elegans] 1.0E-118
blast CAB04297 Hypothetical protein F35E8.13 [Caenorhabditis elegans] emb|CAB04291.1| Hypothetical protein F35E8.10 [Caenorhabditis elegans] ref|NP_506988.1| F35E8.13 [Caenorhabditis elegans] ref|NP_506985.1| F35E8.10 [Caenorhabditis elegans] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.2E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.3E-11
Sequence
Target Sequence 
>C. ELEGANS:178739_AT
cccaaacccagttccagctgatggatacccatcaccaggcagggaccagcagagaccatt
ggcttgttatactactgctacggctactccagctgctgtagttcaagacatgaagactgc
agctcagagcacatgcccaagaacttgcggattatgttgtcaaactagtgcttacagttg
cccaaatgttgcctacccccgtctcaactgtgcctcgatcaccgcctctcaatgcatctc
cccagcctggagaacaatcatagccacggactgcccatctgcctgcggattctgcaacca
aggaggatgtgtggatgccgttcctgactgctccaatgacatcaccatttgcaatgccat
cggccttcagactttcgtgaacgccaactgtcagagaacttgcaatagatgtgcctcgtc
aacaactactctttcttcatcttccttgacctgctcctcatacatcgccgactcgagcac
tgcatgcagagcctggtctctgaacggattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCAAACCCAGTTCCAGCTGATGGA 139 257 180 Antisense
TGGATACCCATCACCAGGCAGGGAC 538 549 201 Antisense
CAGGCAGGGACCAGCAGAGACCATT 371 209 215 Antisense
GTTATACTACTGCTACGGCTACTCC 561 447 245 Antisense
TACAGTTGCCCAAATGTTGCCTACC 303 643 352 Antisense
AGAACAATCATAGCCACGGACTGCC 540 71 430 Antisense
GAGGATGTGTGGATGCCGTTCCTGA 633 403 482 Antisense
TTTGCAATGCCATCGGCCTTCAGAC 584 667 527 Antisense
GACTTTCGTGAACGCCAACTGTCAG 234 373 549 Antisense
ATAGATGTGCCTCGTCAACAACTAC 38 21 584 Antisense
AGAGCCTGGTCTCTGAACGGATTCT 459 69 667 Antisense
Sequence Source Affymetrix Proprietary Database