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[178735_at]

Description C27D8.2 /REP_DB=WormBase Gene ID /WP=CE08421 /TR=Q93289 /GB=CAB02262.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 63      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 27.8
(-0.1)
A 		18
(-3)
A 		38.1
(3)
A 		31.7
(1.1)
A 		20
B 55.1
(2.7)
A 		22.9
(-1.2)
A 		57.2
(3)
A 		8
(-3)
A 		49
C 46.5
(0.6)
A 		27.3
(-3)
A 		31.6
(-2.2)
A 		59.4
(3)
A 		32
avr 43.1
(3) 		22.7
(-3) 		42.3
(2.8) 		33
(0) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 64.1
(-1.8)
A 		49.3
(-2.2)
A 		25.2
(-3)
A 		50.3
(-2.2)
P 		88.1
(-1)
P 		216.6
(3)
P 		191
B 10.5
(-2.8)
A 		28.6
(-1.9)
A 		6.5
(-3)
A 		48.2
(-0.9)
A 		100.9
(1.7)
P 		125.9
(3)
P 		119
C 74.6
(-1.3)
A 		48.2
(-2.3)
A 		29.5
(-3)
A 		63.5
(-1.7)
A 		91.1
(-0.7)
P 		187.3
(3)
P 		158
avr 49.7
(-1.9) 		42
(-2.2) 		20.4
(-3) 		54
(-1.7) 		93.4
(-0.2) 		176.6
(3) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 10.1
(3)
A 		3.9
(-3)
A 		10
(2.9)
A 		4.6
(-2.3)
A 		6
B 17.6
(3)
A 		5.7
(-1.5)
A 		13.3
(1.4)
A 		1.7
(-3)
A 		16
C 15.4
(3)
A 		7.8
(-3)
A 		11
(-0.5)
A 		10
(-1.3)
A 		8
avr 14.4
(3) 		5.8
(-2.7) 		11.4
(1) 		5.4
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 11.8
(-2.2)
A 		17.5
(-1.5)
A 		5.5
(-3)
A 		12.8
(-2.1)
P 		26.6
(-0.3)
P 		52.7
(3)
P 		47
B 3.7
(-2.7)
A 		9.5
(-2)
A 		1.6
(-3)
A 		12.8
(-1.5)
A 		41
(2.2)
P 		46.9
(3)
P 		45
C 27.7
(-0.8)
A 		14.7
(-2.2)
A 		7.5
(-3)
A 		12.8
(-2.4)
A 		29.5
(-0.6)
P 		62.7
(3)
P 		55
avr 14.4
(-1.8) 		13.9
(-1.9) 		4.9
(-3) 		12.8
(-2) 		32.4
(0.4) 		54.1
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178735_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8737
Sequence Type Exemplar sequence
Representative Public ID C27D8.2 NCBI
Target Description C27D8.2 /REP_DB=WormBase Gene ID /WP=CE08421 /TR=Q93289 /GB=CAB02262.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070026(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070026 NCBI Caenorhabditis elegans C27D8.2 (C27D8.2) mRNA, complete cds. 11/11 None
SNAP00000003312 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12712168:12714717:1 11/11 None
GENEFINDER00000003315 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12712804:12714717:1 11/11 None
C27D8.2 ENSEMBL cdna:known chromosome:CEL140:IV:12712759:12714726:1 gene:C27D8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12712809-12714723(+) 94.88 100.0
Public Domain and Genome References
Gene Title
Ensembl C27D8.2
Entrez Gene 178225 Entrez gene
SwissProt Q93289 EMBL-EBI
WormBase CE08421 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02262 Hypothetical protein C27D8.2 [Caenorhabditis elegans] ref|NP_502427.1| C27D8.2 [Caenorhabditis elegans] 0.0
blast CAE66804 Hypothetical protein CBG12166 [Caenorhabditis briggsae] 3.0E-98
Sequence
Target Sequence 
>C. ELEGANS:178735_AT
catttaatgctcattcgtctcttcctctcagtcggccagcgttcaatatgaagatgaaaa
agtgcaaggagccgaaagccaagccaacttttgatccagccactgctcaacgagtcattg
atctgatcaacaccgacccgaaaactgtgaaaaccgaacgtagtgtcgagaaaaacaagt
tcaagaagaagcagaaggaggagaaaaaggcgaagaaacagatatcgaagagcacgaaga
aaaagtcaaagtgtgcttgtatagagtacgtcaagaacaagctcactgagaaaaagaaga
aaaatccattcaaggatccgaatgctgtgaaggttaagccgcctggaacgaatagcaaaa
tatccgaaacaagcaccaaatccacagcggtcatctccaaaatgtcttctctatcgaaaa
tccagcccacttcaactcttgagaaaaacctgcacaaacgaagaaccggcaagcgtacca
agatccaaggagctatcaagctttccaaatcacagtcggaacagaacattagtagctcga
agagcaaatcggcatcacgcagtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTTAATGCTCATTCGTCTCTTCC 285 215 905 Antisense
TCAGTCGGCCAGCGTTCAATATGAA 702 609 932 Antisense
AAGCCAAGCCAACTTTTGATCCAGC 202 149 980 Antisense
CAGCCACTGCTCAACGAGTCATTGA 292 203 1001 Antisense
GATCAACACCGACCCGAAAACTGTG 159 419 1029 Antisense
GAAGGTTAAGCCGCCTGGAACGAAT 556 317 1233 Antisense
GCGGTCATCTCCAAAATGTCTTCTC 397 293 1291 Antisense
CTTCTCTATCGAAAATCCAGCCCAC 232 221 1310 Antisense
AGAACCGGCAAGCGTACCAAGATCC 508 67 1366 Antisense
ATCAAGCTTTCCAAATCACAGTCGG 374 27 1399 Antisense
AGAGCAAATCGGCATCACGCAGTGG 657 67 1445 Antisense
Sequence Source Affymetrix Proprietary Database