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[178727_s_at]

Description F54D5.9 /REP_DB=WormBase Gene ID /WP=CE03413 /TR=Q20775 /GB=CAA91335.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 40      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 23.8
(-3)
A 		87.1
(0.7)
P 		127.6
(3)
P 		105.7
(1.7)
P 		104
B 17.8
(-3)
A 		112
(2.9)
M 		113.8
(3)
P 		76.5
(0.7)
A 		96
C 35.1
(-3)
A 		103.3
(0.4)
P 		156.8
(3)
P 		77.2
(-0.9)
P 		122
avr 25.6
(-3) 		100.8
(1.2) 		132.7
(3) 		86.5
(0.4) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 537
1 2 3 4 5 6 range
A 43.9
(-2.9)
A 		37.1
(-3)
A 		91.1
(-2.2)
P 		119.8
(-1.7)
P 		279.4
(0.8)
P 		420.9
(3)
P 		384
B 38.9
(-3)
A 		52.3
(-2.8)
A 		104.2
(-2.2)
P 		116.8
(-2.1)
P 		307.7
(0.2)
P 		540
(3)
P 		501
C 36.8
(-3)
A 		72.7
(-2.6)
A 		82.1
(-2.5)
A 		116.8
(-2.1)
P 		232.3
(-0.8)
P 		573.6
(3)
P 		537
avr 39.9
(-3) 		54
(-2.8) 		92.5
(-2.3) 		117.8
(-2) 		273.1
(-0) 		511.5
(3) 		472
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 8.7
(-3)
A 		18.9
(-0.5)
P 		33.4
(3)
P 		15.5
(-1.3)
P 		25
B 5.7
(-3)
A 		27.7
(3)
M 		26.4
(2.6)
P 		16.3
(-0.1)
A 		22
C 11.6
(-3)
A 		29.7
(-0.5)
P 		54.7
(3)
P 		13
(-2.8)
P 		43
avr 8.7
(-3) 		25.4
(0.4) 		38.2
(3) 		14.9
(-1.7) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 8.1
(-3)
A 		13.2
(-2.7)
A 		19.8
(-2.3)
P 		30.4
(-1.6)
P 		84.3
(1.8)
P 		102.5
(3)
P 		94
B 13.5
(-3)
A 		17.4
(-2.9)
A 		25.3
(-2.6)
P 		30.9
(-2.4)
P 		125
(0.6)
P 		201.4
(3)
P 		188
C 13.6
(-3)
A 		22.2
(-2.7)
A 		20.9
(-2.8)
A 		23.5
(-2.7)
P 		75.2
(-0.9)
P 		192
(3)
P 		178
avr 11.7
(-3) 		17.6
(-2.8) 		22
(-2.6) 		28.3
(-2.4) 		94.8
(0.2) 		165.3
(3) 		154
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178727_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3184
Sequence Type Exemplar sequence
Representative Public ID F54D5.9 NCBI
Target Description F54D5.9 /REP_DB=WormBase Gene ID /WP=CE03413 /TR=Q20775 /GB=CAA91335.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064066(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064066 NCBI Caenorhabditis elegans F54D5.9 (F54D5.9) mRNA, complete cds. 11/11 None
SNAP00000004665 ENSEMBL cdna:SNAP chromosome:CEL140:II:11542716:11544676:-1 11/11 None
GENEFINDER00000004685 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11542716:11544676:-1 11/11 None
F54D5.9 ENSEMBL cdna:known chromosome:CEL140:II:11542559:11544736:-1 gene:F54D5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11542712-11544673(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D5.9
Entrez Gene 174766 Entrez gene
SwissProt Q20775 EMBL-EBI
WormBase CE03413 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91335 Hypothetical protein F54D5.9 [Caenorhabditis elegans] ref|NP_496467.1| F54D5.9 [Caenorhabditis elegans] 0.0
blast CAE59479 Hypothetical protein CBG02863 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.2E-6
Sequence
Target Sequence 
>C. ELEGANS:178727_S_AT
acgttgctcaatggaatccgcgctcttcgcatcatgaataattccaagagttcggaccaa
atgtggagcgggaagaaactttatcagcttttcctccggatcattgaagtttgcgaagta
caaggcttttggtctgagacctcatcttctatggcgctggccattgattcttcaggattg
ctcagcgaatatcttgtcacctgtctcgatcctcgccgcgacgactatgaaactctccag
aatgaagccgcggagatggtgtgtctcgtacgcaaccatctttatcgttggtcggctgca
cctgccacgtttctcgccgaaccactgcaccacgcattcaatcatcttgcgcaactcgat
ggtcattacgacggttgctaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTTGCTCAATGGAATCCGCGCTC 706 93 1081 Antisense
CAGCTTTTCCTCCGGATCATTGAAG 590 203 1165 Antisense
GGTCTGAGACCTCATCTTCTATGGC 396 503 1211 Antisense
TGATTCTTCAGGATTGCTCAGCGAA 688 563 1245 Antisense
CAGCGAATATCTTGTCACCTGTCTC 386 203 1263 Antisense
CCGCGACGACTATGAAACTCTCCAG 65 263 1296 Antisense
GAAACTCTCCAGAATGAAGCCGCGG 584 357 1309 Antisense
GAGATGGTGTGTCTCGTACGCAACC 512 395 1333 Antisense
GCAACCATCTTTATCGTTGGTCGGC 174 317 1352 Antisense
CATTCAATCATCTTGCGCAACTCGA 572 215 1415 Antisense
GATGGTCATTACGACGGTTGCTACA 111 407 1438 Antisense
Sequence Source Affymetrix Proprietary Database