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[178721_at]

Description C25F9.4 /REP_DB=WormBase Gene ID /WP=CE17468 /TR=O62078 /GB=CAB03922.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 41      4grp_high_8_8 > 40      6grp_total > 52      4grp_total > 55      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 296
1 2 3 4 range
A 313.9
(3)
P 		128.1
(-1.3)
P 		144.8
(-0.9)
P 		54
(-3)
A 		260
B 317.8
(3)
P 		144.8
(-1.2)
P 		110.5
(-2)
P 		69.6
(-3)
P 		248
C 350.4
(3)
P 		150.8
(-1.4)
P 		160.1
(-1.2)
P 		75.6
(-3)
P 		275
avr 327.4
(3) 		141.2
(-1.3) 		138.5
(-1.3) 		66.4
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 100.7
(-2.1)
A 		196.9
(0.7)
P 		272.1
(3)
P 		207.2
(1.1)
P 		133.3
(-1.2)
P 		71.7
(-3)
P 		200
B 155.3
(-1.1)
P 		186.5
(-0.2)
P 		285.8
(3)
P 		140.5
(-1.6)
P 		121.7
(-2.2)
P 		97.1
(-3)
P 		189
C 130.6
(-2.4)
P 		240.7
(1.8)
P 		270.5
(3)
P 		208
(0.6)
P 		116
(-3)
P 		139.1
(-2.1)
P 		155
avr 128.9
(-2.1) 		208
(0.6) 		276.1
(3) 		185.2
(-0.1) 		123.7
(-2.3) 		102.6
(-3) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 114.3
(3)
P 		27.8
(-1.9)
P 		37.8
(-1.3)
P 		7.9
(-3)
A 		106
B 101.3
(3)
P 		35.8
(-1.5)
P 		25.6
(-2.3)
P 		14.8
(-3)
P 		87
C 116
(3)
P 		43.4
(-1.2)
P 		55.8
(-0.5)
P 		12.7
(-3)
P 		103
avr 110.5
(3) 		35.7
(-1.5) 		39.7
(-1.3) 		11.8
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 18.6
(-2.9)
A 		70
(3)
P 		59.3
(1.8)
P 		52.5
(1)
P 		40.2
(-0.4)
P 		17.4
(-3)
P 		53
B 54
(0.2)
P 		62.1
(1.7)
P 		69.4
(3)
P 		37.2
(-2.8)
P 		49.5
(-0.6)
P 		36.2
(-3)
P 		33
C 48.4
(-1.2)
P 		73.5
(3)
P 		68.8
(2.2)
P 		41.8
(-2.3)
P 		37.5
(-3)
P 		46.6
(-1.5)
P 		36
avr 40.3
(-1.8) 		68.5
(3) 		65.8
(2.5) 		43.8
(-1.2) 		42.4
(-1.5) 		33.4
(-3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178721_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13811
Sequence Type Exemplar sequence
Representative Public ID C25F9.4 NCBI
Target Description C25F9.4 /REP_DB=WormBase Gene ID /WP=CE17468 /TR=O62078 /GB=CAB03922.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075377(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075377 NCBI Caenorhabditis elegans C25F9.4 (C25F9.4) mRNA, complete cds. 10/11 None
SNAP00000028315 ENSEMBL cdna:SNAP chromosome:CEL140:V:19414214:19416454:-1 9/11 None
GENEFINDER00000028325 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19413926:19418267:-1 10/11 None
C25F9.4 ENSEMBL cdna:known chromosome:CEL140:V:19413926:19416454:-1 gene:C25F9.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075383 1/11 Cross Hyb Matching Probes A 
M04C3.2 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19413925-19415398(-) 100.0 100.0
chrV:19449205-19450359(-) 90.71 90.71
Public Domain and Genome References
Gene Title
Ensembl C25F9.4
Entrez Gene 182905 Entrez gene
SwissProt O62078 EMBL-EBI
WormBase CE33975 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03922 Hypothetical protein C25F9.4 [Caenorhabditis elegans] ref|NP_507778.3| C25F9.4 [Caenorhabditis elegans] 0.0
blast CAB07266 Hypothetical protein M04C3.2 [Caenorhabditis elegans] ref|NP_507784.2| M04C3.2 [Caenorhabditis elegans] 0.0
blast CAA21518 Hypothetical protein Y43F8B.14 [Caenorhabditis elegans] ref|NP_507785.1| Y43F8B.14 [Caenorhabditis elegans] 1.0E-173
Protein Domains
Database ID Description E-Value
Pfam IPR008154 EMBL-EBI Amyloid A4 extracellular region 5.4E-5
Sequence
Target Sequence 
>C. ELEGANS:178721_AT
agcaagttaccttgcgtttcccaagaagtcgtaagttgtataaaatctcgattcaattta
ctaaaattagcattttaaaatttgcagcgaatgattataaaaaacacataactaatgcaa
aaaaagaaaaaaacgaaaatgagcccaatatcagcaaattttgcaacaatatgttagccg
caatagtcagctacaccaaagagttctacatcaaacatttcaaaatttgtgcagaaagtg
atgaggatatagaagaaatgtatgattcattttgtgctggttttaaaaacacgtcggatg
tcggtcttattatgaaaatatgggtggacgcgcactttaacttttccaaaagcgagcaag
atcgttgtttcattgggaaaacagcaatgaaaaatattatcgaaaattttgagaaagcaa
tcaaaataattgagaaagctacgcaaacatctattgaggaaagctacaaccaaatgagag
ctgctaggagtggaaaacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAAGTTACCTTGCGTTTCCCAAG 589 75 707 Antisense
TTGCGTTTCCCAAGAAGTCGTAAGT 686 607 718 Antisense
CAATATGTTAGCCGCAATAGTCAGC 423 179 873 Antisense
AATAGTCAGCTACACCAAAGAGTTC 582 165 888 Antisense
AAAACACGTCGGATGTCGGTCTTAT 511 129 992 Antisense
GTCGGATGTCGGTCTTATTATGAAA 576 471 999 Antisense
ATTATGAAAATATGGGTGGACGCGC 219 7 1015 Antisense
GTGGACGCGCACTTTAACTTTTCCA 555 481 1030 Antisense
GCGCACTTTAACTTTTCCAAAAGCG 333 287 1036 Antisense
GCGAGCAAGATCGTTGTTTCATTGG 67 291 1058 Antisense
GAGAGCTGCTAGGAGTGGAAAACGA 197 395 1182 Antisense
Sequence Source Affymetrix Proprietary Database