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[178711_at]

Description T22G5.1 /REP_DB=WormBase Gene ID /WP=CE13982 /TR=O02322 /GB=CAB03386.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 92      4grp_total > 11      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 20.7
(0.5)
A 		29.8
(3)
A 		8.3
(-3)
A 		21.2
(0.6)
A 		22
B 6.4
(-2.9)
A 		8.5
(-2.6)
A 		6
(-3)
A 		42.1
(3)
A 		36
C 8.1
(-2.6)
A 		6.3
(-2.8)
A 		4.4
(-3)
A 		67
(3)
A 		63
avr 11.7
(-2.1) 		14.9
(-1.6) 		6.2
(-3) 		43.4
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 9.2
(-2.3)
A 		2.8
(-3)
A 		33
(0.1)
A 		5.9
(-2.7)
A 		60.8
(3)
A 		23.3
(-0.9)
A 		58
B 9
(-1.5)
A 		24.6
(3)
A 		3.9
(-3)
A 		10.3
(-1.1)
A 		4.8
(-2.7)
A 		4.3
(-2.9)
A 		21
C 25.7
(1.4)
A 		33.9
(3)
A 		15.5
(-0.7)
A 		27.9
(1.8)
A 		4.9
(-2.8)
A 		3.7
(-3)
A 		30
avr 14.6
(-1.1) 		20.4
(1.6) 		17.5
(0.3) 		14.7
(-1) 		23.5
(3) 		10.4
(-3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 7.5
(3)
A 		6.5
(1.9)
A 		2.2
(-3)
A 		3.1
(-2)
A 		5
B 2
(-2.5)
A 		2.1
(-2.4)
A 		1.4
(-3)
A 		9
(3)
A 		8
C 2.7
(-2.3)
A 		1.8
(-2.8)
A 		1.5
(-3)
A 		11.3
(3)
A 		10
avr 4.1
(-0.6) 		3.5
(-1.2) 		1.7
(-3) 		7.8
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 1.7
(-2.8)
A 		1
(-3)
A 		7.2
(-0.9)
A 		1.5
(-2.8)
A 		18.4
(3)
A 		5.7
(-1.4)
A 		17
B 3.1
(-1.3)
A 		8.2
(3)
A 		1
(-3)
A 		2.7
(-1.6)
A 		2
(-2.2)
A 		1.6
(-2.5)
A 		7
C 9.5
(2.5)
A 		10.3
(3)
A 		3.9
(-1.2)
A 		5.6
(-0.1)
A 		1.6
(-2.7)
A 		1.2
(-3)
A 		9
avr 4.8
(-0.3) 		6.5
(1.9) 		4
(-1.4) 		3.3
(-2.3) 		7.3
(3) 		2.8
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178711_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14293
Sequence Type Exemplar sequence
Representative Public ID T22G5.1 NCBI
Target Description T22G5.1 /REP_DB=WormBase Gene ID /WP=CE13982 /TR=O02322 /GB=CAB03386.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074038(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074038 NCBI Caenorhabditis elegans T22G5.1 (T22G5.1) mRNA, complete cds. 11/11 None
SNAP00000016893 ENSEMBL cdna:SNAP chromosome:CEL140:V:13879426:13880538:1 11/11 None
GENEFINDER00000016907 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13877880:13880538:1 11/11 None
T22G5.1 ENSEMBL cdna:known chromosome:CEL140:V:13879426:13880538:1 gene:T22G5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13879425-13880538(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22G5.1
Entrez Gene 179882 Entrez gene
SwissProt O02322 EMBL-EBI
WormBase CE13982 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6071 glycerol metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8889 glycerophosphodiester phosphodiesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03386 Hypothetical protein T22G5.1 [Caenorhabditis elegans] ref|NP_506439.1| T22G5.1 [Caenorhabditis elegans] 1.0E-161
blast CAB03386 Hypothetical protein T22G5.1 [Caenorhabditis elegans] ref|NP_506439.1| T22G5.1 [Caenorhabditis elegans] 1.0E-160
blast AAB52706 Hypothetical protein ZC155.4 [Caenorhabditis elegans] ref|NP_498106.1| ZC155.4 [Caenorhabditis elegans] 1.0E-141
blast CAE56305 Hypothetical protein CBG23963 [Caenorhabditis briggsae] 1.0E-138
Protein Domains
Database ID Description E-Value
Pfam IPR004129 EMBL-EBI Glycerophosphoryl diester phosphodiesterase 6.4E-66
Pfam IPR004129 EMBL-EBI Glycerophosphoryl diester phosphodiesterase 6.4E-66
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 3.7E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506439.1 2 5-24,31-50
T22G5.1 2 5-24,31-50
SNAP00000016893 2 5-24,31-50
GENEFINDER00000016907 2 190-212,219-238
Sequence
Target Sequence 
>C. ELEGANS:178711_AT
gacagtaacacagtccgcgtaaccggagttgacaaagaaatttctgcaatgaatataact
gaattccgtaaactccgattcattggcacaaatgtttcccttccaactttcgaagaagcc
gttgctcactgtgttgccaacaacatcatgatgatttgggatgtgaagaatgttgatgaa
aacttgctcaaacagtttgtcattcaaatcagaacttacaacctttactccaaggttctt
gtgtctggattcaatccgattgacacatatcttgtgaaaatggcggatccaaagattttg
acagggttcacatggagaagttgggagttgagtacaactgatgaagcagcgacgactcca
cgattcactggaacccttcacgctgtcgctagtgtccttgacgttctcgcatttggtttg
gctcgttcgttgataattccgaaattcctcggatccgatgtgattttctatcacgttagc
gatatcagcaggttcctaaaaaccgatgcagcagccaacaacatttaccttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGTAACACAGTCCGCGTAACCG 154 367 361 Antisense
AATTCCGTAAACTCCGATTCATTGG 157 167 422 Antisense
TTGGCACAAATGTTTCCCTTCCAAC 340 707 443 Antisense
CGAAGAAGCCGTTGCTCACTGTGTT 165 253 471 Antisense
TCAGAACTTACAACCTTTACTCCAA 479 621 569 Antisense
TGATGAAGCAGCGACGACTCCACGA 667 565 699 Antisense
GACGACTCCACGATTCACTGGAACC 193 377 711 Antisense
GACGTTCTCGCATTTGGTTTGGCTC 18 379 760 Antisense
GGTTTGGCTCGTTCGTTGATAATTC 198 505 775 Antisense
GTTCCTAAAAACCGATGCAGCAGCC 471 439 852 Antisense
AGCAGCCAACAACATTTACCTTGCC 215 77 870 Antisense
Sequence Source Affymetrix Proprietary Database