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[178682_at]

Description W01G7.5 /REP_DB=WormBase Gene ID /WP=CE20129 /TR=Q9XTB5 /GB=CAA21599.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 84      4grp_total > 19      6grp_cgc > 77      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 23.9
(-2.1)
A 		41.2
(-0.7)
A 		11.7
(-3)
A 		90.1
(3)
A 		78
B 48.6
(-0.4)
P 		64.4
(1.5)
A 		27.6
(-3)
A 		77
(3)
P 		49
C 25.7
(-3)
A 		52.6
(2.3)
A 		31.8
(-1.8)
A 		56
(3)
A 		30
avr 32.7
(-1.9) 		52.7
(0.4) 		23.7
(-3) 		74.4
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 4.9
(-3)
A 		20.4
(0.5)
A 		7.8
(-2.4)
A 		15.9
(-0.5)
A 		19.9
(0.3)
A 		31.8
(3)
A 		27
B 10.9
(-2.6)
A 		32.1
(3)
A 		21.4
(0.2)
A 		16.1
(-1.2)
A 		9.3
(-3)
A 		21.9
(0.3)
A 		23
C 2.9
(-3)
A 		11.5
(-1.9)
A 		16
(-1.4)
A 		51.7
(3)
A 		36.6
(1.1)
A 		15.7
(-1.4)
A 		49
avr 6.2
(-3) 		21.3
(1.2) 		15.1
(-0.5) 		27.9
(3) 		21.9
(1.3) 		23.1
(1.7) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 8.7
(0.4)
A 		9
(0.5)
A 		3
(-3)
A 		13.2
(3)
A 		10
B 15.5
(2.5)
P 		15.9
(2.7)
A 		6.4
(-3)
A 		16.4
(3)
P 		10
C 8.5
(-3)
A 		15.1
(3)
A 		11.1
(-0.6)
A 		9.4
(-2.2)
A 		7
avr 10.9
(0.8) 		13.3
(3) 		6.8
(-3) 		13
(2.7) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.9
(-3)
A 		7.3
(2.6)
A 		1.7
(-2.3)
A 		4
(-0.3)
A 		6
(1.5)
A 		7.7
(3)
A 		7
B 3.8
(-3)
A 		10.7
(3)
A 		5.2
(-1.8)
A 		4.3
(-2.6)
A 		3.8
(-3)
A 		8.2
(0.8)
A 		7
C 1.1
(-3)
A 		3.5
(-1.7)
A 		4.1
(-1.3)
A 		10.4
(2.2)
A 		11.9
(3)
A 		5.3
(-0.7)
A 		11
avr 1.9
(-3) 		7.2
(3) 		3.7
(-1) 		6.2
(1.9) 		7.2
(3) 		7.1
(2.9) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178682_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3332
Sequence Type Exemplar sequence
Representative Public ID W01G7.5 NCBI
Target Description W01G7.5 /REP_DB=WormBase Gene ID /WP=CE20129 /TR=Q9XTB5 /GB=CAA21599.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064543(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064543 NCBI Caenorhabditis elegans LEM domain protein family member (lem-2) (lem-2) mRNA, complete cds. 11/11 None
SNAP00000006825 ENSEMBL cdna:SNAP chromosome:CEL140:II:14063800:14091200:1 11/11 None
W01G7.5 ENSEMBL cdna:known chromosome:CEL140:II:14063346:14066061:1 gene:W01G7.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000006834 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14063342-14065746(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lem-2
Ensembl W01G7.5
Entrez Gene 175058 Entrez gene
SwissProt Q9XTB5 EMBL-EBI
WormBase CE20129 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5635 nuclear membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC35856 Hypothetical protein Y39G8C.1 [Caenorhabditis elegans] ref|NP_496945.2| XRN (mouse/S. cerevisiae) ribonuclease related family member (xrn-1) [Caenorhabditis elegans] 0.0
blast T26774 hypothetical protein Y39G8C.b - Caenorhabditis elegans 0.0
blast CAE73396 Hypothetical protein CBG20837 [Caenorhabditis briggsae] 0.0
blast CAB03457 Hypothetical protein W01G7.5 [Caenorhabditis elegans] emb|CAA21599.1| Hypothetical protein W01G7.5 [Caenorhabditis elegans] ref|NP_496944.1| LEM domain protein family member (lem-2) [Caenorhabditis elegans] sp|Q9XTB5|LEM2_CAEEL LEM protein 2 (Ce-MAN1) (MAN1 homolog) 1.0E-177
blast CAE73395 Hypothetical protein CBG20836 [Caenorhabditis briggsae] 1.0E-143
blast T26773 hypothetical protein Y39G8C.a - Caenorhabditis elegans 1.0E-39
Protein Domains
Database ID Description E-Value
scop a.140.1.LEM domain All alpha proteins; LEM/SAP HeH motif; LEM domain; LEM domain 0.0149999996647239
Pfam IPR004859 EMBL-EBI Putative 5-3 exonuclease domain 2.3E-78
Pfam IPR003887 EMBL-EBI Lamino-associated polypeptide 2/emerin 1.4E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496944.1 2 325-347,378-400
W01G7.5 2 325-347,378-400
SNAP00000006825 2 193-215,242-264
Sequence
Target Sequence 
>C. ELEGANS:178682_AT
gcctccagaatccgtgtgcagaagaatccgagaaccgggaaggtgactgtgaagcagacg
aatattttcaacgaggcgatttatttcgcgctctatgtgattttaattttgttcgtcgtg
cttggaatcgcctacgcgctgacaacaacgcatcgcccgaaaactgcggatttctcgggt
tattggggagttttgaaggcggccggccgagattcgctcaatttcttctacaattacgcg
attcttccagtcgtttcactggggattttcgtcgttctcggagccggaatctactttgga
caccgcaaatacaaggaggccaaggagcaggaagaggcgaagctctacgagctcatcgag
cgcatcaccgagctcatccgcgagtcctcaattgacggcgatccgtacgtctctcagcca
cacgtccgcgatgtcctcttcccgccggcaaagagacgctcggctgagctggctcgctgg
gagcaagcggtcaagttcatcgacaccaatgagtctcgcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCTCCAGAATCCGTGTGCAGAAGA 381 283 910 Antisense
GATTTATTTCGCGCTCTATGTGATT 270 429 987 Antisense
CTACGCGCTGACAACAACGCATCGC 97 227 1041 Antisense
AGATTCGCTCAATTTCTTCTACAAT 696 65 1119 Antisense
AATTACGCGATTCTTCCAGTCGTTT 557 171 1141 Antisense
TTTCACTGGGGATTTTCGTCGTTCT 141 667 1163 Antisense
CGGAGCCGGAATCTACTTTGGACAC 68 243 1188 Antisense
AGCTCTACGAGCTCATCGAGCGCAT 91 81 1250 Antisense
GCGAGTCCTCAATTGACGGCGATCC 582 289 1289 Antisense
GCAAGCGGTCAAGTTCATCGACACC 680 319 1392 Antisense
CATCGACACCAATGAGTCTCGCGTC 96 213 1407 Antisense
Sequence Source Affymetrix Proprietary Database