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[178674_at]

Description Y6B3B.9 /REP_DB=WormBase Gene ID /WP=CE20183 /TR=Q9U1T1 /GB=CAB63413.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 28      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 80      6grp_total > 17      4grp_total > 29      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 316.2
(3)
P 		246.2
(-2.8)
P 		244.3
(-3)
P 		313.4
(2.8)
P 		72
B 266.7
(2.3)
P 		273.8
(3)
P 		227
(-1.4)
P 		209.5
(-3)
P 		64
C 249.5
(1.7)
P 		268.9
(3)
P 		180.5
(-3)
P 		263.4
(2.6)
P 		88
avr 277.5
(3) 		263
(1.6) 		217.3
(-3) 		262.1
(1.5) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 209.7
(3)
P 		188.7
(0.9)
P 		186
(0.6)
P 		163.5
(-1.6)
P 		149.5
(-3)
P 		168.2
(-1.1)
P 		60
B 262.7
(3)
P 		171.9
(-2)
P 		170.6
(-2.1)
P 		154.8
(-3)
P 		181.4
(-1.5)
P 		192.2
(-0.9)
P 		108
C 224
(3)
P 		184.2
(-0.3)
P 		174.8
(-1.1)
P 		155.6
(-2.7)
P 		193
(0.4)
P 		152.4
(-3)
P 		72
avr 232.1
(3) 		181.6
(-1.1) 		177.1
(-1.5) 		158
(-3) 		174.6
(-1.7) 		170.9
(-2) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 115.1
(3)
P 		53.4
(-2.3)
P 		63.9
(-1.4)
P 		45.9
(-3)
P 		69
B 85.1
(3)
P 		67.6
(0.4)
P 		52.6
(-1.8)
P 		44.6
(-3)
P 		41
C 82.6
(3)
P 		77.3
(2.2)
P 		62.9
(-0.1)
P 		44.4
(-3)
P 		38
avr 94.3
(3) 		66.1
(-0.4) 		59.8
(-1.2) 		45
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 38.7
(-3)
P 		67.1
(3)
P 		40.5
(-2.6)
P 		41.5
(-2.4)
P 		45.1
(-1.6)
P 		41
(-2.5)
P 		28
B 91.4
(3)
P 		57.2
(-1.1)
P 		41.4
(-3)
P 		41
(-3)
P 		73.7
(0.9)
P 		71.7
(0.7)
P 		50
C 83
(3)
P 		56.2
(-0.1)
P 		44.5
(-1.5)
P 		31.3
(-3)
P 		62.4
(0.6)
P 		51
(-0.7)
P 		52
avr 71
(3) 		60.2
(1) 		42.1
(-2.2) 		37.9
(-3) 		60.4
(1.1) 		54.6
(0) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178674_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1183
Sequence Type Exemplar sequence
Representative Public ID Y6B3B.9 NCBI
Target Description Y6B3B.9 /REP_DB=WormBase Gene ID /WP=CE20183 /TR=Q9U1T1 /GB=CAB63413.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061003(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061003 NCBI Caenorhabditis elegans Y6B3B.9 (Y6B3B.9) mRNA, complete cds. 11/11 None
SNAP00000043749 ENSEMBL cdna:SNAP chromosome:CEL140:I:13743340:13747653:-1 11/11 None
GENEFINDER00000043762 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13743538:13747653:-1 10/11 None
Y6B3B.9 ENSEMBL cdna:known chromosome:CEL140:I:13743087:13759242:-1 gene:Y6B3B.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000043764 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13743270-13747584(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y6B3B.9
Entrez Gene 173239 Entrez gene
SwissProt Q9U1T1 EMBL-EBI
WormBase CE34133 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63413 Hypothetical protein Y6B3B.9 [Caenorhabditis elegans] ref|NP_493404.2| Y6B3B.9 [Caenorhabditis elegans] 0.0
blast CAB63413 Hypothetical protein Y6B3B.9 [Caenorhabditis elegans] ref|NP_493404.2| Y6B3B.9 [Caenorhabditis elegans] 1.0E-160
blast CAB63413 Hypothetical protein Y6B3B.9 [Caenorhabditis elegans] ref|NP_493404.2| Y6B3B.9 [Caenorhabditis elegans] 1.0E-138
blast CAE68021 Hypothetical protein CBG13636 [Caenorhabditis briggsae] 7.0E-81
blast CAE68021 Hypothetical protein CBG13636 [Caenorhabditis briggsae] 1.0E-80
blast CAE68021 Hypothetical protein CBG13636 [Caenorhabditis briggsae] 7.0E-66
blast NP_112483 LAS1-like [Homo sapiens] emb|CAI42835.1| novel protein [Homo sapiens] dbj|BAB14114.1| unnamed protein product [Homo sapiens] gb|AAO15306.1| MSTP060 [Homo sapiens] sp|Q9Y4W2|LAS1L_HUMAN LAS1-like protein gb|AAH14545.1| LAS1-like [Homo sapiens] 2.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR007174 EMBL-EBI Las1-like 4.6E-13
Sequence
Target Sequence 
>C. ELEGANS:178674_AT
gcattctgggagccactgtttcatgtgatgttcagtttgaagttgggagccgaaattgtt
gtcgctttcctttggagacttcgagaaccgatgattaaggatatgacacgtcatcagata
actgcatatgttcgaatgattgttcagcagtttgccgacgccgatgtgttctccgatgag
gaatggtataaagtgctcgatcatcttcttccagttgccaagaatttcagcacagagttc
attgagctcgtaatggcccgctgcacaacactggctcacaagcggaaaaagcaaattcgt
caaatattaaacatctccacaaaaagccacgttgaatcaccatctacatcatcatccgac
actgtgcacactgtttcagatcttcaaaaattgctgaaaaaatcgtcgaaaagtgtagtg
aaatctgaaccggtgacatcgaatgctgatggaaatcgtactggaattgagttgtgtgac
gtcgcagcttgggctgaagtaccttttggaatggcaccaggacaacgtatcgaaacattt
accgtcgttttggatcatcatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATTCTGGGAGCCACTGTTTCATG 500 307 343 Antisense
GGAGCCGAAATTGTTGTCGCTTTCC 140 519 388 Antisense
GTCGCTTTCCTTTGGAGACTTCGAG 273 473 403 Antisense
ATAAAGTGCTCGATCATCTTCTTCC 199 25 530 Antisense
TGCACAACACTGGCTCACAAGCGGA 246 577 604 Antisense
AGCCACGTTGAATCACCATCTACAT 531 83 667 Antisense
TCTACATCATCATCCGACACTGTGC 534 613 685 Antisense
GACACTGTGCACACTGTTTCAGATC 540 367 700 Antisense
ATTGAGTTGTGTGACGTCGCAGCTT 247 5 808 Antisense
GCAGCTTGGGCTGAAGTACCTTTTG 565 269 826 Antisense
TTACCGTCGTTTTGGATCATCATGG 11 683 881 Antisense
Sequence Source Affymetrix Proprietary Database