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[178669_s_at]

Description ZC334.3 /REP_DB=WormBase Gene ID /WP=CE24702 /TR=Q9U1P6 /GB=CAB04964.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 90      6grp_total > 64      4grp_total > 03      6grp_cgc > 66      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 723
1 2 3 4 range
A 109.5
(-2.9)
P 		125.5
(-2.8)
P 		103.3
(-3)
P 		734.5
(3)
P 		631
B 166.9
(-2.2)
P 		90.4
(-2.9)
P 		80.3
(-3)
P 		692.5
(3)
P 		612
C 146.1
(-2.4)
P 		81
(-3)
P 		75.2
(-3)
P 		798.2
(3)
P 		723
avr 140.8
(-2.5) 		99
(-2.9) 		86.3
(-3) 		741.7
(3) 		655

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 90
(-3)
P 		162
(0.2)
P 		204.5
(2.1)
P 		225.8
(3)
P 		119.2
(-1.7)
P 		190.8
(1.5)
P 		136
B 114.2
(-3)
P 		125.7
(-2.5)
P 		249
(3)
P 		162.9
(-0.8)
P 		127.7
(-2.4)
P 		197.2
(0.7)
P 		135
C 112.5
(-2.9)
P 		109.4
(-3)
P 		237.7
(3)
P 		221.2
(2.2)
P 		203
(1.4)
P 		221.4
(2.2)
P 		128
avr 105.6
(-3) 		132.4
(-1.7) 		230.4
(3) 		203.3
(1.7) 		150
(-0.9) 		203.1
(1.7) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 39.9
(-2)
P 		27.2
(-3)
P 		27
(-3)
P 		107.5
(3)
P 		81
B 53.2
(-1.4)
P 		22.3
(-2.8)
P 		18.6
(-3)
P 		147.6
(3)
P 		129
C 48.4
(-1.6)
P 		23.3
(-3)
P 		26.2
(-2.8)
P 		134.6
(3)
P 		111
avr 47.2
(-1.7) 		24.3
(-3) 		23.9
(-3) 		129.9
(3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 16.6
(-3)
P 		57.6
(3)
P 		44.5
(1.1)
P 		57.3
(3)
P 		36
(-0.2)
P 		46.5
(1.4)
P 		41
B 39.7
(-3)
P 		41.8
(-2.6)
P 		60.5
(0.7)
P 		43.1
(-2.4)
P 		51.9
(-0.8)
P 		73.5
(3)
P 		34
C 41.7
(-1.8)
P 		33.4
(-3)
P 		60.5
(1)
P 		44.5
(-1.4)
P 		65.7
(1.8)
P 		74.1
(3)
P 		41
avr 32.7
(-3) 		44.3
(-0.8) 		55.2
(1.2) 		48.3
(-0.1) 		51.2
(0.5) 		64.7
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178669_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1255
Sequence Type Exemplar sequence
Representative Public ID ZC334.3 NCBI
Target Description ZC334.3 /REP_DB=WormBase Gene ID /WP=CE24702 /TR=Q9U1P6 /GB=CAB04964.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061042(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061042 NCBI Caenorhabditis elegans INSulin related family member (ins-24) (ins-24) mRNA, complete cds. 11/11 A 
SNAP00000000603 ENSEMBL cdna:SNAP chromosome:CEL140:I:14039418:14039772:-1 11/11 None
GENEFINDER00000000608 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14039418:14039838:-1 11/11 None
ZC334.3 ENSEMBL cdna:known chromosome:CEL140:I:14039333:14039772:-1 gene:ZC334.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14039348-14039769(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-24
Ensembl ZC334.3
Entrez Gene 173266 Entrez gene
SwissProt Q9U1P6 EMBL-EBI
WormBase CE36062 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04964 Hypothetical protein ZC334.3 [Caenorhabditis elegans] ref|NP_493443.2| INSulin related family member (ins-24) [Caenorhabditis elegans] 8.0E-47
blast CAB04963 Hypothetical protein ZC334.2 [Caenorhabditis elegans] ref|NP_493444.1| INSulin related family member (ins-30) [Caenorhabditis elegans] 1.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.0E-30
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 1.0E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000000608 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:178669_S_AT
aaagcctcaaggagatgggactcattcgagccaaccagggacctcaaaaagcgtgcggac
gatcaatgatgatgaaggtgcagaagctttgcgcgggcggatgcacaattcagaacgacg
atcttaccatcaaatcctgcagtactgggtacaccgatgccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCCTCAAGGAGATGGGACTCAT 2 125 188 Antisense
GAGATGGGACTCATTCGAGCCAACC 320 395 199 Antisense
TTCGAGCCAACCAGGGACCTCAAAA 469 683 212 Antisense
GGGACCTCAAAAAGCGTGCGGACGA 18 495 225 Antisense
CAAAAAGCGTGCGGACGATCAATGA 514 189 232 Antisense
GAAGGTGCAGAAGCTTTGCGCGGGC 296 335 261 Antisense
AGCTTTGCGCGGGCGGATGCACAAT 425 79 272 Antisense
GCGGGCGGATGCACAATTCAGAACG 646 291 280 Antisense
ATTCAGAACGACGATCTTACCATCA 632 9 295 Antisense
CTTACCATCAAATCCTGCAGTACTG 6 223 310 Antisense
TGCAGTACTGGGTACACCGATGCCG 282 577 325 Antisense
Sequence Source Affymetrix Proprietary Database