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[178664_at]

Description F21D5.8 /REP_DB=WormBase Gene ID /WP=CE03250 /TR=Q19685 /GB=CAA91037.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 21      4grp_highest_8_8 > 12      4grp_high_8_8 > 31      6grp_highest_nonorm > 66      6grp_total > 41      4grp_total > 32      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 905
1 2 3 4 range
A 1346.1
(-3)
P 		1905.5
(1.8)
P 		2045.1
(3)
P 		1941.3
(2.1)
P 		699
B 1444.2
(-3)
P 		1622.7
(-1.5)
P 		2166.9
(3)
P 		2063.6
(2.1)
P 		723
C 1506.1
(-3)
P 		1908.2
(0.2)
P 		1959.7
(0.7)
P 		2251.5
(3)
P 		745
avr 1432.1
(-3) 		1812.1
(0.5) 		2057.2
(2.7) 		2085.5
(3) 		653

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1113
1 2 3 4 5 6 range
A 1416.6
(-0)
P 		1349.5
(-0.4)
P 		1978.6
(3)
P 		1669.6
(1.3)
P 		916.4
(-2.7)
P 		865.9
(-3)
P 		1113
B 1239.9
(-0.9)
P 		1669
(3)
P 		1627.5
(2.6)
P 		1250.3
(-0.8)
P 		1384.7
(0.4)
P 		1004.6
(-3)
P 		664
C 1532.2
(2.4)
P 		1477
(1.7)
P 		1212.3
(-1.6)
P 		1578
(3)
P 		1098.1
(-3)
P 		1100.7
(-3)
P 		480
avr 1396.2
(1) 		1498.5
(2) 		1606.1
(3) 		1499.3
(2) 		1133.1
(-1.6) 		990.4
(-3) 		616
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 399
1 2 3 4 range
A 490.1
(1.9)
P 		413.6
(0.1)
P 		534.6
(3)
P 		284.2
(-3)
P 		250
B 460.5
(0.5)
P 		400.8
(-3)
P 		502.1
(3)
P 		439.8
(-0.7)
P 		101
C 498.6
(-0.6)
P 		548.6
(0.3)
P 		683
(3)
P 		379.6
(-3)
P 		303
avr 483.1
(0.4) 		454.3
(-0.5) 		573.2
(3) 		367.9
(-3) 		205

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 357
1 2 3 4 5 6 range
A 261.1
(-1.9)
P 		479.9
(3)
P 		430.9
(1.9)
P 		423.4
(1.7)
P 		276.5
(-1.5)
P 		210.8
(-3)
P 		269
B 431.4
(-0.4)
P 		555.6
(2.8)
P 		395.2
(-1.3)
P 		331.2
(-3)
P 		562.6
(3)
P 		374.6
(-1.9)
P 		231
C 567.9
(3)
P 		450.7
(0.3)
P 		308.5
(-3)
P 		317.3
(-2.8)
P 		355.3
(-1.9)
P 		368.5
(-1.6)
P 		259
avr 420.1
(0.5) 		495.4
(3) 		378.2
(-1) 		357.3
(-1.7) 		398.1
(-0.3) 		318
(-3) 		177
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178664_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10195
Sequence Type Exemplar sequence
Representative Public ID F21D5.8 NCBI
Target Description F21D5.8 /REP_DB=WormBase Gene ID /WP=CE03250 /TR=Q19685 /GB=CAA91037.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069104(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069104 NCBI Caenorhabditis elegans F21D5.8 (F21D5.8) mRNA, complete cds. 11/11 None
SNAP00000028042 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8739577:8740008:-1 11/11 None
GENEFINDER00000028052 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8739086:8740008:-1 11/11 None
F21D5.8 ENSEMBL cdna:known chromosome:CEL140:IV:8739577:8740008:-1 gene:F21D5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8739582-8740014(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21D5.8
Entrez Gene 184770 Entrez gene
SwissProt Q19685 EMBL-EBI
WormBase CE03250 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91037 Hypothetical protein F21D5.8 [Caenorhabditis elegans] ref|NP_501505.1| F21D5.8 [Caenorhabditis elegans] 3.0E-68
blast CAA91037 Hypothetical protein F21D5.8 [Caenorhabditis elegans] ref|NP_501505.1| F21D5.8 [Caenorhabditis elegans] 1.0E-67
blast CAE70019 Hypothetical protein CBG16434 [Caenorhabditis briggsae] 5.0E-65
blast CAE70019 Hypothetical protein CBG16434 [Caenorhabditis briggsae] 2.0E-64
blast CAA91035 Hypothetical protein F21D5.5 [Caenorhabditis elegans] ref|NP_501503.1| F21D5.5 [Caenorhabditis elegans] gb|AAG41142.1| polynucleotide kinase 3' phosphatase [Caenorhabditis elegans] sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV 7.0E-27
blast XP_216135 PREDICTED: similar to Mitochondrial 28S ribosomal protein S33 (S33mt) (MRP-S33) (Ganglioside-induced differentiation-associated-protein 3) [Rattus norvegicus] 1.0E-14
Sequence
Target Sequence 
>C. ELEGANS:178664_AT
cacgtctggctgcatcacgaattgtgcacgccggccgcggaatctctcaacctacaccat
tcggaaaacgaatggatcgattgtcaaatcgagtatggggagaggttgtcatgccaactg
acactaaaagtttaaaggttgtccgagtaatgtctgcagaaccatatgaaaccaaagaac
aattatcaccgaaatactatccaaatcttccgatgtttcattatttgaccaaaatgctac
gattccatggactattttttgatgatcacgtagttttcagagatgttcaagataatttga
aaattattcgcggaaaagttgttcggccacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGTCTGGCTGCATCACGAATTGT 165 199 20 Antisense
TGCATCACGAATTGTGCACGCCGGC 454 577 30 Antisense
TCTCTCAACCTACACCATTCGGAAA 40 611 62 Antisense
GGGAGAGGTTGTCATGCCAACTGAC 418 495 117 Antisense
AGGTTGTCCGAGTAATGTCTGCAGA 380 57 155 Antisense
TGTCTGCAGAACCATATGAAACCAA 700 557 170 Antisense
AATACTATCCAAATCTTCCGATGTT 230 173 212 Antisense
TTCCGATGTTTCATTATTTGACCAA 693 697 227 Antisense
AAAATGCTACGATTCCATGGACTAT 472 133 250 Antisense
ATGGACTATTTTTTGATGATCACGT 86 53 266 Antisense
TCGCGGAAAAGTTGTTCGGCCACCA 684 599 327 Antisense
Sequence Source Affymetrix Proprietary Database