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[178663_at]

Description T16G1.8 /REP_DB=WormBase Gene ID /WP=CE20078 /TR=Q9XUP5 /GB=CAB04730.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 11      4grp_total > 49      6grp_cgc > 44      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 7.2
(-3)
A 		203.9
(3)
A 		169.1
(1.9)
A 		15.8
(-2.7)
A 		197
B 14.6
(-3)
A 		191.7
(3)
A 		147.9
(1.5)
A 		67.7
(-1.2)
A 		177
C 20.3
(-3)
A 		155.2
(1.8)
A 		187.7
(3)
A 		60.8
(-1.5)
A 		167
avr 14
(-3) 		183.6
(3) 		168.2
(2.5) 		48.1
(-1.8) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 86.4
(-0.6)
A 		143.9
(3)
A 		132.4
(2.3)
A 		121
(1.6)
A 		47.2
(-3)
A 		59.5
(-2.2)
A 		97
B 65.1
(-0.8)
A 		144
(3)
A 		100.7
(0.9)
A 		18.9
(-3)
A 		86.2
(0.2)
A 		38
(-2.1)
A 		125
C 89.9
(-0.3)
A 		148.2
(3)
A 		104.5
(0.5)
A 		41.8
(-3)
A 		84.2
(-0.6)
A 		53.3
(-2.4)
A 		106
avr 80.5
(-1.1) 		145.4
(3) 		112.5
(0.9) 		60.6
(-2.4) 		72.5
(-1.6) 		50.3
(-3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 2.6
(-3)
A 		44.3
(3)
A 		44.2
(3)
A 		2.3
(-3)
A 		42
B 4.7
(-3)
A 		47.3
(3)
A 		34.3
(1.2)
A 		14.4
(-1.6)
A 		43
C 6.7
(-3)
A 		44.6
(0.9)
A 		65.4
(3)
A 		10.2
(-2.6)
A 		59
avr 4.7
(-3) 		45.4
(2.6) 		48
(3) 		9
(-2.4) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 15.9
(-2.7)
A 		51.2
(3)
A 		28.8
(-0.6)
A 		30.7
(-0.3)
A 		14.3
(-3)
A 		14.5
(-3)
A 		37
B 22.6
(-0.5)
A 		47.9
(3)
A 		24.4
(-0.3)
A 		5
(-3)
A 		35
(1.2)
A 		14.2
(-1.7)
A 		43
C 33.3
(1.1)
A 		45.2
(3)
A 		26.6
(-0)
A 		8.4
(-3)
A 		27.2
(0.1)
A 		17.8
(-1.5)
A 		37
avr 23.9
(-1.3) 		48.1
(3) 		26.6
(-0.9) 		14.7
(-3) 		25.5
(-1.1) 		15.5
(-2.9) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178663_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13098
Sequence Type Exemplar sequence
Representative Public ID T16G1.8 NCBI
Target Description T16G1.8 /REP_DB=WormBase Gene ID /WP=CE20078 /TR=Q9XUP5 /GB=CAB04730.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073837(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073837 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-2) (grl-2) mRNA, complete cds. 11/11 None
SNAP00000020371 ENSEMBL cdna:SNAP chromosome:CEL140:V:12947768:12949380:1 11/11 None
GENEFINDER00000020385 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12947768:12949380:1 11/11 None
T16G1.8 ENSEMBL cdna:known chromosome:CEL140:V:12947451:12949443:1 gene:T16G1.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12947767-12949380(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-2
Ensembl T16G1.8
Entrez Gene 179777 Entrez gene
SwissProt Q9XUP5 EMBL-EBI
WormBase CE20078 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04730 Hypothetical protein T16G1.8 [Caenorhabditis elegans] ref|NP_506238.1| GRound-Like (grd related) family member (grl-2) [Caenorhabditis elegans] 1.0E-119
blast CAE64712 Hypothetical protein CBG09498 [Caenorhabditis briggsae] 9.0E-93
blast AAA81484 Ground-like (grd related) protein 4 [Caenorhabditis elegans] ref|NP_501166.2| GRound-Like (grd related) family member (grl-4) [Caenorhabditis elegans] 4.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 1.0E-30
Sequence
Target Sequence 
>C. ELEGANS:178663_AT
ctattgcaacaactccatcgtatttgcaaagtggttatgctccagctacactagcatatg
aaaagcagtatgatgaagagagtagtctggaaggaatggcaccacctccaccccctcctc
cgccaattgatataccaaaagatgtcaaaacctacgactaccgcacttcagaagttgtaa
gccattcggaaaactataaaccagcgtttgttccaagtgcaagttatttggaagatgtgt
cagaagaaggccaattagttgaaaaacaagggcattggtctaagggatcagattcccaga
cttcttcaaaatatggtcaatttgaaagtcgacataatattctgaaacgaatgaagacgg
agtcaataccaagaaccaataacacttgtaattcaataaaattagcaaatgtgatgatga
gagctattgtggatgatgtttctgtatcaaagagaatgattcaacatgccacaaagtcag
cgttcgatggtgccaaatttgatgtattttgtgcaattggagagttcagttatagtattc
actcccgcaagta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATTGCAACAACTCCATCGTATTT 389 223 512 Antisense
GCAAAGTGGTTATGCTCCAGCTACA 150 319 537 Antisense
CTCCTCCGCCAATTGATATACCAAA 21 235 626 Antisense
GATGTCAAAACCTACGACTACCGCA 494 405 652 Antisense
TACGACTACCGCACTTCAGAAGTTG 637 645 664 Antisense
CAAGGGCATTGGTCTAAGGGATCAG 108 183 778 Antisense
ATCAGATTCCCAGACTTCTTCAAAA 600 27 798 Antisense
GACGGAGTCAATACCAAGAACCAAT 564 375 867 Antisense
ACATGCCACAAAGTCAGCGTTCGAT 425 107 975 Antisense
AGTCAGCGTTCGATGGTGCCAAATT 394 59 986 Antisense
GTTATAGTATTCACTCCCGCAAGTA 362 447 1040 Antisense
Sequence Source Affymetrix Proprietary Database