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[178654_s_at]

Description W04A8.4 /REP_DB=WormBase Gene ID /WP=CE16538 /TR=Q9XUL8 /GB=CAB04908.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 38      4grp_highest_8_8 > 05      4grp_high_8_8 > 29      6grp_highest_nonorm > 85      6grp_total > 37      4grp_total > 34      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 173.4
(-3)
P 		347.6
(2.4)
P 		214.5
(-1.7)
P 		366.5
(3)
P 		193
B 179.6
(-3)
P 		392.8
(3)
P 		182.9
(-2.9)
P 		348.3
(1.7)
P 		213
C 154.5
(-3)
P 		402.6
(2.7)
P 		204.2
(-1.9)
P 		414.6
(3)
P 		260
avr 169.2
(-3) 		381
(3) 		200.5
(-2.1) 		376.5
(2.9) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 789
1 2 3 4 5 6 range
A 1041.4
(3)
P 		689.6
(-1)
P 		517.4
(-3)
P 		683.4
(-1.1)
P 		533.1
(-2.8)
P 		937
(1.8)
P 		524
B 1262.2
(3)
P 		654.3
(-1.8)
P 		558.7
(-2.5)
P 		496.7
(-3)
P 		510.6
(-2.9)
P 		906.6
(0.2)
P 		766
C 975.6
(3)
P 		698.7
(-0.3)
P 		473.7
(-3)
P 		741.6
(0.2)
P 		585.5
(-1.7)
P 		919.8
(2.3)
P 		502
avr 1093.1
(3) 		680.9
(-1.3) 		516.6
(-3) 		640.6
(-1.7) 		543.1
(-2.7) 		921.1
(1.2) 		577
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 63.1
(-0.4)
P 		75.4
(3)
P 		56.1
(-2.3)
P 		53.7
(-3)
P 		22
B 57.3
(-1.4)
P 		97
(3)
P 		42.4
(-3)
P 		74.2
(0.5)
P 		55
C 51.1
(-3)
P 		115.8
(3)
P 		71.2
(-1.1)
P 		69.9
(-1.3)
P 		65
avr 57.2
(-2.9) 		96.1
(3) 		56.6
(-3) 		65.9
(-1.6) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 327
1 2 3 4 5 6 range
A 192
(0.6)
P 		245.2
(3)
P 		112.7
(-3)
P 		173.3
(-0.3)
P 		160.9
(-0.8)
P 		228.1
(2.2)
P 		133
B 439.2
(3)
P 		217.8
(-1.3)
P 		135.7
(-2.9)
P 		131.6
(-3)
P 		207.5
(-1.5)
P 		338.1
(1)
P 		308
C 361.6
(3)
P 		213.2
(-0.7)
P 		120.6
(-3)
P 		149.1
(-2.3)
P 		189.5
(-1.3)
P 		307.9
(1.7)
P 		241
avr 330.9
(3) 		225.4
(-0) 		123
(-3) 		151.3
(-2.2) 		186
(-1.2) 		291.4
(1.9) 		208
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178654_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1115
Sequence Type Exemplar sequence
Representative Public ID W04A8.4 NCBI
Target Description W04A8.4 /REP_DB=WormBase Gene ID /WP=CE16538 /TR=Q9XUL8 /GB=CAB04908.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061021(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061021 NCBI Caenorhabditis elegans W04A8.4 (W04A8.4) mRNA, complete cds. 11/11 None
SNAP00000006526 ENSEMBL cdna:SNAP chromosome:CEL140:I:13849278:13850679:-1 9/11 None
GENEFINDER00000006538 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13850045:13854122:-1 10/11 None
W04A8.4 ENSEMBL cdna:known chromosome:CEL140:I:13850013:13851364:-1 gene:W04A8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13850183-13850673(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W04A8.4
Entrez Gene 173253 Entrez gene
SwissProt Q9XUL8 EMBL-EBI
WormBase CE16538 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04904 Hypothetical protein W04A8.3 [Caenorhabditis elegans] ref|NP_493423.1| W04A8.3 [Caenorhabditis elegans] 1.0E-159
blast CAB04908 Hypothetical protein W04A8.4 [Caenorhabditis elegans] ref|NP_493422.1| W04A8.4 [Caenorhabditis elegans] 7.0E-71
blast CAB04908 Hypothetical protein W04A8.4 [Caenorhabditis elegans] ref|NP_493422.1| W04A8.4 [Caenorhabditis elegans] 3.0E-56
blast CAB04908 Hypothetical protein W04A8.4 [Caenorhabditis elegans] ref|NP_493422.1| W04A8.4 [Caenorhabditis elegans] 4.0E-56
blast CAB60306 Hypothetical protein Y105C5A.14 [Caenorhabditis elegans] ref|NP_502882.1| Y105C5A.14 [Caenorhabditis elegans] 2.0E-25
blast CAA21514 Hypothetical protein Y43F8B.7 [Caenorhabditis elegans] ref|NP_507791.1| Y43F8B.7 [Caenorhabditis elegans] 1.0E-23
blast CAB04905 Hypothetical protein W04A8.5 [Caenorhabditis elegans] ref|NP_493424.1| W04A8.5 [Caenorhabditis elegans] 7.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR002562 EMBL-EBI 3-5 exonuclease 1.2E-5
Pfam IPR002562 EMBL-EBI 3-5 exonuclease 1.2E-5
Pfam IPR002562 EMBL-EBI 3-5 exonuclease 1.2E-5
Sequence
Target Sequence 
>C. ELEGANS:178654_S_AT
aagagcgcatctgtcaaaaaactaaaaaactttctgagaaaattatcatatcagagagac
atggtctcctatggtaaagaagattttttggcggacatcgagcacaaagacatgcaatca
aaattcgttcgcacgaacggaacgagaagtctcctcagtctaaaagctgaagcaacgtat
tggacaggattggacctccacaagggcgagactatgtccgattggacgaacactcatcta
cgtggagaccaactgagatatgctatcatggacacaatcgttatgcatctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGCGCATCTGTCAAAAAACTAA 299 151 16 Antisense
GAGACATGGTCTCCTATGGTAAAGA 230 393 71 Antisense
GATTTTTTGGCGGACATCGAGCACA 602 427 97 Antisense
AACGAGAAGTCTCCTCAGTCTAAAA 379 121 156 Antisense
GTATTGGACAGGATTGGACCTCCAC 644 451 192 Antisense
ATTGGACCTCCACAAGGGCGAGACT 603 1 204 Antisense
GGGCGAGACTATGTCCGATTGGACG 243 493 219 Antisense
GTCCGATTGGACGAACACTCATCTA 207 477 231 Antisense
GACGAACACTCATCTACGTGGAGAC 451 379 240 Antisense
GAGATATGCTATCATGGACACAATC 392 397 270 Antisense
TGGACACAATCGTTATGCATCTCAT 421 547 284 Antisense
Sequence Source Affymetrix Proprietary Database