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[178643_s_at]

Description C24F3.4 /REP_DB=WormBase Gene ID /WP=CE18521 /TR=Q9XXK6 /GB=CAA18773.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 33      4grp_high_8_8 > 51      6grp_highest_nonorm > 74      6grp_total > 65      4grp_total > 60      6grp_highest_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 386
1 2 3 4 range
A 662.5
(3)
P 		664
(3)
P 		621.4
(2.3)
P 		313.4
(-3)
P 		351
B 643.7
(2.3)
P 		631.2
(2)
P 		684.7
(3)
P 		347.8
(-3)
P 		337
C 681.5
(3)
P 		571.8
(1.3)
P 		615.2
(2)
P 		299.1
(-3)
P 		382
avr 662.6
(3) 		622.3
(2.3) 		640.4
(2.6) 		320.1
(-3) 		343

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 634.7
(-0.9)
P 		606.9
(-1.6)
P 		546.4
(-3)
P 		633.9
(-0.9)
P 		594.9
(-1.8)
P 		799.3
(3)
P 		253
B 600.1
(-2.9)
P 		595.9
(-3)
P 		804.6
(3)
P 		632.1
(-2)
P 		609.2
(-2.6)
P 		739.6
(1.1)
P 		209
C 521.1
(-3)
P 		604.4
(-0.8)
P 		682.4
(1.2)
P 		614.1
(-0.6)
P 		602.2
(-0.9)
P 		753.4
(3)
P 		232
avr 585.3
(-3) 		602.4
(-2.4) 		677.8
(0.1) 		626.7
(-1.6) 		602.1
(-2.4) 		764.1
(3) 		179
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 241.2
(3)
P 		144.1
(0)
P 		162.5
(0.6)
P 		45.9
(-3)
P 		195
B 205.3
(3)
P 		155.9
(0.7)
P 		158.7
(0.9)
P 		74.1
(-3)
P 		131
C 225.6
(3)
P 		164.4
(0.9)
P 		214.4
(2.6)
P 		50.4
(-3)
P 		175
avr 224
(3) 		154.8
(0.5) 		178.5
(1.4) 		56.8
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 117
(-3)
P 		215.8
(3)
P 		119
(-2.9)
P 		160.8
(-0.3)
P 		179.5
(0.8)
P 		194.6
(1.7)
P 		99
B 208.8
(-0.7)
P 		198.3
(-1.3)
P 		195.4
(-1.5)
P 		167.4
(-3)
P 		247.5
(1.4)
P 		275.8
(3)
P 		108
C 193.1
(0.2)
P 		184.4
(-0.2)
P 		173.7
(-0.7)
P 		123.5
(-3)
P 		194.9
(0.3)
P 		252.2
(3)
P 		129
avr 173
(-1.5) 		199.5
(0.2) 		162.7
(-2.2) 		150.6
(-3) 		207.3
(0.8) 		240.9
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178643_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8430
Sequence Type Exemplar sequence
Representative Public ID C24F3.4 NCBI
Target Description C24F3.4 /REP_DB=WormBase Gene ID /WP=CE18521 /TR=Q9XXK6 /GB=CAA18773.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069465(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069465 NCBI Caenorhabditis elegans C24F3.4 (C24F3.4) mRNA, complete cds. 11/11 None
SNAP00000043525 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10215046:10219276:-1 11/11 None
GENEFINDER00000043531 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10211755:10216111:-1 11/11 None
C24F3.4 ENSEMBL cdna:known chromosome:CEL140:IV:10214957:10219279:-1 gene:C24F3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10215051-10219282(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C24F3.4
Entrez Gene 177900 Entrez gene
SwissProt Q9XXK6 EMBL-EBI
WormBase CE18521 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6807 nitrogen compound metabolism inferred from electronic annotation QuickGO AmiGO
9435 NAD biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3952 NAD+ synthase (glutamine-hydrolyzing) activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA18773 Hypothetical protein C24F3.4 [Caenorhabditis elegans] ref|NP_501866.1| C24F3.4 [Caenorhabditis elegans] 0.0
blast CAE59908 Hypothetical protein CBG03392 [Caenorhabditis briggsae] 0.0
blast AAI03389 MGC127343 protein [Bos taurus] ref|NP_001029615.1| hypothetical protein LOC513400 [Bos taurus] 1.0E-177
blast AAI03389 MGC127343 protein [Bos taurus] ref|NP_001029615.1| hypothetical protein LOC513400 [Bos taurus] 1.0E-169
blast CAA18773 Hypothetical protein C24F3.4 [Caenorhabditis elegans] ref|NP_501866.1| C24F3.4 [Caenorhabditis elegans] 1.0E-154
Protein Domains
Database ID Description E-Value
Pfam IPR003010 EMBL-EBI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 3.1E-17
Pfam IPR003010 EMBL-EBI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 4.4E-7
Pfam IPR003694 EMBL-EBI NAD+ synthase 2.3E-50
Pfam IPR003694 EMBL-EBI NAD+ synthase 2.3E-50
Pfam IPR003694 EMBL-EBI NAD+ synthase 2.3E-50
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000043531 4 507-529,551-573,580-602,612-632
Sequence
Target Sequence 
>C. ELEGANS:178643_S_AT
ctcaactggctttggtctcacataaacgtcctggtggtcttcttgttttgggaacagcca
atgttgatgaaagtttggttggatatctgacaaaatatgattgctcatcagctgatatca
acccgattggctccgtgagcaaacgagatctgcgacaatttctggagatcgcgtacgaaa
agtatggaatggctgcgcttcgatgtgtgattgattcgacgccgaccgctgaactgaagc
cgttggttgatggaaaagttgcccagacagatgaagctgaaattggtttgacttatgatg
aattgtctgtgattggaagacttcgtaaacctggaggaatgggaccgtatggaatgtttt
tgaagttgctccagctctggggagataaatactcaattgacgagattgaagaaaaagtca
ataaattcttttggcggtaccgtgtcaatcgtcataaagcaactgtctcaactcctgcta
ttcatgctgaaaattacagtccggacgatcatcgaaacgaccatagaccattcctcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCAACTGGCTTTGGTCTCACATAA 339 227 1517 Antisense
ATAAACGTCCTGGTGGTCTTCTTGT 336 25 1538 Antisense
AGCTGATATCAACCCGATTGGCTCC 222 79 1626 Antisense
GGCTCCGTGAGCAAACGAGATCTGC 85 539 1645 Antisense
TGGCTGCGCTTCGATGTGTGATTGA 288 543 1706 Antisense
CGACCGCTGAACTGAAGCCGTTGGT 467 249 1739 Antisense
ATGTTTTTGAAGTTGCTCCAGCTCT 20 49 1870 Antisense
GCGGTACCGTGTCAATCGTCATAAA 632 291 1950 Antisense
TAAAGCAACTGTCTCAACTCCTGCT 347 639 1971 Antisense
TCAACTCCTGCTATTCATGCTGAAA 385 631 1984 Antisense
GAAACGACCATAGACCATTCCTCTA 221 355 2030 Antisense
Sequence Source Affymetrix Proprietary Database