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[178634_at]

Description R10E8.4 /REP_DB=WormBase Gene ID /WP=CE18912 /TR=Q9XUR9 /GB=CAB04641.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 33      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 53.8
(0.7)
A 		39
(-3)
A 		42.2
(-2.2)
A 		62.7
(3)
A 		24
B 41.7
(3)
A 		27.3
(-3)
A 		41.6
(3)
A 		40.9
(2.7)
A 		14
C 6.8
(-3)
A 		67.1
(3)
A 		45.4
(0.8)
A 		35.3
(-0.2)
A 		60
avr 34.1
(-3) 		44.5
(2.1) 		43.1
(1.4) 		46.3
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 13.8
(-2.1)
A 		43
(1.3)
A 		50.8
(2.2)
A 		39.4
(0.9)
A 		57.7
(3)
A 		6.2
(-3)
A 		52
B 29
(0.4)
A 		11.8
(-2.1)
A 		9.9
(-2.4)
A 		5.9
(-3)
A 		47.1
(3)
A 		26.3
(-0)
A 		41
C 33.2
(0.3)
A 		7.6
(-2.7)
A 		5.3
(-3)
A 		56.6
(3)
A 		17.2
(-1.6)
A 		36
(0.6)
A 		51
avr 25.3
(-1.6) 		20.8
(-3) 		22
(-2.6) 		34
(1) 		40.7
(3) 		22.8
(-2.4) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 19.6
(3)
A 		8.5
(-3)
A 		11
(-1.6)
A 		9.2
(-2.6)
A 		11
B 13.3
(3)
A 		6.7
(-3)
A 		9.6
(-0.4)
A 		8.7
(-1.2)
A 		7
C 2.3
(-3)
A 		19.3
(3)
A 		15.8
(1.8)
A 		6
(-1.7)
A 		17
avr 11.7
(2.4) 		11.5
(2.1) 		12.1
(3) 		8
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 2.5
(-2.6)
A 		15.3
(2.2)
A 		11.1
(0.6)
A 		10
(0.2)
A 		17.4
(3)
A 		1.5
(-3)
A 		16
B 10.1
(-0.1)
A 		3.9
(-2.2)
A 		2.4
(-2.7)
A 		1.6
(-3)
A 		19.1
(3)
A 		9.8
(-0.2)
A 		18
C 12.3
(3)
A 		2.3
(-2.5)
A 		1.3
(-3)
A 		11.4
(2.5)
A 		5.6
(-0.7)
A 		12
(2.8)
A 		11
avr 8.3
(-0.8) 		7.2
(-1.5) 		4.9
(-3) 		7.7
(-1.2) 		14
(3) 		7.8
(-1.1) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178634_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14625
Sequence Type Exemplar sequence
Representative Public ID R10E8.4 NCBI
Target Description R10E8.4 /REP_DB=WormBase Gene ID /WP=CE18912 /TR=Q9XUR9 /GB=CAB04641.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075168(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075168 NCBI Caenorhabditis elegans R10E8.4 (R10E8.4) mRNA, complete cds. 11/11 None
SNAP00000042679 ENSEMBL cdna:SNAP chromosome:CEL140:V:18249382:18252037:1 11/11 None
GENEFINDER00000042685 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18249382:18252037:1 11/11 None
R10E8.4 ENSEMBL cdna:known chromosome:CEL140:V:18249382:18252037:1 gene:R10E8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18249381-18252037(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10E8.4
Entrez Gene 187775 Entrez gene
SwissProt Q9XUR9 EMBL-EBI
WormBase CE18912 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04641 Hypothetical protein R10E8.4 [Caenorhabditis elegans] ref|NP_507569.1| R10E8.4 [Caenorhabditis elegans] 0.0
blast CAB04644 Hypothetical protein R10E8.3 [Caenorhabditis elegans] ref|NP_507567.1| R10E8.3 [Caenorhabditis elegans] 7.0E-82
blast CAB03320 Hypothetical protein T06E6.5 [Caenorhabditis elegans] ref|NP_506829.1| T06E6.5 [Caenorhabditis elegans] 8.0E-14
Sequence
Target Sequence 
>C. ELEGANS:178634_AT
gacaccgattctgcgattgttcgtatttccggcgatggcgacgaagtcactgggaactgc
tccgagatttcatcaagctggctggaaaagtatggcgaaaatattcaatcgttttcttgt
gattttcacggttattcttcgcaaaagatgcaagagctgaagaacttcctacactcttcc
aagaacaagatttccatggccagcttgaagctgaaagggaatagtcactggaagcacgct
gtggacttggttttaacaggagccaatattttggtttccatccaacgtcttcaacacttt
cttagagtgaaggctttcttggagactattccagttgaagatgtgatcttcttgaagaag
gcacttcttccttcttcacttcccagaaacttcgagctcactacttccgacaatttcgat
ttgcaaagcctttcgattggatgtccagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACCGATTCTGCGATTGTTCGTA 373 367 1036 Antisense
TGTTCGTATTTCCGGCGATGGCGAC 696 561 1053 Antisense
AAGTCACTGGGAACTGCTCCGAGAT 710 155 1079 Antisense
GATTTTCACGGTTATTCTTCGCAAA 328 427 1156 Antisense
TGAAGAACTTCCTACACTCTTCCAA 294 575 1193 Antisense
AGATTTCCATGGCCAGCTTGAAGCT 557 65 1223 Antisense
AGCACGCTGTGGACTTGGTTTTAAC 27 77 1268 Antisense
ATCCAACGTCTTCAACACTTTCTTA 97 41 1315 Antisense
CTTCCCAGAAACTTCGAGCTCACTA 308 219 1414 Antisense
AGCTCACTACTTCCGACAATTTCGA 399 75 1430 Antisense
AAAGCCTTTCGATTGGATGTCCAGC 130 125 1460 Antisense
Sequence Source Affymetrix Proprietary Database