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[178628_s_at]

Description T23G7.4 /REP_DB=WormBase Gene ID /WP=CE03705 /TR=Q22706 /GB=CAA92702.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 72      6grp_total > 54      4grp_total > 55      6grp_cgc > 82      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 215
1 2 3 4 range
A 485.9
(3)
P 		298.2
(-3)
P 		336.1
(-1.8)
P 		307.3
(-2.7)
P 		188
B 485.8
(3)
P 		283.3
(-3)
P 		350.6
(-1)
P 		296.1
(-2.6)
P 		203
C 498.3
(3)
P 		290.9
(-3)
P 		358
(-1.1)
P 		319.8
(-2.2)
P 		207
avr 490
(3) 		290.8
(-3) 		348.2
(-1.3) 		307.7
(-2.5) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 270
1 2 3 4 5 6 range
A 412.3
(-1.3)
P 		478.3
(1.2)
P 		526.7
(3)
P 		406.4
(-1.5)
P 		367.2
(-3)
P 		386.3
(-2.3)
P 		160
B 426.3
(-1.2)
P 		450.1
(-0.5)
P 		556.7
(3)
P 		471.1
(0.2)
P 		371.6
(-3)
P 		471.8
(0.2)
P 		185
C 371.2
(-0.6)
P 		494.1
(3)
P 		459.1
(2)
P 		494.9
(3)
P 		287
(-3)
P 		477.7
(2.5)
P 		208
avr 403.3
(-0.9) 		474.2
(1.6) 		514.2
(3) 		457.5
(1) 		341.9
(-3) 		445.3
(0.6) 		172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 176.9
(3)
P 		64.7
(-2.1)
P 		87.9
(-1)
P 		45
(-3)
P 		132
B 154.9
(3)
P 		70
(-2.5)
P 		81.2
(-1.8)
P 		63.1
(-3)
P 		92
C 164.9
(3)
P 		83.6
(-1.4)
P 		124.8
(0.8)
P 		53.9
(-3)
P 		111
avr 165.6
(3) 		72.8
(-2) 		98
(-0.6) 		54
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 76
(-3)
P 		170.1
(3)
P 		114.7
(-0.5)
P 		103.1
(-1.3)
P 		110.8
(-0.8)
P 		94.1
(-1.8)
P 		94
B 148.3
(-0.2)
P 		149.8
(-0.1)
P 		135.2
(-1.8)
P 		124.8
(-3)
P 		151
(0.1)
P 		175.9
(3)
P 		51
C 137.6
(1)
P 		150.8
(2.2)
P 		116.8
(-0.9)
P 		99.5
(-2.4)
P 		92.9
(-3)
P 		159.9
(3)
P 		67
avr 120.6
(-1.6) 		156.9
(3) 		122.2
(-1.4) 		109.1
(-3) 		118.2
(-1.9) 		143.3
(1.3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178628_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3148
Sequence Type Exemplar sequence
Representative Public ID T23G7.4 NCBI
Target Description T23G7.4 /REP_DB=WormBase Gene ID /WP=CE03705 /TR=Q22706 /GB=CAA92702.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063557(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063557 NCBI Caenorhabditis elegans yeast SEC homolog family member (sec-5) (sec-5) mRNA, complete cds. 11/11 None
SNAP00000032786 ENSEMBL cdna:SNAP chromosome:CEL140:II:9173232:9177057:-1 11/11 None
GENEFINDER00000032794 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9173232:9177057:-1 11/11 None
T23G7.4 ENSEMBL cdna:known chromosome:CEL140:II:9173085:9177062:-1 gene:T23G7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9173228-9177054(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sec-5
Ensembl T23G7.4
Entrez Gene 174459 Entrez gene
SwissProt Q22706 EMBL-EBI
WormBase CE03705 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.14491.1.S1_AT similar to Sec5 protein cfa
CANINE_2:CFAAFFX.14493.1.S1_S_AT similar to Sec5 protein cfa
DROSOPHILA_2:1629189_AT dm
DROSGENOME1:141559_AT dm
CHICKEN:GGAAFFX.8129.1.S1_AT similar to SEC5 homolog; SEC5 homolog (S. cerevisiae) gga
HG-U133_PLUS_2:226270_AT SEC5-like 1 (S. cerevisiae) hs
HG-U133B:226270_AT SEC5-like 1 (S. cerevisiae) hs
HG-U133_PLUS_2:219349_S_AT SEC5-like 1 (S. cerevisiae) hs
HG-U133A:219349_S_AT SEC5-like 1 (S. cerevisiae) hs
HG-U133A_2:219349_S_AT SEC5-like 1 (S. cerevisiae) hs
HU35KSUBA:RC_AA161357_AT SEC5-like 1 (S. cerevisiae) hs
HU35KSUBD:RC_AA342622_AT SEC5-like 1 (S. cerevisiae) hs
U133_X3P:G8922829_3P_A_AT SEC5-like 1 (S. cerevisiae) hs
U133_X3P:HS.16580.1.S1_3P_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95C:57344_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95C:57812_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95B:58333_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95B:42758_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95C:50790_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95C:60661_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95E:67701_AT SEC5-like 1 (S. cerevisiae) hs
HG-U95E:69797_R_AT SEC5-like 1 (S. cerevisiae) hs
HU35KSUBA:AA136369_AT SEC5-like 1 (S. cerevisiae) hs
MG-U74BV2:114038_AT SEC5-like 1 (S. cerevisiae) mm
MG-U74BV2:163876_AT SEC5-like 1 (S. cerevisiae) mm
MOE430B:1428470_AT SEC5-like 1 (S. cerevisiae) mm
MOUSE430_2:1428470_AT SEC5-like 1 (S. cerevisiae) mm
MU19KSUBB:TC32481_AT SEC5-like 1 (S. cerevisiae) (Sec5l1), mRNA mm
RG-U34A:AF032666_AT Rsec5 protein rn
RG-U34A:AF032666_G_AT Rsec5 protein rn
RAE230A:1368225_AT Rsec5 protein rn
RAT230_2:1368225_AT Rsec5 protein rn
RG-U34C:RC_AI101112_AT Rsec5 protein rn
Protein Similarities
Method ID Description E-Value
blast CAA92702 Hypothetical protein T23G7.4 [Caenorhabditis elegans] ref|NP_495958.1| yeast SEC homolog family member (sec-5) [Caenorhabditis elegans] sp|Q22706|SEC5_CAEEL Probable exocyst complex component Sec5 0.0
blast CAE57882 Hypothetical protein CBG00928 [Caenorhabditis briggsae] 0.0
blast XP_535863 PREDICTED: similar to Sec5 protein [Canis familiaris] 5.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 8.8E-14
Sequence
Target Sequence 
>C. ELEGANS:178628_S_AT
aatctcgagcaaaactttccgcattccgaactaaaatgatcaatgattgcattgcgatga
tgagcagtgcatttgaacctcttattgcttcagcaacttatgagtacttaccagatgatg
atgatatctccgattacgccaaggaaatgatcctctgctctgtgctccaacaagcagaac
tagaactttatgctccacagctagcaattgaatgtctccaacaaacagtatcaaatgctc
ttgatgctcttcttcttcatttttcccgactgaatcaagatacatcaccattgattgtaa
cacaaattgtcattgatttgactggtcttgaagaggctctctcaacgtatgccacgctgg
caattcgggtgcaaataaatacttatagagcaggactcgtcggacgatttgataatcaac
gtcttcagcaatgccttaaaaacatgcgaaccactatgcgaatggctcttcaaagtctag
agcaacacgccgagaaccttggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCTCGAGCAAAACTTTCCGCATT 587 169 2135 Antisense
AGCAGTGCATTTGAACCTCTTATTG 103 79 2197 Antisense
GATGATGATATCTCCGATTACGCCA 320 411 2251 Antisense
AAGGAAATGATCCTCTGCTCTGTGC 346 161 2275 Antisense
GAACTAGAACTTTATGCTCCACAGC 36 351 2311 Antisense
CTTCTTCATTTTTCCCGACTGAATC 90 219 2386 Antisense
AAGAGGCTCTCTCAACGTATGCCAC 192 155 2465 Antisense
ATGCCACGCTGGCAATTCGGGTGCA 196 41 2483 Antisense
ATAGAGCAGGACTCGTCGGACGATT 506 19 2519 Antisense
GAACCACTATGCGAATGGCTCTTCA 63 345 2582 Antisense
AGAGCAACACGCCGAGAACCTTGGC 375 69 2613 Antisense
Sequence Source Affymetrix Proprietary Database