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[178616_at]

Description T21B4.3 /REP_DB=WormBase Gene ID /WP=CE16428 /TR=O18096 /GB=CAB03368.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 09      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 2.6
(-3)
A 		2.6
(-3)
A 		6.8
(1.5)
A 		8.2
(3)
A 		6
B 1.4
(-3)
A 		3.4
(-1.6)
A 		4.9
(-0.5)
A 		9.7
(3)
A 		8
C 1.7
(-3)
A 		6.5
(-1.7)
A 		2.6
(-2.8)
A 		24.6
(3)
A 		23
avr 1.9
(-3) 		4.2
(-1.9) 		4.8
(-1.6) 		14.2
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 3.9
(-1.5)
A 		2.3
(-2.5)
A 		1.4
(-3)
A 		11.3
(3)
A 		1.8
(-2.8)
A 		2.2
(-2.5)
A 		10
B 1.2
(-3)
A 		7.5
(3)
A 		2.6
(-1.7)
A 		1.3
(-2.9)
A 		2.2
(-2)
A 		3.7
(-0.6)
A 		6
C 2.3
(-1.9)
A 		7.8
(3)
A 		5.3
(0.8)
A 		4.2
(-0.2)
A 		2.1
(-2.1)
A 		1.1
(-3)
A 		7
avr 2.5
(-2.2) 		5.9
(3) 		3.1
(-1.3) 		5.6
(2.5) 		2
(-3) 		2.3
(-2.5) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 0.9
(-1.5)
A 		0.6
(-3)
A 		1.8
(3)
A 		1.2
(-0)
A 		1
B 0.4
(-3)
A 		0.9
(-1.2)
A 		1.1
(-0.5)
A 		2.1
(3)
A 		2
C 0.5
(-3)
A 		1.9
(-0.7)
A 		0.9
(-2.3)
A 		4.1
(3)
A 		4
avr 0.6
(-3) 		1.1
(-1.4) 		1.3
(-0.8) 		2.5
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 0.7
(-2.1)
A 		0.8
(-1.8)
A 		0.3
(-3)
A 		2.9
(3)
A 		0.5
(-2.5)
A 		0.5
(-2.5)
A 		3
B 0.4
(-2.7)
A 		2.5
(3)
A 		0.6
(-2.2)
A 		0.3
(-3)
A 		0.9
(-1.4)
A 		1.4
(-0)
A 		2
C 0.8
(-1.8)
A 		2.4
(3)
A 		1.3
(-0.3)
A 		0.8
(-1.8)
A 		0.7
(-2.1)
A 		0.4
(-3)
A 		2
avr 0.6
(-3) 		1.9
(3) 		0.7
(-2.5) 		1.3
(0.2) 		0.7
(-2.5) 		0.8
(-2.1) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178616_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3772
Sequence Type Exemplar sequence
Representative Public ID T21B4.3 NCBI
Target Description T21B4.3 /REP_DB=WormBase Gene ID /WP=CE16428 /TR=O18096 /GB=CAB03368.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064265(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064265 NCBI Caenorhabditis elegans T21B4.3 (T21B4.3) mRNA, complete cds. 11/11 None
SNAP00000006685 ENSEMBL cdna:SNAP chromosome:CEL140:II:12495480:12496290:-1 11/11 None
GENEFINDER00000006697 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12495480:12496290:-1 11/11 None
T21B4.3 ENSEMBL cdna:known chromosome:CEL140:II:12495480:12496290:-1 gene:T21B4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12495476-12496287(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21B4.3
Entrez Gene 188673 Entrez gene
SwissProt O18096 EMBL-EBI
WormBase CE16428 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03368 Hypothetical protein T21B4.3 [Caenorhabditis elegans] ref|NP_496666.1| T21B4.3 [Caenorhabditis elegans] 2.0E-55
blast CAE58724 Hypothetical protein CBG01909 [Caenorhabditis briggsae] 8.0E-19
blast CAA88543 Hypothetical protein R07B1.6 [Caenorhabditis elegans] ref|NP_509654.1| R07B1.6 [Caenorhabditis elegans] sp|Q09420|YRN6_CAEEL Hypothetical protein R07B1.6 in chromosome X 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR002601 EMBL-EBI Protein of unknown function C6 2.4E-29
Sequence
Target Sequence 
>C. ELEGANS:178616_AT
gctatactatcctggcttactacttcaggaaattcatgtattgcaacaagcccgacaaca
actgtaactaccacagacgcctccgtaaccactacaccacccacgactacaccacccacg
actgcagtcgtgacagattgcaccacgtgtacggctgcgctgattacgttaacccaaggc
actgatggagcggtggatgccactccggtagaaggagtcgatgcaactggctgtgcaact
attacctatacttgtgaaaatactccggtagatgcacagaatgtcatttttcttaccttc
tacggtggcggtatagatcgaggcgttgatcaaggcaccggatccctgaacgtgttgctg
aattgtgtcgacggagcttgggtcagaggcggtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTATACTATCCTGGCTTACTACTT 49 301 40 Antisense
GTATTGCAACAAGCCCGACAACAAC 81 453 77 Antisense
GTGACAGATTGCACCACGTGTACGG 398 481 169 Antisense
GTGTACGGCTGCGCTGATTACGTTA 191 485 186 Antisense
GATTACGTTAACCCAAGGCACTGAT 369 423 201 Antisense
GAAGGAGTCGATGCAACTGGCTGTG 268 333 250 Antisense
ACTGGCTGTGCAACTATTACCTATA 191 97 265 Antisense
GAATGTCATTTTTCTTACCTTCTAC 365 331 318 Antisense
TCGAGGCGTTGATCAAGGCACCGGA 42 601 357 Antisense
GAACGTGTTGCTGAATTGTGTCGAC 698 347 387 Antisense
GACGGAGCTTGGGTCAGAGGCGGTC 398 375 409 Antisense
Sequence Source Affymetrix Proprietary Database