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[178589_at]

Description T08G3.3 /REP_DB=WormBase Gene ID /WP=CE16373 /TR=O18071 /GB=CAB05794.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 0      4grp_total > 05      6grp_total_sub > Hat      6grp_cgc > 64      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 40.6
(-0.7)
A 		7.5
(-3)
A 		25.9
(-1.7)
A 		95.2
(3)
A 		88
B 39.9
(0.1)
A 		6.2
(-3)
A 		24.1
(-1.4)
A 		72.1
(3)
A 		66
C 56.5
(0.3)
A 		32.8
(-1.2)
A 		4.2
(-3)
A 		99.4
(3)
P 		95
avr 45.7
(-0.5) 		15.5
(-3) 		18.1
(-2.8) 		88.9
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 7.1
(-2.2)
A 		5.3
(-3)
A 		9
(-1.4)
A 		19.6
(3)
A 		11.9
(-0.2)
A 		5.6
(-2.9)
A 		14
B 14.9
(-0.6)
A 		11.7
(-1.2)
A 		7.9
(-1.9)
A 		10.1
(-1.5)
A 		33.2
(3)
A 		2.4
(-3)
A 		31
C 8.6
(-2.5)
A 		52
(3)
A 		11.5
(-2.2)
A 		11
(-2.2)
A 		4.9
(-3)
A 		29
(0.1)
A 		47
avr 10.2
(-2.7) 		23
(3) 		9.5
(-3) 		13.6
(-1.2) 		16.7
(0.2) 		12.3
(-1.8) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 14.8
(3)
A 		1.6
(-3)
A 		6.8
(-0.6)
A 		13.9
(2.6)
A 		13
B 12.7
(1.8)
A 		1.5
(-3)
A 		5.6
(-1.2)
A 		15.4
(3)
A 		14
C 18.7
(3)
A 		9.4
(-0.2)
A 		1.5
(-3)
A 		16.8
(2.3)
P 		17
avr 15.4
(3) 		4.2
(-3) 		4.6
(-2.8) 		15.4
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 1.3
(-3)
A 		1.9
(-2)
A 		2
(-1.9)
A 		5
(3)
A 		3.6
(0.7)
A 		1.4
(-2.8)
A 		4
B 5.2
(-1)
A 		3.9
(-1.6)
A 		1.9
(-2.5)
A 		2.7
(-2.1)
A 		13.5
(3)
A 		0.9
(-3)
A 		13
C 3.2
(-2.3)
A 		15.9
(3)
A 		2.9
(-2.5)
A 		2.2
(-2.7)
A 		1.6
(-3)
A 		9.7
(0.4)
A 		14
avr 3.2
(-1.9) 		7.2
(3) 		2.3
(-3) 		3.3
(-1.8) 		6.2
(1.8) 		4
(-0.9) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178589_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13394
Sequence Type Exemplar sequence
Representative Public ID T08G3.3 NCBI
Target Description T08G3.3 /REP_DB=WormBase Gene ID /WP=CE16373 /TR=O18071 /GB=CAB05794.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074762(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074762 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-142) (srh-142) mRNA, complete cds. 11/11 None
SNAP00000031134 ENSEMBL cdna:SNAP chromosome:CEL140:V:16461971:16462618:-1 11/11 None
T08G3.3 ENSEMBL cdna:known chromosome:CEL140:V:16461971:16463547:-1 gene:T08G3.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000031145 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16461970-16463547(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-142
Ensembl T08G3.3
Entrez Gene 188294 Entrez gene
SwissProt O18071 EMBL-EBI
WormBase CE16373 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05794 Hypothetical protein T08G3.3 [Caenorhabditis elegans] ref|NP_507163.1| Serpentine Receptor, class H family member (srh-142) [Caenorhabditis elegans] 1.0E-176
blast CAB05794 Hypothetical protein T08G3.3 [Caenorhabditis elegans] ref|NP_507163.1| Serpentine Receptor, class H family member (srh-142) [Caenorhabditis elegans] 1.0E-119
blast CAB04175 Hypothetical protein F26D2.4 [Caenorhabditis elegans] ref|NP_507147.1| Serpentine Receptor, class H family member (srh-145) [Caenorhabditis elegans] 1.0E-102
blast CAB05795 Hypothetical protein T08G3.5 [Caenorhabditis elegans] ref|NP_507159.1| Serpentine Receptor, class H family member (srh-141) [Caenorhabditis elegans] 1.0E-102
blast T21401 hypothetical protein F26D2.5 - Caenorhabditis elegans 8.0E-64
blast CAB04175 Hypothetical protein F26D2.4 [Caenorhabditis elegans] ref|NP_507147.1| Serpentine Receptor, class H family member (srh-145) [Caenorhabditis elegans] 2.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 7.1E-101
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507163.1 7 15-37,58-80,95-117,139-158,201-223,244-263,278-297
T08G3.3 7 15-37,58-80,95-117,139-158,201-223,244-263,278-297
SNAP00000031134 4 25-44,87-109,130-149,164-183
Sequence
Target Sequence 
>C. ELEGANS:178589_AT
ttatcagttatcactttcatcccaccagtaattgataatcctaacagagtagaagtcctg
gaagcttctcatcggaagttcccatgcctaccaccagagataattgataatccaagacta
ttcgtgatgggaactgataacaacacgtttatcgcttgtgtggtcccttatattataatt
ggatgggttcaaatacttggattctttgttggaactgttaactttatttatagaactaaa
actatgtctctacacacttcaaatctccaaaagaaattcttcaaatccctctgcattcaa
attgccgttccacttgtagtcctccttatcccagagtcttatattttgaatacggctatt
tctggaaatatggatgtaggattgaccaatattctaatgatttggatggctagtcacgga
ttgttctcaactgttgtcatgctcattgtgcacaaaccgtatcgaaaagcaactctaagt
attttaggtcgaccgcaggcaatcaaaacagctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATCAGTTATCACTTTCATCCCAC 182 681 454 Antisense
TCTCATCGGAAGTTCCCATGCCTAC 444 613 520 Antisense
CCATGCCTACCACCAGAGATAATTG 30 273 535 Antisense
ATCGCTTGTGTGGTCCCTTATATTA 349 37 604 Antisense
TGTCTCTACACACTTCAAATCTCCA 5 559 698 Antisense
AGAAATTCTTCAAATCCCTCTGCAT 30 73 725 Antisense
CCCTCTGCATTCAAATTGCCGTTCC 705 255 740 Antisense
TCCTCCTTATCCCAGAGTCTTATAT 321 593 773 Antisense
GGATGGCTAGTCACGGATTGTTCTC 574 513 857 Antisense
TCAACTGTTGTCATGCTCATTGTGC 433 631 880 Antisense
GACCGCAGGCAATCAAAACAGCTCC 181 383 944 Antisense
Sequence Source Affymetrix Proprietary Database