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[178574_at]

Description F43G9.4 /REP_DB=WormBase Gene ID /WP=CE10360 /TR=Q93718 /GB=CAB02108.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 87      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 83      6grp_total > 97      4grp_total > 44      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 405
(3)
P 		396.7
(2.4)
P 		332.9
(-1.9)
P 		317.6
(-3)
P 		87
B 336.1
(-2.6)
P 		434.7
(3)
P 		332.3
(-2.8)
P 		328.3
(-3)
P 		106
C 400.1
(3)
P 		376.6
(1.9)
P 		313.1
(-1)
P 		268.5
(-3)
P 		132
avr 380.4
(1.6) 		402.7
(3) 		326.1
(-1.7) 		304.8
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 408.5
(0.1)
P 		342
(-2.2)
P 		317.4
(-3)
P 		427.2
(0.8)
P 		423.8
(0.6)
P 		492.4
(3)
P 		175
B 433.5
(-0.6)
P 		377.7
(-3)
P 		437.1
(-0.5)
P 		402.2
(-2)
P 		479.3
(1.3)
P 		518
(3)
P 		140
C 414.2
(-1.8)
P 		350.6
(-3)
P 		418.5
(-1.7)
P 		478
(-0.6)
P 		490
(-0.3)
P 		662.6
(3)
P 		312
avr 418.7
(-1.2) 		356.8
(-3) 		391
(-2) 		435.8
(-0.6) 		464.4
(0.2) 		557.7
(3) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 147.5
(3)
P 		86.1
(-0.6)
P 		87
(-0.6)
P 		46.5
(-3)
P 		101
B 107.2
(3)
P 		107.4
(3)
P 		77
(-1.9)
P 		70
(-3)
P 		37
C 132.5
(3)
P 		108.3
(1.3)
P 		109.1
(1.4)
P 		45.3
(-3)
P 		87
avr 129.1
(3) 		100.6
(0.7) 		91
(-0) 		53.9
(-3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 75.3
(-2.4)
P 		121.6
(2.4)
P 		69.1
(-3)
P 		108.3
(1)
P 		127.9
(3)
P 		119.9
(2.2)
P 		59
B 150.8
(0)
P 		125.7
(-1.7)
P 		106.1
(-3)
P 		106.5
(-3)
P 		194.7
(3)
P 		193.2
(2.9)
P 		89
C 153.5
(-0.3)
P 		107
(-2.5)
P 		106.5
(-2.5)
P 		96.1
(-3)
P 		158.6
(-0)
P 		221.8
(3)
P 		126
avr 126.5
(-0.7) 		118.1
(-1.3) 		93.9
(-3) 		103.6
(-2.3) 		160.4
(1.7) 		178.3
(3) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178574_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2196
Sequence Type Exemplar sequence
Representative Public ID F43G9.4 NCBI
Target Description F43G9.4 /REP_DB=WormBase Gene ID /WP=CE10360 /TR=Q93718 /GB=CAB02108.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059931(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059931 NCBI Caenorhabditis elegans F43G9.4 (F43G9.4) mRNA, complete cds. 11/11 None
SNAP00000043537 ENSEMBL cdna:SNAP chromosome:CEL140:I:8619304:8622887:-1 11/11 None
GENEFINDER00000043548 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8619304:8620710:-1 11/11 None
F43G9.4 ENSEMBL cdna:known chromosome:CEL140:I:8619019:8620710:-1 gene:F43G9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8618949-8620641(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F43G9.4
Entrez Gene 259386 Entrez gene
SwissProt Q93718 EMBL-EBI
WormBase CE10360 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02108 Hypothetical protein F43G9.4 [Caenorhabditis elegans] ref|NP_492332.1| F43G9.4 [Caenorhabditis elegans] 0.0
blast CAB02108 Hypothetical protein F43G9.4 [Caenorhabditis elegans] ref|NP_492332.1| F43G9.4 [Caenorhabditis elegans] 1.0E-166
blast CAB02107 Hypothetical protein F43G9.3 [Caenorhabditis elegans] ref|NP_492333.1| F43G9.3 [Caenorhabditis elegans] 1.0E-151
blast CAE67090 Hypothetical protein CBG12501 [Caenorhabditis briggsae] 1.0E-143
blast CAB02108 Hypothetical protein F43G9.4 [Caenorhabditis elegans] ref|NP_492332.1| F43G9.4 [Caenorhabditis elegans] 1.0E-126
blast CAE67091 Hypothetical protein CBG12502 [Caenorhabditis briggsae] 3.0E-85
blast CAE67091 Hypothetical protein CBG12502 [Caenorhabditis briggsae] 4.0E-85
blast CAC70153 hypothetical protein [Brugia malayi] 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 9.7E-33
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 1.3E-29
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 6.4E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000043537 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:178574_AT
gatttaatgtcaccagtttccaagaaaccttcgtcttccgaacgctcaacaccatccgga
tcacaacaacaacctccaattaccgaagaacaatcgaaatctatgatgaaaatgattgat
gatcgttttgaagttctgatcaatgaaatctggaagagtgatgaagtggctcaatccaaa
aaccagactatgaaggataaaatgacgatcaaattgaaaaacaatcagtttctgaaagaa
tccgactacgacatagtcttcgagcgaatattcaattttatccgcatgcagaatccgtca
ctttccttcatttccacattcaatatcacatcttctcacatcattccacacatactgatc
acttgttattcagcgtgtagaaatttggattatcaaacttcaaaagaaattttctatcaa
ctaaatactcgcgtcttaggcttggattcatcaatgagcattcctgtcactgatcacctg
gaagagctttgccgcttggcatgtgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTAATGTCACCAGTTTCCAAGA 124 427 469 Antisense
ACCATCCGGATCACAACAACAACCT 251 85 519 Antisense
CAACAACAACCTCCAATTACCGAAG 702 187 532 Antisense
ATTTTATCCGCATGCAGAATCCGTC 330 15 743 Antisense
TTCCTTCATTTCCACATTCAATATC 666 699 771 Antisense
ATTCAATATCACATCTTCTCACATC 208 13 786 Antisense
TCTCACATCATTCCACACATACTGA 188 613 802 Antisense
AATACTCGCGTCTTAGGCTTGGATT 612 169 892 Antisense
CAATGAGCATTCCTGTCACTGATCA 178 183 920 Antisense
TGATCACCTGGAAGAGCTTTGCCGC 480 565 939 Antisense
GAGCTTTGCCGCTTGGCATGTGATG 694 387 952 Antisense
Sequence Source Affymetrix Proprietary Database