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[178557_at]

Description F14H8.4 /REP_DB=WormBase Gene ID /WP=CE09395 /TR=Q93484 /GB=CAB02933.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 15      4grp_total > 37      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 86.5
(3)
A 		75
(1.7)
A 		32.1
(-3)
A 		41.5
(-2)
A 		54
B 85.8
(2.2)
A 		96.8
(3)
A 		13.8
(-3)
A 		78.7
(1.7)
A 		83
C 132.2
(3)
A 		87.1
(-0.2)
A 		47.8
(-3)
A 		94.2
(0.3)
A 		84
avr 101.5
(3) 		86.3
(1.7) 		31.2
(-3) 		71.5
(0.4) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 95.2
(3)
A 		74.9
(1.7)
A 		81.9
(2.1)
A 		37.6
(-0.7)
A 		8.4
(-2.6)
A 		2.1
(-3)
A 		93
B 106.9
(3)
A 		73.5
(0.6)
M 		75.8
(0.7)
A 		25
(-3)
A 		67.9
(0.1)
A 		54.9
(-0.8)
A 		82
C 83.8
(1.3)
A 		106.6
(3)
A 		26.2
(-3)
A 		91.2
(1.9)
A 		52.5
(-1)
A 		39.1
(-2)
A 		80
avr 95.3
(3) 		85
(2) 		61.3
(-0.2) 		51.3
(-1.2) 		42.9
(-2) 		32
(-3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 31.5
(3)
A 		16.3
(-0.6)
A 		8.4
(-2.5)
A 		6.1
(-3)
A 		25
B 27.4
(3)
A 		23.9
(2.1)
A 		3.2
(-3)
A 		16.8
(0.4)
A 		24
C 43.8
(3)
A 		25
(-1)
A 		16.6
(-2.8)
A 		15.9
(-3)
A 		28
avr 34.2
(3) 		21.7
(-0) 		9.4
(-3) 		12.9
(-2.2) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 17.5
(0.9)
A 		26.6
(3)
A 		17.8
(1)
A 		9.5
(-0.9)
A 		2.5
(-2.5)
A 		0.5
(-3)
A 		26
B 37.2
(3)
A 		24.5
(0.5)
M 		18.4
(-0.7)
A 		6.6
(-3)
A 		27.6
(1.1)
A 		20.5
(-0.3)
A 		31
C 31.1
(2.7)
A 		32.5
(3)
A 		6.7
(-3)
A 		18.3
(-0.3)
A 		17
(-0.6)
A 		13.1
(-1.5)
A 		26
avr 28.6
(3) 		27.9
(2.8) 		14.3
(-2) 		11.5
(-3) 		15.7
(-1.5) 		11.4
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178557_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13217
Sequence Type Exemplar sequence
Representative Public ID F14H8.4 NCBI
Target Description F14H8.4 /REP_DB=WormBase Gene ID /WP=CE09395 /TR=Q93484 /GB=CAB02933.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074297(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074297 NCBI Caenorhabditis elegans F14H8.4 (F14H8.4) mRNA, complete cds. 11/11 None
SNAP00000006134 ENSEMBL cdna:SNAP chromosome:CEL140:V:15026450:15027574:1 11/11 None
GENEFINDER00000006140 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15026450:15027574:1 11/11 None
F14H8.4 ENSEMBL cdna:known chromosome:CEL140:V:15026450:15027574:1 gene:F14H8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15026449-15027574(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H8.4
Entrez Gene 184499 Entrez gene
SwissProt Q93484 EMBL-EBI
WormBase CE09395 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02933 Hypothetical protein F14H8.4 [Caenorhabditis elegans] ref|NP_506698.1| F14H8.4 [Caenorhabditis elegans] 1.0E-134
blast CAB02932 Hypothetical protein F14H8.5 [Caenorhabditis elegans] ref|NP_506697.1| F14H8.5 [Caenorhabditis elegans] 3.0E-51
Sequence
Target Sequence 
>C. ELEGANS:178557_AT
ctgtgtgtatcctactaatcgccgtaagacccctgaagaattggagatgtaccgaatgaa
cgcaaagagtttgtccgagtgtcaagccggagaatttccattttatggagatgaaggaac
tgccaagaactatagacattcgatcggaaatattctagccaaaatgacaactctcaaggc
gtactatgatcaggtttacactactggcttgcgaggaggcaagataaatggacttattca
gctattctgcaaccaagtcaacatcctctgtcatcactatgagatcttcttccgtgaatc
atctgttaaagttgacaaatacgtagagaagcgaaagcagattgcacgcgatggttacaa
ggatctcaagaatctcagtctaatcatttatctgaacgaaagaagaaatccaatactctt
caaacttgttcggcaacgtgttaaatttcaagctcggcaggttcaaaaactcgtagatca
cttccacagaaccttctgtccagttctgacaaatgcgattccaaagccgaatgctccacc
aatgagaattgcagcttctccgacaagtgctcagattattccgttttacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTGTGTATCCTACTAATCGCCGT 250 241 90 Antisense
AAGAGTTTGTCCGAGTGTCAAGCCG 242 155 154 Antisense
TACACTACTGGCTTGCGAGGAGGCA 105 643 286 Antisense
ATTCTGCAACCAAGTCAACATCCTC 188 11 333 Antisense
TCAACATCCTCTGTCATCACTATGA 119 631 347 Antisense
TACTCTTCAAACTTGTTCGGCAACG 621 645 503 Antisense
AAAACTCGTAGATCACTTCCACAGA 579 129 555 Antisense
GAACCTTCTGTCCAGTTCTGACAAA 595 345 578 Antisense
TGCGATTCCAAAGCCGAATGCTCCA 263 583 603 Antisense
AATTGCAGCTTCTCCGACAAGTGCT 116 179 636 Antisense
GTGCTCAGATTATTCCGTTTTACGA 626 477 656 Antisense
Sequence Source Affymetrix Proprietary Database