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[178538_at]

Description F58B4.4 /REP_DB=WormBase Gene ID /WP=CE06014 /TR=Q20972 /GB=CAA98494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 8.5
(-2.5)
A 		4.3
(-3)
A 		15.7
(-1.7)
A 		58.3
(3)
A 		54
B 5.3
(-2.9)
A 		5.1
(-3)
A 		16.5
(3)
A 		12.2
(0.7)
A 		11
C 22.7
(2.1)
A 		26.2
(3)
A 		3.5
(-3)
A 		19
(1.1)
A 		23
avr 12.2
(-2.9) 		11.9
(-3) 		11.9
(-3) 		29.8
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 9.5
(-0.8)
A 		10.5
(-0.4)
A 		16
(1.8)
A 		5.6
(-2.4)
A 		19.1
(3)
A 		4.1
(-3)
A 		15
B 32.9
(3)
A 		32.9
(3)
A 		3.4
(-3)
A 		7.2
(-2.2)
A 		27.7
(1.9)
A 		5.8
(-2.5)
A 		30
C 5.7
(-2.7)
A 		36.8
(3)
A 		3.9
(-3)
A 		4.5
(-2.9)
A 		17.2
(-0.6)
A 		4.3
(-2.9)
A 		33
avr 16
(0.1) 		26.7
(3) 		7.8
(-2.2) 		5.8
(-2.7) 		21.3
(1.5) 		4.7
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 3.1
(-1.3)
A 		0.9
(-3)
A 		4.1
(-0.5)
A 		8.5
(3)
A 		8
B 1.7
(-2)
A 		1.3
(-3)
A 		3.8
(3)
A 		2.6
(0.1)
A 		3
C 7.5
(3)
A 		7.5
(3)
A 		1.2
(-3)
A 		3.2
(-1.1)
A 		6
avr 4.1
(0.7) 		3.2
(-2.3) 		3
(-3) 		4.8
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.8
(-2)
A 		3.7
(0.4)
A 		3.5
(0.1)
A 		1.4
(-2.5)
A 		5.8
(3)
A 		1
(-3)
A 		5
B 11.4
(3)
A 		10.9
(2.7)
A 		0.8
(-3)
A 		1.9
(-2.4)
A 		11.3
(2.9)
A 		2.2
(-2.2)
A 		11
C 2.1
(-2.3)
A 		11.2
(3)
A 		1
(-2.9)
A 		0.9
(-3)
A 		5.6
(-0.3)
A 		1.4
(-2.7)
A 		10
avr 5.1
(0.1) 		8.6
(3) 		1.8
(-2.7) 		1.4
(-3) 		7.6
(2.2) 		1.5
(-2.9) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178538_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14029
Sequence Type Exemplar sequence
Representative Public ID F58B4.4 NCBI
Target Description F58B4.4 /REP_DB=WormBase Gene ID /WP=CE06014 /TR=Q20972 /GB=CAA98494.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073386(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073386 NCBI Caenorhabditis elegans F58B4.4 (F58B4.4) mRNA, complete cds. 11/11 None
SNAP00000017999 ENSEMBL cdna:SNAP chromosome:CEL140:V:10933365:10933876:-1 11/11 None
GENEFINDER00000018003 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10933365:10933876:-1 11/11 None
F58B4.4 ENSEMBL cdna:known chromosome:CEL140:V:10933365:10933876:-1 gene:F58B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10933364-10933876(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58B4.4
Entrez Gene 186496 Entrez gene
SwissProt Q20972 EMBL-EBI
WormBase CE06014 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98494 Hypothetical protein F58B4.4 [Caenorhabditis elegans] ref|NP_505787.1| F58B4.4 [Caenorhabditis elegans] 1.0E-73
blast CAE64912 Hypothetical protein CBG09732 [Caenorhabditis briggsae] 1.0E-56
Sequence
Target Sequence 
>C. ELEGANS:178538_AT
catcctatatactactatttgcttctgtagtctgcttgggcttttgtgctttggacgacc
aacttttgacggaagaaggaagtggatttgagccaccgccgattgtcaatataactgtgg
agttggtatcaaatagatcttttgatccggaaaagaatgcttccaatttgaaaattgtga
aagatgctgttgatgattattccaaaaaggagaatattaggtacccatctgacaatatca
aacaatcaattattgatcacggaggaaagtttgcagttctttttgagatccgtgatgacg
caagatgtgatgaagttcgccgttttgttaaaggagcaaagaagcactcaaatgaacttt
catatgctgttctgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATCCTATATACTACTATTTGCTTC 581 211 20 Antisense
ATTTGCTTCTGTAGTCTGCTTGGGC 701 17 36 Antisense
TGTAGTCTGCTTGGGCTTTTGTGCT 525 559 45 Antisense
TTGGGCTTTTGTGCTTTGGACGACC 505 699 55 Antisense
TTGGACGACCAACTTTTGACGGAAG 554 707 70 Antisense
AGCCACCGCCGATTGTCAATATAAC 550 83 110 Antisense
TTAGGTACCCATCTGACAATATCAA 440 663 236 Antisense
GTTTGCAGTTCTTTTTGAGATCCGT 121 451 288 Antisense
GAGATCCGTGATGACGCAAGATGTG 509 395 304 Antisense
GTGATGAAGTTCGCCGTTTTGTTAA 317 483 326 Antisense
ATGAACTTTCATATGCTGTTCTGAA 309 45 371 Antisense
Sequence Source Affymetrix Proprietary Database