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[178519_at]

Description C35A5.4 /REP_DB=WormBase Gene ID /WP=CE05362 /TR=Q18471 /GB=CAA94905.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 5.5
(-3)
A 		8.7
(-2.6)
A 		13
(-2.1)
A 		54
(3)
A 		49
B 14.8
(3)
A 		6.2
(-2.7)
A 		5.8
(-3)
A 		9
(-0.9)
A 		9
C 6.4
(-3)
A 		8
(-2.9)
A 		5.9
(-3)
A 		121.1
(3)
A 		115
avr 8.9
(-2.9) 		7.6
(-3) 		8.2
(-2.9) 		61.4
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 5.8
(-3)
A 		6.4
(-2.9)
A 		7.1
(-2.8)
A 		6
(-3)
A 		29
(0.6)
A 		44.4
(3)
A 		39
B 4.1
(-3)
A 		4.6
(-2.9)
A 		5.3
(-2.7)
A 		11.9
(-1.2)
A 		30.5
(3)
A 		11
(-1.4)
A 		26
C 5.3
(-3)
A 		16.1
(-1.2)
A 		5.8
(-2.9)
A 		14.4
(-1.5)
A 		39.3
(2.7)
A 		41.4
(3)
A 		36
avr 5.1
(-3) 		9
(-2.2) 		6.1
(-2.8) 		10.8
(-1.8) 		32.9
(3) 		32.3
(2.9) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 2
(-2.9)
A 		1.9
(-3)
A 		3.4
(-1.5)
A 		7.9
(3)
A 		6
B 4.7
(3)
A 		1.5
(-2.6)
A 		1.3
(-3)
A 		1.9
(-1.9)
A 		3
C 2.1
(-3)
A 		2.3
(-2.9)
A 		2.1
(-3)
A 		20.4
(3)
A 		18
avr 2.9
(-2.3) 		1.9
(-3) 		2.3
(-2.7) 		10.1
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1.1
(-3)
A 		2.3
(-2.3)
A 		1.5
(-2.8)
A 		1.5
(-2.8)
A 		8.7
(1.7)
A 		10.8
(3)
A 		10
B 1.4
(-2.9)
A 		1.5
(-2.9)
A 		1.3
(-3)
A 		3.1
(-2)
A 		12.4
(3)
A 		4.1
(-1.5)
A 		11
C 2
(-2.8)
A 		4.9
(-1.4)
A 		1.5
(-3)
A 		2.9
(-2.3)
A 		12.7
(2.4)
A 		13.9
(3)
A 		12
avr 1.5
(-2.9) 		2.9
(-2.1) 		1.4
(-3) 		2.5
(-2.3) 		11.3
(3) 		9.6
(2) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178519_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12478
Sequence Type Exemplar sequence
Representative Public ID C35A5.4 NCBI
Target Description C35A5.4 /REP_DB=WormBase Gene ID /WP=CE05362 /TR=Q18471 /GB=CAA94905.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073292(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073292 NCBI Caenorhabditis elegans C35A5.4 (C35A5.4) mRNA, complete cds. 11/11 None
SNAP00000025023 ENSEMBL cdna:SNAP chromosome:CEL140:V:10499667:10501906:1 11/11 None
GENEFINDER00000025051 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10499667:10501906:1 11/11 None
C35A5.4 ENSEMBL cdna:known chromosome:CEL140:V:10499667:10501906:1 gene:C35A5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10499666-10501906(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C35A5.4
Entrez Gene 183222 Entrez gene
SwissProt Q18471 EMBL-EBI
WormBase CE35422 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94905 Hypothetical protein C35A5.4 [Caenorhabditis elegans] ref|NP_505693.2| C35A5.4 [Caenorhabditis elegans] 0.0
blast CAE69123 Hypothetical protein CBG15149 [Caenorhabditis briggsae] 2.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 7.3E-7
Sequence
Target Sequence 
>C. ELEGANS:178519_AT
cgaagaagcccataatacgccgtccatcgccactacgtgctcgttccaaaactccaggat
ccaaggataacaaaaatcagcgtgataagcgtgaaggtggagtaatgaagatgtttcgaa
atctcaatccatttgatagaactccgactccgactacaaagaagacacaatcggttacgc
ccagtaatagtactccgtctccagcaaagccagccactccaacaaaaacgccaacaccga
ctaagaagcctaatgagaccctatctcaagtcaagacagtttccaaggcactgtctcatt
ctcccaagtcaccaaagaagaacagtcaggaaaagtcggctccaccatcaccgaagttac
caccgaaaactacagcaccaacgccaacaaaagacggtggcaatcattctccaaaaacga
ctaacaaggcattagagccgccgccgagcccaaatcgtacacattcgccaagtgcatacc
aacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAGAAGCCCATAATACGCCGTCC 405 251 773 Antisense
TGCTCGTTCCAAAACTCCAGGATCC 631 581 810 Antisense
CCATTTGATAGAACTCCGACTCCGA 299 271 901 Antisense
AGAAGACACAATCGGTTACGCCCAG 432 73 932 Antisense
TACGCCCAGTAATAGTACTCCGTCT 389 645 948 Antisense
AACGCCAACACCGACTAAGAAGCCT 365 145 999 Antisense
CAGTTTCCAAGGCACTGTCTCATTC 42 207 1049 Antisense
AGTCAGGAAAAGTCGGCTCCACCAT 326 61 1096 Antisense
AAGACGGTGGCAATCATTCTCCAAA 305 153 1163 Antisense
CGAGCCCAAATCGTACACATTCGCC 604 249 1217 Antisense
CACATTCGCCAAGTGCATACCAACC 220 197 1232 Antisense
Sequence Source Affymetrix Proprietary Database