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[178494_at]

Description C27D8.3 /REP_DB=WormBase Gene ID /WP=CE08422 /TR=Q93290 /GB=CAB02263.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 60      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 72      6grp_total > 41      4grp_total > 15      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 802
1 2 3 4 range
A 1036
(2.8)
P 		364.3
(-3)
P 		529.6
(-1.6)
P 		1056.8
(3)
P 		693
B 997.6
(1.7)
P 		397.2
(-3)
P 		513.7
(-2.1)
P 		1166.7
(3)
P 		770
C 1101.2
(3)
P 		390.9
(-3)
P 		532.1
(-1.8)
P 		938.2
(1.6)
P 		710
avr 1044.9
(2.9) 		384.1
(-3) 		525.1
(-1.7) 		1053.9
(3) 		670

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 442
(2.1)
P 		463.1
(3)
P 		396.3
(0)
P 		438
(1.9)
P 		415.5
(0.9)
P 		328.8
(-3)
P 		134
B 466.4
(-0.2)
P 		453.8
(-0.6)
P 		449.6
(-0.7)
P 		577.1
(3)
P 		371.7
(-3)
P 		377.3
(-2.8)
P 		205
C 398.2
(-1.1)
P 		420.1
(-0.1)
P 		489.3
(3)
P 		395.8
(-1.3)
P 		433.5
(0.5)
P 		357.4
(-3)
P 		132
avr 435.5
(1.2) 		445.7
(1.7) 		445.1
(1.7) 		470.3
(3) 		406.9
(-0.3) 		354.5
(-3) 		116
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 298
1 2 3 4 range
A 377.2
(3)
P 		79.1
(-3)
P 		138.5
(-1.8)
P 		154.7
(-1.5)
P 		298
B 318.1
(3)
P 		98.1
(-3)
P 		119
(-2.4)
P 		248.6
(1.1)
P 		220
C 364.5
(3)
P 		112.4
(-3)
P 		185.5
(-1.3)
P 		158.2
(-1.9)
P 		252
avr 353.3
(3) 		96.5
(-3) 		147.7
(-1.8) 		187.2
(-0.9) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 81.5
(-2.9)
P 		164.7
(3)
P 		86.3
(-2.6)
P 		111.1
(-0.8)
P 		125.4
(0.2)
P 		80.1
(-3)
P 		85
B 162.3
(3)
P 		151
(1.7)
P 		109.2
(-3)
P 		152.9
(1.9)
P 		151
(1.7)
P 		140.7
(0.6)
P 		53
C 147.6
(3)
P 		128.2
(1.3)
P 		124.5
(1)
P 		79.6
(-3)
P 		140.3
(2.4)
P 		119.6
(0.5)
P 		68
avr 130.5
(0.5) 		148
(3) 		106.7
(-3) 		114.5
(-1.9) 		138.9
(1.7) 		113.5
(-2) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178494_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8546
Sequence Type Exemplar sequence
Representative Public ID C27D8.3 NCBI
Target Description C27D8.3 /REP_DB=WormBase Gene ID /WP=CE08422 /TR=Q93290 /GB=CAB02263.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070025(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070025 NCBI Caenorhabditis elegans C27D8.3 (C27D8.3) mRNA, complete cds. 11/11 None
SNAP00000003311 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12706888:12711704:-1 11/11 None
GENEFINDER00000003314 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12706888:12711704:-1 11/11 None
C27D8.3 ENSEMBL cdna:known chromosome:CEL140:IV:12706693:12712285:-1 gene:C27D8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12706893-12712291(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27D8.3
Entrez Gene 178224 Entrez gene
SwissProt Q93290 EMBL-EBI
WormBase CE08422 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02263 Hypothetical protein C27D8.3 [Caenorhabditis elegans] ref|NP_502426.1| C27D8.3 [Caenorhabditis elegans] 0.0
blast CAE56980 Hypothetical protein CBG24833 [Caenorhabditis briggsae] 0.0
blast CAE59996 Hypothetical protein CBG03489 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:178494_AT
tacacatgtcaaattcacgcctctccgtgtcgcggttgttgcctgccgtgatcagacacc
tcgtccatcaccgcgaccttccgaatcgcacgatcttccgacgtcatcatcctcggttca
tgctccatcggaatgtgttttcactgcgccgaatcttccccaacgataccatcagaatca
aaatcacgacccattgatgtcaatgatgatgatgacgtcatcatcttcatccacgtcatc
accaacaaacacaacaccaacaatgatgacacctggaccaatggatcaatcattcgaaat
gcaattggaggctctaatgaaacaatacggaaagactccgcatgatgtctgcgaaatagt
gtactccgctgcaagacccaataataatctaaatcaacaacaacaacatcatcatcaatc
ccatcaccatccatcactaattccaccaacaaccacatcaattcgttctccaacattagg
tcctccctcatctcaggatagaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACACATGTCAAATTCACGCCTCTC 278 643 2367 Antisense
ATCGGAATGTGTTTTCACTGCGCCG 73 35 2493 Antisense
TCTTCCCCAACGATACCATCAGAAT 619 613 2520 Antisense
AAATCACGACCCATTGATGTCAATG 252 117 2547 Antisense
GTCATCACCAACAAACACAACACCA 487 467 2601 Antisense
GATGACACCTGGACCAATGGATCAA 95 411 2631 Antisense
GAAAGACTCCGCATGATGTCTGCGA 467 359 2696 Antisense
GAAATAGTGTACTCCGCTGCAAGAC 661 361 2719 Antisense
AACAACCACATCAATTCGTTCTCCA 441 135 2814 Antisense
AATTCGTTCTCCAACATTAGGTCCT 614 175 2826 Antisense
GTCCTCCCTCATCTCAGGATAGAAG 310 475 2846 Antisense
Sequence Source Affymetrix Proprietary Database