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[178493_at]

Description F28H7.1 /REP_DB=WormBase Gene ID /WP=CE15924 /TR=Q19889 /GB=CAA96641.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 56      4grp_total > 10      6grp_cgc > 55      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 211
1 2 3 4 range
A 3.3
(-3)
A 		26.5
(-2.1)
A 		20.2
(-2.4)
A 		166.4
(3)
P 		163
B 43.5
(-3)
A 		46
(-2.9)
A 		48.9
(-2.8)
A 		214
(3)
P 		171
C 11.8
(-3)
A 		30.4
(-2.4)
A 		19.5
(-2.8)
A 		197.5
(3)
P 		186
avr 19.5
(-3) 		34.3
(-2.5) 		29.5
(-2.7) 		192.6
(3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 23.2
(-0.4)
A 		21
(-0.8)
A 		47.5
(3)
A 		5.2
(-3)
A 		9.1
(-2.4)
A 		31.8
(0.8)
A 		42
B 6.7
(-2.5)
A 		35.8
(1.5)
A 		23.1
(-0.2)
A 		2.9
(-3)
A 		38.5
(1.9)
A 		46.4
(3)
A 		44
C 15.2
(-2)
A 		61
(3)
A 		5.8
(-3)
A 		8.4
(-2.7)
A 		11
(-2.4)
A 		54.9
(2.3)
A 		55
avr 15
(-1.5) 		39.3
(2.2) 		25.5
(0.1) 		5.5
(-3) 		19.5
(-0.8) 		44.4
(3) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 1.2
(-3)
A 		5.7
(-1.8)
A 		5.3
(-1.9)
A 		24.4
(3)
P 		23
B 13.9
(-2.5)
A 		11.4
(-3)
A 		11.3
(-3)
A 		45.6
(3)
P 		34
C 3.9
(-3)
A 		8.7
(-2)
A 		6.8
(-2.4)
A 		33.3
(3)
P 		29
avr 6.3
(-3) 		8.6
(-2.5) 		7.8
(-2.7) 		34.4
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 4.3
(-1)
A 		7.5
(1.1)
A 		10.3
(3)
A 		1.3
(-3)
A 		2.8
(-2)
A 		7.7
(1.3)
A 		9
B 2.3
(-2.5)
A 		11.9
(1)
A 		5.6
(-1.3)
A 		0.8
(-3)
A 		15.6
(2.4)
A 		17.3
(3)
A 		17
C 5.6
(-1.6)
A 		18.6
(3)
A 		1.5
(-3)
A 		1.7
(-2.9)
A 		3.6
(-2.3)
A 		18.4
(2.9)
A 		17
avr 4.1
(-1.7) 		12.7
(2.2) 		5.8
(-1) 		1.3
(-3) 		7.3
(-0.3) 		14.5
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178493_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12550
Sequence Type Exemplar sequence
Representative Public ID F28H7.1 NCBI
Target Description F28H7.1 /REP_DB=WormBase Gene ID /WP=CE15924 /TR=Q19889 /GB=CAA96641.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073338(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073338 NCBI Caenorhabditis elegans Serpentine Receptor, class J family member (srj-22) (srj-22) mRNA, complete cds. 11/11 None
SNAP00000038689 ENSEMBL cdna:SNAP chromosome:CEL140:V:10745051:10746334:-1 11/11 None
GENEFINDER00000038699 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10745051:10746334:-1 11/11 None
F28H7.1 ENSEMBL cdna:known chromosome:CEL140:V:10745051:10746334:-1 gene:F28H7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10745050-10746334(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srj-22
Ensembl F28H7.1
Entrez Gene 185095 Entrez gene
SwissProt Q19889 EMBL-EBI
WormBase CE15924 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA96641 Hypothetical protein F28H7.1 [Caenorhabditis elegans] ref|NP_505739.1| Serpentine Receptor, class J family member (srj-22) [Caenorhabditis elegans] 0.0
blast CAE71082 Hypothetical protein CBG17931 [Caenorhabditis briggsae] 1.0E-161
blast CAA96641 Hypothetical protein F28H7.1 [Caenorhabditis elegans] ref|NP_505739.1| Serpentine Receptor, class J family member (srj-22) [Caenorhabditis elegans] 1.0E-111
blast NP_001024073 Serpentine Receptor, class J family member (srj-23) [Caenorhabditis elegans] gb|AAX22285.1| Serpentine receptor, class j protein 23, isoform b [Caenorhabditis elegans] 3.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 5.3E-6
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 5.3E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505739.1 7 10-30,43-65,91-113,126-148,196-218,247-269,279-301
F28H7.1 7 10-30,43-65,91-113,126-148,196-218,247-269,279-301
SNAP00000038689 6 13-30,40-62,83-102,112-134,162-184,194-216
GENEFINDER00000038699 7 10-30,43-65,91-113,126-148,196-218,247-269,279-301
Sequence
Target Sequence 
>C. ELEGANS:178493_AT
gatcgacgtctatacattcgtgaatcaatgtttgagcatcatggagtagatgttatgaat
attactataatcattgctcaatattttgaaggaactccaaatgccatgcgattatcaaga
attggaattggatctatgtcagtactgtctataatttcacttgctttcattttctacttc
ggatacaaaatatgccacaaattaagcagtcagagttctgatatgagtgaaaaaactaaa
aaacttcaaactcaattaatgaaagctctgactgttcaagcaataattccgacttgtgtg
agttttgcgccatgcctctttgcttggtatcaaccagtttttggattggatttgggaaga
tggattcaatttgctgctggaattgctgtggccacatttccagcccttgatccactggct
ctcatttattttgtaccaacgttccggagaaagtttatagaaaagttgatgtttttcaag
tttacgaaaaagaatcaaacagcgtgctctcaagtatccaatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGACGTCTATACATTCGTGAAT 547 417 487 Antisense
GAAGGAACTCCAAATGCCATGCGAT 706 333 574 Antisense
ATTGGATCTATGTCAGTACTGTCTA 337 3 613 Antisense
ACTGTCTATAATTTCACTTGCTTTC 569 97 630 Antisense
CTTGCTTTCATTTTCTACTTCGGAT 116 219 646 Antisense
GACTTGTGTGAGTTTTGCGCCATGC 529 371 777 Antisense
TGCCTCTTTGCTTGGTATCAACCAG 451 585 799 Antisense
AATTGCTGTGGCCACATTTCCAGCC 632 177 867 Antisense
CTTGATCCACTGGCTCTCATTTATT 113 219 892 Antisense
GGCTCTCATTTATTTTGTACCAACG 607 539 903 Antisense
CAGCGTGCTCTCAAGTATCCAATAT 598 203 986 Antisense
Sequence Source Affymetrix Proprietary Database