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[178477_at]

Description F28G4.3 /REP_DB=WormBase Gene ID /WP=CE15921 /TR=O17853 /GB=CAB07606.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 2.7
(-3)
A 		21.7
(0.3)
A 		3.1
(-2.9)
A 		36.8
(3)
A 		34
B 5.1
(-3)
A 		18.5
(3)
A 		12.1
(0.1)
A 		13.9
(0.9)
A 		13
C 27.7
(-0.6)
A 		5.5
(-2.6)
A 		1.4
(-3)
A 		68
(3)
A 		67
avr 11.8
(-1.9) 		15.2
(-1.3) 		5.5
(-3) 		39.6
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 5.1
(-1.8)
A 		9.9
(-0.4)
A 		0.8
(-3)
A 		3.5
(-2.2)
A 		12.8
(0.4)
A 		22
(3)
A 		21
B 1.8
(-3)
A 		2.3
(-2.9)
A 		9.3
(-1.5)
A 		31.9
(3)
A 		16.5
(-0.1)
A 		16.2
(-0.1)
A 		30
C 1.6
(-3)
A 		18.3
(1.6)
A 		23.2
(3)
A 		4.7
(-2.1)
A 		2.7
(-2.7)
A 		3.1
(-2.6)
A 		22
avr 2.8
(-3) 		10.2
(1) 		11.1
(1.5) 		13.4
(2.8) 		10.7
(1.3) 		13.8
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1
(-2.7)
A 		4.7
(2.1)
A 		0.8
(-3)
A 		5.4
(3)
A 		5
B 1.6
(-3)
A 		4.6
(3)
A 		2.8
(-0.6)
A 		3
(-0.2)
A 		3
C 9.2
(1.7)
A 		1.6
(-2.4)
A 		0.5
(-3)
A 		11.5
(3)
A 		11
avr 3.9
(-0.1) 		3.6
(-0.5) 		1.4
(-3) 		6.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.9
(-2.2)
A 		3.5
(0.8)
A 		0.2
(-3)
A 		0.9
(-2.2)
A 		3.9
(1.3)
A 		5.4
(3)
A 		5
B 0.6
(-3)
A 		0.8
(-2.8)
A 		2.3
(-1.7)
A 		8.5
(3)
A 		6.7
(1.6)
A 		6
(1.1)
A 		8
C 0.6
(-3)
A 		5.6
(2.7)
A 		5.9
(3)
A 		0.9
(-2.7)
A 		0.9
(-2.7)
A 		1
(-2.5)
A 		5
avr 0.7
(-3) 		3.3
(1.6) 		2.8
(0.7) 		3.4
(1.8) 		3.8
(2.5) 		4.1
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178477_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12896
Sequence Type Exemplar sequence
Representative Public ID F28G4.3 NCBI
Target Description F28G4.3 /REP_DB=WormBase Gene ID /WP=CE15921 /TR=O17853 /GB=CAB07606.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074711(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074711 NCBI Caenorhabditis elegans F28G4.3 (F28G4.3) mRNA, complete cds. 11/11 None
GENEFINDER00000032999 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16288734:16290302:-1 11/11 None
F28G4.3 ENSEMBL cdna:known chromosome:CEL140:V:16288734:16290302:-1 gene:F28G4.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032995 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16288733-16290302(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28G4.3
Entrez Gene 185081 Entrez gene
SwissProt O17853 EMBL-EBI
WormBase CE15921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07606 Hypothetical protein F28G4.3 [Caenorhabditis elegans] ref|NP_507112.1| F28G4.3 [Caenorhabditis elegans] 0.0
blast AAB18283 Hypothetical protein C16D9.5 [Caenorhabditis elegans] ref|NP_505125.2| C16D9.5 [Caenorhabditis elegans] 9.0E-91
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 5.0E-89
Sequence
Target Sequence 
>C. ELEGANS:178477_AT
gagcgaatatcgttttaagcctttaattgtggcacaagctctgaaagaattcccgaatat
ttggtggatggatgcagatgtgaatgtgaaaaagtcacacttgaaggagttgtttttcag
tgaattaaaatcaaatgcaacaattaaaaacggtgaaaacgaagaatttctgattcagat
atctcccatcaaattctttgattttactggccacagtaattatgccactttgtttctcga
tgttttaacctatttcccgaccaattccttaacactgctaaaaaatgagaagaacggcgt
tcagcttggcgcgaatacttttattctatctcggacgaaattttccatagaaattttgaa
atggtgggtgctctgcgcacttgacaagacgtgtatgagccctcccggagcccaagttgc
atgccgtttcagcaaagatagatataatgactttgccaattgcttcagatttgatcaatc
tgtgctgaatcttctcatgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGAATATCGTTTTAAGCCTTTA 194 387 537 Antisense
GATTCAGATATCTCCCATCAAATTC 288 431 708 Antisense
ATTATGCCACTTTGTTTCTCGATGT 666 13 755 Antisense
TCTCGATGTTTTAACCTATTTCCCG 375 611 771 Antisense
CGACCAATTCCTTAACACTGCTAAA 273 251 794 Antisense
CGTTCAGCTTGGCGCGAATACTTTT 519 247 834 Antisense
AATACTTTTATTCTATCTCGGACGA 238 161 850 Antisense
TTGAAATGGTGGGTGCTCTGCGCAC 520 703 892 Antisense
CCCAAGTTGCATGCCGTTTCAGCAA 195 257 947 Antisense
ATGACTTTGCCAATTGCTTCAGATT 109 45 983 Antisense
AATCTGTGCTGAATCTTCTCATGCT 323 169 1013 Antisense
Sequence Source Affymetrix Proprietary Database