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[178473_at]

Description F28H7.7 /REP_DB=WormBase Gene ID /WP=CE15925 /TR=Q19894 /GB=CAA96643.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 29      6grp_total > 01      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 40
(-3)
P 		69.4
(2.4)
A 		47.7
(-1.6)
P 		72.6
(3)
P 		33
B 31.5
(-2.3)
P 		63.5
(3)
P 		37.7
(-1.2)
P 		27
(-3)
A 		37
C 18.6
(-3)
A 		55.8
(3)
P 		23.4
(-2.2)
P 		37.9
(0.1)
P 		37
avr 30
(-3) 		62.9
(3) 		36.3
(-1.9) 		45.8
(-0.1) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 36.4
(2.2)
A 		39.3
(3)
P 		17.9
(-3)
A 		23.8
(-1.3)
A 		31.9
(0.9)
A 		24.8
(-1.1)
A 		21
B 33.8
(2.4)
P 		36.8
(3)
A 		35.5
(2.7)
P 		8.9
(-3)
A 		29
(1.3)
P 		16.1
(-1.5)
A 		28
C 13.8
(-3)
A 		44.6
(3)
P 		22.3
(-1.3)
A 		25.6
(-0.7)
A 		23.2
(-1.2)
M 		20.4
(-1.7)
P 		31
avr 28
(-0.5) 		40.2
(3) 		25.2
(-1.3) 		19.4
(-3) 		28
(-0.5) 		20.4
(-2.7) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 14.6
(2.3)
P 		15.1
(3)
A 		12.5
(-0.5)
P 		10.6
(-3)
P 		5
B 10.1
(-0.4)
P 		15.7
(3)
P 		8.7
(-1.2)
P 		5.8
(-3)
A 		10
C 6.2
(-3)
A 		16
(3)
P 		8.2
(-1.8)
P 		6.4
(-2.9)
P 		10
avr 10.3
(-1) 		15.6
(3) 		9.8
(-1.4) 		7.6
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 6.7
(-1.3)
A 		14
(3)
P 		3.9
(-3)
A 		6
(-1.8)
A 		9.6
(0.4)
A 		6
(-1.8)
A 		10
B 11.8
(2.8)
P 		12.2
(3)
A 		8.6
(0.8)
P 		2.4
(-3)
A 		11.8
(2.8)
P 		6
(-0.8)
A 		10
C 5.1
(-3)
A 		13.6
(3)
P 		5.7
(-2.6)
A 		5.1
(-3)
A 		7.5
(-1.3)
M 		6.8
(-1.8)
P 		9
avr 7.9
(-0.7) 		13.3
(3) 		6.1
(-1.9) 		4.5
(-3) 		9.6
(0.5) 		6.3
(-1.8) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178473_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13317
Sequence Type Exemplar sequence
Representative Public ID F28H7.7 NCBI
Target Description F28H7.7 /REP_DB=WormBase Gene ID /WP=CE15925 /TR=Q19894 /GB=CAA96643.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073343(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073343 NCBI Caenorhabditis elegans F28H7.7 (F28H7.7) mRNA, complete cds. 11/11 None
SNAP00000038693 ENSEMBL cdna:SNAP chromosome:CEL140:V:10755089:10756732:-1 9/11 None
GENEFINDER00000038707 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10755089:10756732:-1 9/11 None
F28H7.7 ENSEMBL cdna:known chromosome:CEL140:V:10753642:10756732:-1 gene:F28H7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10753641-10756732(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28H7.7
Entrez Gene 185098 Entrez gene
SwissProt Q19894 EMBL-EBI
WormBase CE15925 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA96643 Hypothetical protein F28H7.7 [Caenorhabditis elegans] ref|NP_505744.1| F28H7.7 [Caenorhabditis elegans] 0.0
blast CAE64880 Hypothetical protein CBG09692 [Caenorhabditis briggsae] 1.0E-152
blast CAE64880 Hypothetical protein CBG09692 [Caenorhabditis briggsae] 1.0E-140
blast NP_503534 T06A1.5 [Caenorhabditis elegans] gb|AAC17770.1| Hypothetical protein T06A1.5 [Caenorhabditis elegans] 1.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.2E-42
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 3.0E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505744.1 1 12-34
F28H7.7 1 12-34
SNAP00000038693 1 12-34
GENEFINDER00000038707 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:178473_AT
tattcatattccttcaatctactttgatgcagtctctcaacagtatctagcaattgcgga
ctgtcacttgcgaaaccaattatcagccaattggacaatcttttccgacattgacgagag
aatcattttgactgatagcaagacaactattcgaaattttttacaagattcagtcagtga
gaagtatggaggagttatgttcccacagcgttggatattcaaatatgaaaagctgcctga
aaagtttattaatccagtacagataatgcaagaaatgccgtcaaggaaatgggaacttac
aactcaaccatggctcaactgtacagatggaaaacattgcttctcaaaaatgatagtgaa
taatcaaaaagtattacaaatgatgattcatgacgtcggagagtataatgggaattatca
gactttgattcttgatccgaaaattggatatataaggcactatcgagatgtaaatatggg
aaaatggtgggtgagaaataaggacgtacttgaaaaactgaagccatacgaaaataccac
gtacaatttacgtctccgagaccaacttctaacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCATATTCCTTCAATCTACTTT 549 663 648 Antisense
CAATCTACTTTGATGCAGTCTCTCA 458 181 662 Antisense
GCAGTCTCTCAACAGTATCTAGCAA 22 313 676 Antisense
ATTGCGGACTGTCACTTGCGAAACC 680 5 700 Antisense
GGACAATCTTTTCCGACATTGACGA 51 527 740 Antisense
TGGAGGAGTTATGTTCCCACAGCGT 528 545 834 Antisense
GGAACTTACAACTCAACCATGGCTC 551 527 939 Antisense
TCAACCATGGCTCAACTGTACAGAT 710 631 951 Antisense
TCAGACTTTGATTCTTGATCCGAAA 458 623 1065 Antisense
ACCACGTACAATTTACGTCTCCGAG 576 85 1183 Antisense
GTCTCCGAGACCAACTTCTAACAAA 247 471 1199 Antisense
Sequence Source Affymetrix Proprietary Database