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[178461_at]

Description C35A5.9 /REP_DB=WormBase Gene ID /WP=CE05367 /TR=Q18477 /GB=CAA94910.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 32      4grp_total > 15      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 111.8
(3)
P 		32.8
(-3)
A 		46.2
(-2)
A 		110.4
(2.9)
A 		79
B 95.7
(1.6)
P 		28.1
(-3)
A 		63.4
(-0.6)
A 		115.5
(3)
A 		87
C 123.8
(2.8)
P 		56.9
(-2.5)
A 		49.9
(-3)
A 		126.3
(3)
A 		76
avr 110.4
(2.5) 		39.3
(-3) 		53.2
(-1.9) 		117.4
(3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 72.1
(-0.2)
A 		82.2
(0.3)
P 		75.4
(-0)
A 		139.5
(3)
A 		88.5
(0.6)
A 		12.6
(-3)
A 		127
B 96.8
(0.8)
A 		72.7
(-1.9)
A 		117
(3)
M 		97.1
(0.8)
A 		94.6
(0.5)
A 		62.8
(-3)
A 		54
C 133.4
(2.4)
M 		120.7
(1.7)
A 		142.3
(3)
P 		68.8
(-1.6)
A 		95.7
(0.1)
M 		45.7
(-3)
A 		97
avr 100.8
(2.1) 		91.9
(1.3) 		111.6
(3) 		101.8
(2.2) 		92.9
(1.4) 		40.4
(-3) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 40.7
(3)
P 		7.1
(-3)
A 		12.1
(-2.1)
A 		16.2
(-1.4)
A 		34
B 30.5
(3)
P 		6.9
(-3)
A 		14.7
(-1)
A 		24.6
(1.5)
A 		24
C 41
(3)
P 		16.4
(-3)
A 		17.4
(-2.8)
A 		21.3
(-1.8)
A 		25
avr 37.4
(3) 		10.1
(-3) 		14.7
(-2) 		20.7
(-0.7) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 13.3
(-1.1)
A 		29.2
(1.8)
P 		16.4
(-0.5)
A 		35.4
(3)
A 		26.7
(1.4)
A 		3.1
(-3)
A 		32
B 33.7
(1.1)
A 		24.2
(-2.7)
A 		28.4
(-1)
M 		25.7
(-2.1)
A 		38.5
(3)
A 		23.4
(-3)
A 		15
C 49.5
(3)
M 		36.8
(0.9)
A 		36.2
(0.8)
P 		13.8
(-3)
A 		31
(-0.1)
M 		15.3
(-2.7)
A 		36
avr 32.2
(3) 		30.1
(2.3) 		27
(1.3) 		25
(0.6) 		32.1
(3) 		13.9
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178461_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12194
Sequence Type Exemplar sequence
Representative Public ID C35A5.9 NCBI
Target Description C35A5.9 /REP_DB=WormBase Gene ID /WP=CE05367 /TR=Q18477 /GB=CAA94910.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073298(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073298 NCBI Caenorhabditis elegans C35A5.9 (C35A5.9) mRNA, complete cds. 11/11 None
SNAP00000025031 ENSEMBL cdna:SNAP chromosome:CEL140:V:10528891:10530977:1 11/11 None
GENEFINDER00000025049 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10529115:10530977:1 11/11 None
C35A5.9 ENSEMBL cdna:known chromosome:CEL140:V:10529115:10530977:1 gene:C35A5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10529114-10529849(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C35A5.9
Entrez Gene 183226 Entrez gene
SwissProt Q18477 EMBL-EBI
WormBase CE37881 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94910 Hypothetical protein C35A5.9 [Caenorhabditis elegans] ref|NP_505699.2| C35A5.9 [Caenorhabditis elegans] 1.0E-158
blast CAE64834 Hypothetical protein CBG09630 [Caenorhabditis briggsae] 1.0E-156
blast CAA94910 Hypothetical protein C35A5.9 [Caenorhabditis elegans] ref|NP_505699.2| C35A5.9 [Caenorhabditis elegans] 1.0E-151
blast CAE64834 Hypothetical protein CBG09630 [Caenorhabditis briggsae] 1.0E-124
blast XP_414321 PREDICTED: similar to Histone deacetylase 11 [Gallus gallus] 1.0E-78
blast XP_414321 PREDICTED: similar to Histone deacetylase 11 [Gallus gallus] 3.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000286 EMBL-EBI Histone deacetylase superfamily 2.5E-16
Pfam IPR000286 EMBL-EBI Histone deacetylase superfamily 8.0E-9
Sequence
Target Sequence 
>C. ELEGANS:178461_AT
cactcgttgagccaaatctaccgacatttgaagaactcactagagtacatgatagaaaat
atctgaaaagtgttcgaaatcctattaaagcagcacaaatcgtcgagattccatttgtcg
gttgccttccaccgtgcattattgaaagcaagttattgcatccactacgtttacaagccg
gtggaacagttttagctgccaatttggctctaaaacatggttgggcgatcaacgtgggtg
gtggtttccatcatgcttctcattcgggcggaggtggattctgtttttacgctgatatca
ccatggcaattttcgatttgtttgacaaaaaggcaattgccaacgcgattgttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTCGTTGAGCCAAATCTACCGAC 226 195 35 Antisense
CGAGATTCCATTTGTCGGTTGCCTT 150 251 138 Antisense
TTGCCTTCCACCGTGCATTATTGAA 352 701 156 Antisense
AGCAAGTTATTGCATCCACTACGTT 645 75 181 Antisense
GCATCCACTACGTTTACAAGCCGGT 52 309 192 Antisense
GCCGGTGGAACAGTTTTAGCTGCCA 575 283 211 Antisense
AACGTGGGTGGTGGTTTCCATCATG 74 143 265 Antisense
TGCTTCTCATTCGGGCGGAGGTGGA 481 579 288 Antisense
GTGGATTCTGTTTTTACGCTGATAT 577 489 308 Antisense
GCTGATATCACCATGGCAATTTTCG 486 295 325 Antisense
AGGCAATTGCCAACGCGATTGTTGT 361 55 365 Antisense
Sequence Source Affymetrix Proprietary Database