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[178451_at]

Description E03H4.5 /REP_DB=WormBase Gene ID /WP=CE09132 /TR=O17745 /GB=CAB04027.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 16      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 0.7
(-3)
A 		1.2
(-2.9)
A 		33.8
(3)
A 		27.2
(1.8)
A 		33
B 3.7
(-2.3)
A 		3.2
(-2.4)
A 		0.9
(-3)
A 		23.8
(3)
A 		23
C 0.8
(-3)
A 		13.8
(-1.4)
A 		4.2
(-2.6)
A 		48.8
(3)
P 		48
avr 1.7
(-3) 		6.1
(-2.2) 		13
(-0.9) 		33.3
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 19.6
(3)
A 		12
(0.6)
A 		5
(-1.7)
A 		2.5
(-2.5)
A 		18.7
(2.7)
A 		0.9
(-3)
A 		19
B 4
(-1.7)
A 		9.3
(0.2)
A 		6.5
(-0.8)
A 		0.5
(-3)
A 		17.2
(3)
A 		6.5
(-0.8)
A 		17
C 16.2
(3)
A 		4
(-2.2)
A 		4.8
(-1.8)
A 		2
(-3)
A 		13.6
(1.9)
A 		4.5
(-1.9)
A 		14
avr 13.3
(1.7) 		8.4
(-0.3) 		5.4
(-1.5) 		1.7
(-3) 		16.5
(3) 		4
(-2.1) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 0.3
(-3)
A 		0.3
(-3)
A 		8.8
(3)
A 		4
(-0.4)
A 		9
B 1.2
(-1.8)
A 		0.8
(-2.3)
A 		0.2
(-3)
A 		5.1
(3)
A 		5
C 0.3
(-3)
A 		4
(-0.2)
A 		1.5
(-2.1)
A 		8.2
(3)
P 		8
avr 0.6
(-3) 		1.7
(-1.7) 		3.5
(0.3) 		5.8
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 3.6
(0.8)
A 		4.3
(1.6)
A 		1.1
(-2)
A 		0.6
(-2.6)
A 		5.6
(3)
A 		0.2
(-3)
A 		5
B 1.4
(-1.9)
A 		3.1
(-0.4)
A 		1.6
(-1.7)
A 		0.1
(-3)
A 		7
(3)
A 		2.4
(-1)
A 		7
C 6
(3)
A 		1.2
(-2.1)
A 		1.2
(-2.1)
A 		0.4
(-3)
A 		4.4
(1.3)
A 		1.5
(-1.8)
A 		6
avr 3.7
(0.7) 		2.9
(-0.2) 		1.3
(-2) 		0.4
(-3) 		5.7
(3) 		1.4
(-1.9) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178451_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.386
Sequence Type Exemplar sequence
Representative Public ID E03H4.5 NCBI
Target Description E03H4.5 /REP_DB=WormBase Gene ID /WP=CE09132 /TR=O17745 /GB=CAB04027.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060747(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060747 NCBI Caenorhabditis elegans E03H4.5 (E03H4.5) mRNA, complete cds. 11/11 None
SNAP00000031038 ENSEMBL cdna:SNAP chromosome:CEL140:I:12421930:12424846:1 11/11 None
GENEFINDER00000031052 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12421930:12423866:1 11/11 None
E03H4.5 ENSEMBL cdna:known chromosome:CEL140:I:12421930:12423866:1 gene:E03H4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12421860-12423797(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E03H4.5
Entrez Gene 184020 Entrez gene
SwissProt O17745 EMBL-EBI
WormBase CE09132 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04027 Hypothetical protein E03H4.5 [Caenorhabditis elegans] ref|NP_493148.1| E03H4.5 [Caenorhabditis elegans] 0.0
blast CAB05625 Hypothetical protein T15D6.12 [Caenorhabditis elegans] ref|NP_493143.1| T15D6.12 [Caenorhabditis elegans] 0.0
blast AAC17753 Hypothetical protein C18G1.6 [Caenorhabditis elegans] ref|NP_504276.1| C18G1.6 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.0E-126
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493148.1 1 7-27
E03H4.5 1 7-27
SNAP00000031038 1 7-27
GENEFINDER00000031052 1 7-27
Sequence
Target Sequence 
>C. ELEGANS:178451_AT
ctactatcggatcagttgcggattttgatgaagttatggtacctcacaatggtacaatgt
tggaatatgcatcaaaggaaatgactggaacaaatgttggagcattattatttaagaatt
cctacgtttcactggaacccagcatttatgacaatggattttctggagtcttgaagccag
tgttcctagatggtatgggccctccgaagtacgttttcaatgcctctgtaattgacattg
gtcaagttcattgggttcgaagtttcatggattctacaaaacatactaaagtttccgatg
gtacactgttgcatcatcgttttgatgcaaaatggaaaagaggagaggaagtggaaaaac
tgttcacatacttccctaataacactttagagcacgttgcgagtatgcaaaagactgcta
tgacaatattcaatggatcgcttccctttttttcacttgatttaatcaatggtgtaaaca
attgcgttctgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACTATCGGATCAGTTGCGGATTT 546 223 791 Antisense
ACCCAGCATTTATGACAATGGATTT 565 89 927 Antisense
TGGATTTTCTGGAGTCTTGAAGCCA 666 549 945 Antisense
ATGCCTCTGTAATTGACATTGGTCA 444 41 1010 Antisense
TTGACATTGGTCAAGTTCATTGGGT 609 703 1022 Antisense
TTCATTGGGTTCGAAGTTTCATGGA 485 687 1037 Antisense
GGGTTCGAAGTTTCATGGATTCTAC 200 495 1043 Antisense
CACATACTTCCCTAATAACACTTTA 354 195 1155 Antisense
ATAACACTTTAGAGCACGTTGCGAG 435 25 1169 Antisense
CTTTAGAGCACGTTGCGAGTATGCA 238 219 1175 Antisense
TGGTGTAAACAATTGCGTTCTGCAA 529 553 1260 Antisense
Sequence Source Affymetrix Proprietary Database