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[178449_at]

Description C53B4.8 /REP_DB=WormBase Gene ID /WP=CE20601 /TR=Q9XTI8 /GB=CAA92458.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 82      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 65
(-3)
A 		119.1
(-0.3)
A 		184
(3)
A 		116
(-0.4)
A 		119
B 87.7
(-0.9)
A 		131
(3)
P 		115.9
(1.6)
P 		64.7
(-3)
A 		66
C 64.3
(-3)
A 		143.6
(2.7)
P 		147.4
(3)
P 		126.5
(1.5)
A 		83
avr 72.3
(-3) 		131.2
(1.6) 		149.1
(3) 		102.4
(-0.6) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 71.7
(-2.9)
A 		98.3
(-1.4)
P 		90.4
(-1.9)
A 		156.2
(1.9)
A 		175.8
(3)
M 		70.6
(-3)
A 		105
B 59.8
(-3)
A 		117.8
(1.4)
P 		73.9
(-1.9)
A 		139.3
(3)
P 		122.8
(1.8)
P 		83.5
(-1.2)
A 		80
C 84.8
(-3)
A 		177.1
(3)
P 		94.6
(-2.4)
A 		126.3
(-0.3)
A 		114.4
(-1.1)
A 		93
(-2.5)
A 		92
avr 72.1
(-3) 		131.1
(2.2) 		86.3
(-1.8) 		140.6
(3) 		137.7
(2.7) 		82.4
(-2.1) 		69
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 23.7
(-1.7)
A 		25.9
(-1.3)
A 		48.1
(3)
A 		17
(-3)
A 		31
B 28
(1.6)
A 		32.4
(3)
P 		26.9
(1.2)
P 		13.8
(-3)
A 		19
C 21.3
(-3)
A 		41.3
(1)
P 		51.4
(3)
P 		21.3
(-3)
A 		30
avr 24.3
(-1.3) 		33.2
(0.8) 		42.1
(3) 		17.4
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 13.2
(-3)
A 		35
(0.3)
P 		19.7
(-2)
A 		39.6
(1)
A 		53
(3)
M 		17.2
(-2.4)
A 		40
B 20.8
(-2.5)
A 		39.2
(1)
P 		18
(-3)
A 		36.9
(0.6)
P 		49.9
(3)
P 		31.1
(-0.5)
A 		32
C 31.4
(-1.5)
A 		54
(3)
P 		24.1
(-3)
A 		25.4
(-2.7)
A 		37
(-0.4)
A 		31.1
(-1.6)
A 		30
avr 21.8
(-2.7) 		42.7
(2.1) 		20.6
(-3) 		34
(0.1) 		46.6
(3) 		26.5
(-1.6) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178449_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9134
Sequence Type Exemplar sequence
Representative Public ID C53B4.8 NCBI
Target Description C53B4.8 /REP_DB=WormBase Gene ID /WP=CE20601 /TR=Q9XTI8 /GB=CAA92458.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069164(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069164 NCBI Caenorhabditis elegans C53B4.8 (C53B4.8) mRNA, complete cds. 11/11 None
GENEFINDER00000020673 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8995495:9000679:1 10/11 None
C53B4.8 ENSEMBL cdna:known chromosome:CEL140:IV:8993104:9000674:1 gene:C53B4.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020660 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8993135-9000680(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C53B4.8
Entrez Gene 177718 Entrez gene
SwissProt Q9XTI8 EMBL-EBI
WormBase CE20601 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94231 Hypothetical protein C53B4.8 [Caenorhabditis elegans] emb|CAA92458.1| Hypothetical protein C53B4.8 [Caenorhabditis elegans] ref|NP_501565.1| C53B4.8 [Caenorhabditis elegans] 0.0
blast CAE74083 Hypothetical protein CBG21738 [Caenorhabditis briggsae] 0.0
blast CAE65910 Hypothetical protein CBG11077 [Caenorhabditis briggsae] 5.0E-60
blast CAE65910 Hypothetical protein CBG11077 [Caenorhabditis briggsae] 1.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:178449_AT
atacttcggtatctgatcttccatttgatattcacgaaattgatgaattgactgaaaaac
aaaaagaagaatccattcgattaacaattcgagaattggcaaaaggaaatggagcacaag
gacatggggcaactggaaaaagagcaaaacgacaagtaatttcattatttggaggaacat
atcaaattgtttttcttaatccgactgtattctctccacaagcatttgcaccaacgatta
acaaattatcagttcttggtcctcttgtgatctcacctcaattattttgtccaagtgtgc
tctcaccacttctcatgtctcttccggttatctcgccacaggttggtaacccattgatct
tctcaccatatgttcttggtccaaatgttctatcagctgctgtattcaatgcatacgttt
tctctccatatgtattatcaccaaatgttatcaatccgtatgtaatgtctccattaattc
tttcaccatttgtattatgtccagatgttctatctccaactgttttatcaggtgttgttt
tatctccttctgtactctcaccgtctattttcacagatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACTTCGGTATCTGATCTTCCATT 691 23 1571 Antisense
GTATTCTCTCCACAAGCATTTGCAC 156 453 1777 Antisense
ATTATCAGTTCTTGGTCCTCTTGTG 51 15 1815 Antisense
TCCTCTTGTGATCTCACCTCAATTA 138 593 1830 Antisense
TATTTTGTCCAAGTGTGCTCTCACC 302 663 1853 Antisense
GGTTATCTCGCCACAGGTTGGTAAC 294 505 1896 Antisense
GTTGGTAACCCATTGATCTTCTCAC 172 437 1912 Antisense
GGTCCAAATGTTCTATCAGCTGCTG 624 501 1948 Antisense
AATGTCTCCATTAATTCTTTCACCA 45 165 2034 Antisense
CAGGTGTTGTTTTATCTCCTTCTGT 32 209 2099 Antisense
CTCTCACCGTCTATTTTCACAGATT 321 231 2125 Antisense
Sequence Source Affymetrix Proprietary Database