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[178436_at]

Description F28G4.5 /REP_DB=WormBase Gene ID /WP=CE15923 /TR=O17855 /GB=CAB07608.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 26      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 5.1
(-3)
A 		10.9
(-0.9)
A 		21.9
(3)
A 		18.4
(1.8)
A 		17
B 34
(0.6)
A 		51.2
(3)
A 		8.8
(-3)
A 		32.6
(0.4)
A 		42
C 5
(-3)
A 		8
(-2.5)
A 		24
(0.2)
A 		40.8
(3)
A 		36
avr 14.7
(-3) 		23.4
(0.3) 		18.2
(-1.7) 		30.6
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 1.7
(-2.8)
A 		1.1
(-3)
A 		9.7
(0.1)
A 		9.7
(0.1)
A 		4.3
(-1.9)
A 		17.9
(3)
A 		17
B 4.6
(-2.5)
A 		3.1
(-3)
A 		12.8
(0.2)
A 		4.8
(-2.4)
A 		17.9
(1.9)
A 		21.4
(3)
A 		18
C 3.9
(-2.6)
A 		19.8
(3)
A 		2.8
(-3)
A 		6.5
(-1.7)
A 		10.5
(-0.3)
A 		18.3
(2.5)
A 		17
avr 3.4
(-3) 		8
(-1.3) 		8.4
(-1.1) 		7
(-1.6) 		10.9
(-0.2) 		19.2
(3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.8
(-3)
A 		2.4
(-2.1)
A 		5.7
(3)
A 		2.7
(-1.6)
A 		4
B 10.8
(2)
A 		12.6
(3)
A 		2
(-3)
A 		6.9
(-0.2)
A 		11
C 1.6
(-3)
A 		2.3
(-2.4)
A 		8.4
(3)
A 		6.9
(1.7)
A 		7
avr 4.7
(-3) 		5.8
(3) 		5.4
(0.8) 		5.5
(1.4) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.3
(-3)
A 		0.4
(-2.9)
A 		2.1
(-0.4)
A 		2.5
(0.2)
A 		1.3
(-1.5)
A 		4.4
(3)
A 		4
B 1.6
(-2.5)
A 		1
(-3)
A 		3.1
(-1.2)
A 		1.3
(-2.7)
A 		7.3
(2.4)
A 		8
(3)
A 		7
C 1.4
(-2.2)
A 		6
(2.9)
A 		0.7
(-3)
A 		1.3
(-2.3)
A 		3.4
(0)
A 		6.1
(3)
A 		5
avr 1.1
(-3) 		2.5
(-1.4) 		2
(-1.9) 		1.7
(-2.3) 		4
(0.4) 		6.2
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178436_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13004
Sequence Type Exemplar sequence
Representative Public ID F28G4.5 NCBI
Target Description F28G4.5 /REP_DB=WormBase Gene ID /WP=CE15923 /TR=O17855 /GB=CAB07608.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074712(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074712 NCBI Caenorhabditis elegans F28G4.5 (F28G4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000032998 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16291371:16294737:1 11/11 None
F28G4.5 ENSEMBL cdna:known chromosome:CEL140:V:16291371:16294737:1 gene:F28G4.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032996 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16291370-16294737(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28G4.5
Entrez Gene 185083 Entrez gene
SwissProt O17855 EMBL-EBI
WormBase CE15923 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1640542_AT dm
DROSGENOME1:150306_AT dm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16747 transferase activity, transferring groups other than amino-acyl groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07608 Hypothetical protein F28G4.5 [Caenorhabditis elegans] ref|NP_507113.1| F28G4.5 [Caenorhabditis elegans] 0.0
blast CAB07609 Hypothetical protein C31A11.1 [Caenorhabditis elegans] emb|CAB05693.1| Hypothetical protein C31A11.1 [Caenorhabditis elegans] ref|NP_507114.1| C31A11.1 [Caenorhabditis elegans] 0.0
blast CAB05687 Hypothetical protein C31A11.5 [Caenorhabditis elegans] ref|NP_507118.1| C31A11.5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 3.4E-6
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 8.9E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507113.1 13 4-26,183-205,225-244,257-279,312-334,357-379,420-442,449-471,494-511,523-545,565-587,600-622,635-657
F28G4.5 13 4-26,183-205,225-244,257-279,312-334,357-379,420-442,449-471,494-511,523-545,565-587,600-622,635-657
GENEFINDER00000032998 13 4-26,170-192,212-231,244-263,283-305,329-351,392-414,421-443,466-483,495-517,537-559,572-594,607-629
Sequence
Target Sequence 
>C. ELEGANS:178436_AT
ttcgctggcagctttatgcctatttttaacatatgattacgataagagaatcacgtggag
taacttctcccgtgcaacctattacaacttttcgcgtattgcctgggcaattgcagtttc
ttgggtcattgttgctaaccatatgggatggggaggaccaattgatgcattcatgtccca
tccaatttggcaaccatttggaaggctttcgtattgcgcctacattgttcattatgtggt
tttgtacttttacctaatgataggggatggcccacttcatttttattctacttttcagct
tttcatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGCTGGCAGCTTTATGCCTATTT 593 695 1614 Antisense
CACGTGGAGTAACTTCTCCCGTGCA 519 197 1665 Antisense
CGTGCAACCTATTACAACTTTTCGC 89 243 1684 Antisense
TTACAACTTTTCGCGTATTGCCTGG 586 173 1695 Antisense
ATTGCCTGGGCAATTGCAGTTTCTT 127 7 1711 Antisense
ATTGCAGTTTCTTGGGTCATTGTTG 259 5 1723 Antisense
AATTGATGCATTCATGTCCCATCCA 120 179 1773 Antisense
GGAAGGCTTTCGTATTGCGCCTACA 186 533 1813 Antisense
GTTCATTATGTGGTTTTGTACTTTT 170 445 1840 Antisense
GATAGGGGATGGCCCACTTCATTTT 306 425 1872 Antisense
TATTCTACTTTTCAGCTTTTCATGT 160 665 1897 Antisense
Sequence Source Affymetrix Proprietary Database